Effects of smokeless tobacco on chemically transformed hamster oral keratinocytes: Role of angiotensin i-converting enzyme

Günther Müns; Jamboor K. Vishwanatha; Israel Rubinstein

doi:10.1093/carcin/15.7.1325

Effects of smokeless tobacco on chemically transformed hamster oral keratinocytes: Role of angiotensin i-converting enzyme

Günther Müns, Jamboor K. Vishwanatha, Israel Rubinstein

Research output: Contribution to journal › Article › peer-review

22 Scopus citations

Abstract

The purpose of this study was to determine whether exposure of chemically transformed golden Syrian hamster oral epidermoid carcinoma cell (HCPC-1) cultures to smokeless tobacco extract (STE) is associated with a decrease in specific angiotensin I-converting enzyme (ACE) activity and whether this decrease potentiates bradykinin-induced cell growth. We found that STE induced a significant concentration- and time-dependent decrease in ACE activity in cultured HCPC-1 cells (P <0.05). STE alone had no significant effect on cell number. Bradykinin alone induced a slight, but significant, increase in cell number (P <0.05). These effects were signific potentiated by STE (P <0.01). We conclude that STE potentiates bradykinin-induced HCPC-1 cell growth, in part by attenuating specific ACE activity in these cells.

Original language	English
Pages (from-to)	1325-1327
Number of pages	3
Journal	Carcinogenesis
Volume	15
Issue number	7
DOIs	https://doi.org/10.1093/carcin/15.7.1325
State	Published - Jul 1994

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10.1093/carcin/15.7.1325

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@article{06a677197dfc4910af8057f9bbc8e571,

title = "Effects of smokeless tobacco on chemically transformed hamster oral keratinocytes: Role of angiotensin i-converting enzyme",

abstract = "The purpose of this study was to determine whether exposure of chemically transformed golden Syrian hamster oral epidermoid carcinoma cell (HCPC-1) cultures to smokeless tobacco extract (STE) is associated with a decrease in specific angiotensin I-converting enzyme (ACE) activity and whether this decrease potentiates bradykinin-induced cell growth. We found that STE induced a significant concentration- and time-dependent decrease in ACE activity in cultured HCPC-1 cells (P <0.05). STE alone had no significant effect on cell number. Bradykinin alone induced a slight, but significant, increase in cell number (P <0.05). These effects were signific potentiated by STE (P <0.01). We conclude that STE potentiates bradykinin-induced HCPC-1 cell growth, in part by attenuating specific ACE activity in these cells.",

author = "G{\"u}nther M{\"u}ns and Vishwanatha, {Jamboor K.} and Israel Rubinstein",

note = "Funding Information: We thank Mary niig for technical assistance and Dr Wong for providing the HCPC-1 cells. This study was supported, in part, by National Institutes of Health grants DE-10347 and GM-46459, a Grant-in-Aid from the American Heart Association-Nebraska Affiliate (NE-92-GS-6) and a Post-Doctoral Fellowship from the American Heart Association-Nebraska Affiliate and the Milhdm Foundation.",

year = "1994",

month = jul,

doi = "10.1093/carcin/15.7.1325",

language = "English",

volume = "15",

pages = "1325--1327",

journal = "Carcinogenesis",

issn = "0143-3334",

publisher = "Oxford University Press",

number = "7",

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T1 - Effects of smokeless tobacco on chemically transformed hamster oral keratinocytes

T2 - Role of angiotensin i-converting enzyme

AU - Müns, Günther

AU - Vishwanatha, Jamboor K.

AU - Rubinstein, Israel

N1 - Funding Information: We thank Mary niig for technical assistance and Dr Wong for providing the HCPC-1 cells. This study was supported, in part, by National Institutes of Health grants DE-10347 and GM-46459, a Grant-in-Aid from the American Heart Association-Nebraska Affiliate (NE-92-GS-6) and a Post-Doctoral Fellowship from the American Heart Association-Nebraska Affiliate and the Milhdm Foundation.

PY - 1994/7

Y1 - 1994/7

N2 - The purpose of this study was to determine whether exposure of chemically transformed golden Syrian hamster oral epidermoid carcinoma cell (HCPC-1) cultures to smokeless tobacco extract (STE) is associated with a decrease in specific angiotensin I-converting enzyme (ACE) activity and whether this decrease potentiates bradykinin-induced cell growth. We found that STE induced a significant concentration- and time-dependent decrease in ACE activity in cultured HCPC-1 cells (P <0.05). STE alone had no significant effect on cell number. Bradykinin alone induced a slight, but significant, increase in cell number (P <0.05). These effects were signific potentiated by STE (P <0.01). We conclude that STE potentiates bradykinin-induced HCPC-1 cell growth, in part by attenuating specific ACE activity in these cells.

AB - The purpose of this study was to determine whether exposure of chemically transformed golden Syrian hamster oral epidermoid carcinoma cell (HCPC-1) cultures to smokeless tobacco extract (STE) is associated with a decrease in specific angiotensin I-converting enzyme (ACE) activity and whether this decrease potentiates bradykinin-induced cell growth. We found that STE induced a significant concentration- and time-dependent decrease in ACE activity in cultured HCPC-1 cells (P <0.05). STE alone had no significant effect on cell number. Bradykinin alone induced a slight, but significant, increase in cell number (P <0.05). These effects were signific potentiated by STE (P <0.01). We conclude that STE potentiates bradykinin-induced HCPC-1 cell growth, in part by attenuating specific ACE activity in these cells.

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U2 - 10.1093/carcin/15.7.1325

DO - 10.1093/carcin/15.7.1325

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AN - SCOPUS:0028245343

SN - 0143-3334

VL - 15

SP - 1325

EP - 1327

JO - Carcinogenesis

JF - Carcinogenesis

IS - 7

ER -

Effects of smokeless tobacco on chemically transformed hamster oral keratinocytes: Role of angiotensin i-converting enzyme

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