Efficient expression, processing and secretion of a biologically active mammalian protein by Vibrio cholerae

Anuja Ghorpade; Lalit C. Garg

doi:10.1016/0014-5793(96)00475-9

Efficient expression, processing and secretion of a biologically active mammalian protein by Vibrio cholerae

Anuja Ghorpade, Lalit C. Garg

Graduate School of Biomedical Sciences

Research output: Contribution to journal › Article › peer-review

6 Scopus citations

Abstract

The use of Vibrio cholerae as a secretory expression system for the expression of a mammalian protein, namely human growth hormone, under the control of the heat labile enterotoxin chain B signal sequence is reported. The protein is efficiently expressed and processed. The mature protein is exported to the periplasm after which it is secreted to the extracellular milieu. The expressed and secreted hGH actively binds to its receptor as established by its receptor binding activity. The biological activity of the protein is demonstrated in vitro in a Nb2 proliferation assay.

Original language	English
Pages (from-to)	167-170
Number of pages	4
Journal	FEBS Letters
Volume	387
Issue number	2-3
DOIs	https://doi.org/10.1016/0014-5793(96)00475-9
State	Published - 3 Jun 1996

Keywords

Human growth hormone
Nb2 cell bioassay
Secretory expression
Signal sequence

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10.1016/0014-5793(96)00475-9

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title = "Efficient expression, processing and secretion of a biologically active mammalian protein by Vibrio cholerae",

abstract = "The use of Vibrio cholerae as a secretory expression system for the expression of a mammalian protein, namely human growth hormone, under the control of the heat labile enterotoxin chain B signal sequence is reported. The protein is efficiently expressed and processed. The mature protein is exported to the periplasm after which it is secreted to the extracellular milieu. The expressed and secreted hGH actively binds to its receptor as established by its receptor binding activity. The biological activity of the protein is demonstrated in vitro in a Nb2 proliferation assay.",

keywords = "Human growth hormone, Nb2 cell bioassay, Secretory expression, Signal sequence",

author = "Anuja Ghorpade and Garg, {Lalit C.}",

note = "Funding Information: Acknowledgements: This work was supported by a grant from the Department of Biotechnology, Government of India. We acknowledge Dr. Rahul Pal for help in receptor assays, and Dr. Ayub Quadri and Mr. K.K. Sarin for their help in the Nb2 bioassay. The authors are grateful to Dr. S.K. Basu for his critical comments on the manuscript and constant encouragement. Technical assistance by Rambodh and Ved Prakash is acknowledged.",

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doi = "10.1016/0014-5793(96)00475-9",

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T1 - Efficient expression, processing and secretion of a biologically active mammalian protein by Vibrio cholerae

AU - Ghorpade, Anuja

AU - Garg, Lalit C.

N1 - Funding Information: Acknowledgements: This work was supported by a grant from the Department of Biotechnology, Government of India. We acknowledge Dr. Rahul Pal for help in receptor assays, and Dr. Ayub Quadri and Mr. K.K. Sarin for their help in the Nb2 bioassay. The authors are grateful to Dr. S.K. Basu for his critical comments on the manuscript and constant encouragement. Technical assistance by Rambodh and Ved Prakash is acknowledged.

PY - 1996/6/3

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N2 - The use of Vibrio cholerae as a secretory expression system for the expression of a mammalian protein, namely human growth hormone, under the control of the heat labile enterotoxin chain B signal sequence is reported. The protein is efficiently expressed and processed. The mature protein is exported to the periplasm after which it is secreted to the extracellular milieu. The expressed and secreted hGH actively binds to its receptor as established by its receptor binding activity. The biological activity of the protein is demonstrated in vitro in a Nb2 proliferation assay.

AB - The use of Vibrio cholerae as a secretory expression system for the expression of a mammalian protein, namely human growth hormone, under the control of the heat labile enterotoxin chain B signal sequence is reported. The protein is efficiently expressed and processed. The mature protein is exported to the periplasm after which it is secreted to the extracellular milieu. The expressed and secreted hGH actively binds to its receptor as established by its receptor binding activity. The biological activity of the protein is demonstrated in vitro in a Nb2 proliferation assay.

KW - Human growth hormone

KW - Nb2 cell bioassay

KW - Secretory expression

KW - Signal sequence

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Efficient expression, processing and secretion of a biologically active mammalian protein by Vibrio cholerae

Abstract

Keywords

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