TY - JOUR
T1 - Efficacy of hypochlorous acid scavengers in the prevention of protein carbonyl formation
AU - Yan, Liang Jun
AU - Traber, Maret G.
AU - Kobuchi, Hirotsugu
AU - Matsugo, Seiichi
AU - Tritschler, Hans J.
AU - Packer, Lester
PY - 1996/3/15
Y1 - 1996/3/15
N2 - We observed that protein (bovine serum albumin) carbonyl content increases upon hypochlorite oxidation, and this increase is inhibited in a concentration-dependent manner in the presence of hypochlorite scavengers. Based on this observation, we tested whether some known hypochlorite scavengers (lipoic acid, cysteine, and glutathione) and some other antioxidants (uric acid, ascorbic acid, α-tocopherol, and probucol) could prevent protein carbonyl formation. N-acetylcysteine, dihydrolipoic acid, cysteine, and glutathione (reduced form, GSH), which otherwise could not be tested in a previously reported 5-thio-2-nitro-benzoic acid test system, were successfully evaluated in our assay. The hypochlorite scavenging capacity of different compounds, compared by determining the IC50 (concentration which produces 50% inhibition), showed that the compounds tested have the following potency: dihydrolipoic acid > GSH, N-acetylcysteine > cysteine > S-methyl glutathione > lipoic acid, ascorbic acid > cystine, GSSG, and uric acid. No scavenging ability was observed for either α-tocopherol or probucol.
AB - We observed that protein (bovine serum albumin) carbonyl content increases upon hypochlorite oxidation, and this increase is inhibited in a concentration-dependent manner in the presence of hypochlorite scavengers. Based on this observation, we tested whether some known hypochlorite scavengers (lipoic acid, cysteine, and glutathione) and some other antioxidants (uric acid, ascorbic acid, α-tocopherol, and probucol) could prevent protein carbonyl formation. N-acetylcysteine, dihydrolipoic acid, cysteine, and glutathione (reduced form, GSH), which otherwise could not be tested in a previously reported 5-thio-2-nitro-benzoic acid test system, were successfully evaluated in our assay. The hypochlorite scavenging capacity of different compounds, compared by determining the IC50 (concentration which produces 50% inhibition), showed that the compounds tested have the following potency: dihydrolipoic acid > GSH, N-acetylcysteine > cysteine > S-methyl glutathione > lipoic acid, ascorbic acid > cystine, GSSG, and uric acid. No scavenging ability was observed for either α-tocopherol or probucol.
KW - bovine serum albumin (BSA)
KW - hypochlorous acid
KW - protein carbonyls
KW - scavengers
UR - http://www.scopus.com/inward/record.url?scp=0029866363&partnerID=8YFLogxK
U2 - 10.1006/abbi.1996.0130
DO - 10.1006/abbi.1996.0130
M3 - Article
C2 - 8619623
AN - SCOPUS:0029866363
SN - 0003-9861
VL - 327
SP - 330
EP - 334
JO - Archives of Biochemistry and Biophysics
JF - Archives of Biochemistry and Biophysics
IS - 2
ER -