Abstract
The use of Vibrio cholerae as a secretory expression system for the expression of a mammalian protein, namely human growth hormone, under the control of the heat labile enterotoxin chain B signal sequence is reported. The protein is efficiently expressed and processed. The mature protein is exported to the periplasm after which it is secreted to the extracellular milieu. The expressed and secreted hGH actively binds to its receptor as established by its receptor binding activity. The biological activity of the protein is demonstrated in vitro in a Nb2 proliferation assay.
| Original language | English |
|---|---|
| Pages (from-to) | 167-170 |
| Number of pages | 4 |
| Journal | FEBS Letters |
| Volume | 387 |
| Issue number | 2-3 |
| DOIs | |
| State | Published - 3 Jun 1996 |
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Keywords
- Human growth hormone
- Nb2 cell bioassay
- Secretory expression
- Signal sequence
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Efficient expression, processing and secretion of a biologically active mammalian protein by Vibrio cholerae. / Ghorpade, Anuja; Garg, Lalit C.
In: FEBS Letters, Vol. 387, No. 2-3, 03.06.1996, p. 167-170.Research output: Contribution to journal › Article
TY - JOUR
T1 - Efficient expression, processing and secretion of a biologically active mammalian protein by Vibrio cholerae
AU - Ghorpade, Anuja
AU - Garg, Lalit C.
PY - 1996/6/3
Y1 - 1996/6/3
N2 - The use of Vibrio cholerae as a secretory expression system for the expression of a mammalian protein, namely human growth hormone, under the control of the heat labile enterotoxin chain B signal sequence is reported. The protein is efficiently expressed and processed. The mature protein is exported to the periplasm after which it is secreted to the extracellular milieu. The expressed and secreted hGH actively binds to its receptor as established by its receptor binding activity. The biological activity of the protein is demonstrated in vitro in a Nb2 proliferation assay.
AB - The use of Vibrio cholerae as a secretory expression system for the expression of a mammalian protein, namely human growth hormone, under the control of the heat labile enterotoxin chain B signal sequence is reported. The protein is efficiently expressed and processed. The mature protein is exported to the periplasm after which it is secreted to the extracellular milieu. The expressed and secreted hGH actively binds to its receptor as established by its receptor binding activity. The biological activity of the protein is demonstrated in vitro in a Nb2 proliferation assay.
KW - Human growth hormone
KW - Nb2 cell bioassay
KW - Secretory expression
KW - Signal sequence
UR - http://www.scopus.com/inward/record.url?scp=0029888526&partnerID=8YFLogxK
U2 - 10.1016/0014-5793(96)00475-9
DO - 10.1016/0014-5793(96)00475-9
M3 - Article
C2 - 8674542
AN - SCOPUS:0029888526
VL - 387
SP - 167
EP - 170
JO - FEBS Letters
JF - FEBS Letters
SN - 0014-5793
IS - 2-3
ER -