Efficient processing and export of human growth hormone by heat labile enterotoxin chain B signal sequence

Anuja Ghorpade, Lalit C. Garg

Research output: Contribution to journalArticle

16 Scopus citations

Abstract

The heat-labile enterotoxin chain B (LTB) signal sequence was used for the processing and export of human growth hormone (hGH). The protein was completely processed and exported across the cell membrane to accumulate in the periplasmic space in Escherichia coli. The human growth hormone cDNA was cloned as a PCR amplified fragment under the control of tac promoter and translationally fused to the LTB signal sequence. The rate of processing of hGH under the control of the LTB signal sequence was equal to or more than the rate of induction of expression, indicating efficient processing. The receptor binding activity of the processed periplasmic protein was established in a radio receptor assay.

Original languageEnglish
Pages (from-to)61-65
Number of pages5
JournalFEBS Letters
Volume330
Issue number1
DOIs
StatePublished - 6 Sep 1993

Keywords

  • Export
  • Human growth hormone
  • Processing
  • Signal sequence

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