EFG1 null mutants of Candida albicans switch but cannot express the complete phenotype of white-phase budding cells

Srikantha Thyagarajan, Luong K. Tsai, Karla Daniels, David R. Soll

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85 Citations (Scopus)

Abstract

The Candida albicans gene EFG1 encodes a putative trans-acting factor. In strain WO-1, which undergoes the white-opaque transition, EFG1 is transcribed as a 3.2-kb mRNA in white-phase cells and a less-abundant 2.2-kb mRNA in opaque-phase cells, cDNA sequencing and 5' rapid amplification of cDNA ends analysis demonstrate that the major difference in molecular mass of the two transcripts is due to different transcription start sites. EFG1 null mutants form opaque-phase colonies and express the opaque-phase cell phenotype at 25°C. When shifted from 25 to 42°C, mutant opaque-phase cells undergo phenotypic commitment to the white phase, which includes deactivation of the opaque-phase-specific gene OP4 and activation of the white-phase- specific gene WH11, as do wild-type opaque-phase cells. After the commitment event, EFG1 null mutant cells form daughter cells which have the smooth (pimpleless) surface of white-phase cells but the elongate morphology of opaque-phase cells. Taken together, these results demonstrate that EFG1 expression is not essential for the switch event per se, but is essential for a subset of phenotypic characteristics necessary for the full expression of the phenotype of white-phase cells. These results demonstrate that EFG1 is not the site of the switch event, but is, rather, downstream of the switch event.

Original languageEnglish
Pages (from-to)1580-1591
Number of pages12
JournalJournal of Bacteriology
Volume182
Issue number6
DOIs
StatePublished - 1 Mar 2000

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Candida albicans
Phenotype
Complementary DNA
Null Lymphocytes
Messenger RNA
Trans-Activators
Transcription Initiation Site
Transcriptional Activation
Genes

Cite this

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title = "EFG1 null mutants of Candida albicans switch but cannot express the complete phenotype of white-phase budding cells",
abstract = "The Candida albicans gene EFG1 encodes a putative trans-acting factor. In strain WO-1, which undergoes the white-opaque transition, EFG1 is transcribed as a 3.2-kb mRNA in white-phase cells and a less-abundant 2.2-kb mRNA in opaque-phase cells, cDNA sequencing and 5' rapid amplification of cDNA ends analysis demonstrate that the major difference in molecular mass of the two transcripts is due to different transcription start sites. EFG1 null mutants form opaque-phase colonies and express the opaque-phase cell phenotype at 25°C. When shifted from 25 to 42°C, mutant opaque-phase cells undergo phenotypic commitment to the white phase, which includes deactivation of the opaque-phase-specific gene OP4 and activation of the white-phase- specific gene WH11, as do wild-type opaque-phase cells. After the commitment event, EFG1 null mutant cells form daughter cells which have the smooth (pimpleless) surface of white-phase cells but the elongate morphology of opaque-phase cells. Taken together, these results demonstrate that EFG1 expression is not essential for the switch event per se, but is essential for a subset of phenotypic characteristics necessary for the full expression of the phenotype of white-phase cells. These results demonstrate that EFG1 is not the site of the switch event, but is, rather, downstream of the switch event.",
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EFG1 null mutants of Candida albicans switch but cannot express the complete phenotype of white-phase budding cells. / Thyagarajan, Srikantha; Tsai, Luong K.; Daniels, Karla; Soll, David R.

In: Journal of Bacteriology, Vol. 182, No. 6, 01.03.2000, p. 1580-1591.

Research output: Contribution to journalArticleResearchpeer-review

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