Electrophoresis in the presence of Coomassie brilliant blue R-250 stains polyacrylamide gels during protein fractionation

Julian Borejdo, Carroll Flynn

Research output: Contribution to journalArticlepeer-review

12 Scopus citations

Abstract

A method of staining polyacrylamide gels in which the dye is electrophoresed together with the sample is proposed. The method cuts short and simplifies the conventional electrophoresis procedure by eliminating the separate poststaining step. In the gels run in the presence of sodium dodecyl sulfate, the method produces protein staining patterns which are quantitatively identical to the ones obtained by conventional staining procedure. Additional advantages of the method are easy control over the degree of staining and homogenous staining independent of the gel thickness and concentration of the dye.

Original languageEnglish
Pages (from-to)84-86
Number of pages3
JournalAnalytical Biochemistry
Volume140
Issue number1
DOIs
StatePublished - Jul 1984

Keywords

  • Coomassie blue stain
  • gel staining
  • polyacrylamide gel electrophoresis

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