Effects of Shu Gan Jian Pi formula on rats with carbon tetrachloride-induced Liver fibrosis using serum metabonomics based on gas chromatography-time of flight mass spectrometry

Hui Jiang, Xiu Juan Qin, Wei Ping Li, Rong Ma, Ting Wang, Zhu Qing Li

Research output: Contribution to journalArticleResearchpeer-review

4 Citations (Scopus)

Abstract

Liver fibrosis is a common stage in the majority of chronic liver diseases, regardless of the etiology, and its progression may lead to hepatic cirrhosis or hepatocellular carcinoma. Metabolomics, a powerful approach in systems biology, is a discipline used to qualitatively and quantitatively analyze the small molecule metabolites of cells at specific times and under certain conditions. The present study aimed to investigate serum metabolic changes following Shu Gan Jian Pi formula (SGJPF) treatment of carbon tetrachloride (CCl4)-induced liver fibrosis in rats using gas chromatography-time of flight mass spectrometry (GC-TOFMS). In addition, the potential mechanisms were explored. Rat liver fibrosis was induced by twice-weekly subcutaneous CCl4 injection for 12 continuous weeks. During the same period, the SGJPF group received 16.2 g/kg body weight SGJPF, diluted in water, once a day for 12 weeks. Rats in the control and model groups received oral administration of the same volume of saline solution. Serum samples from the control, model and SGJPF groups were collected after 12 weeks of treatment, and metabolic profile alterations were analyzed by GC-TOF/MS. Metabolic profile analysis indicated that clustering differed between the three groups and the following 12 metabolites were detected in the serum of all three groups: Isoleucine; L-malic acid; D-erythro-sphingosine; putrescine; malonic acid; 3,6-anhydro-D-galactose, α-ketoglutaric acid; ornithine; glucose; hippuric acid; tetrahydrocorticosterone; and fucose. The results demonstrated that SGJPF treatment mitigated the effects of CCl4-induced liver fibrosis on biomarker levels, thus indicating that SGJPF may have a therapeutic effect on CCl4-induced liver fibrosis in rats. The mechanism may involve the regulation of energy, amino acid, sphingolipid, cytochrome P450, glucose and water-electrolyte metabolism.

Original languageEnglish
Pages (from-to)3901-3909
Number of pages9
JournalMolecular Medicine Reports
Volume16
Issue number4
DOIs
StatePublished - 1 Oct 2017

Fingerprint

Metabolomics
Carbon Tetrachloride
Liver Cirrhosis
Gas chromatography
Gas Chromatography
Liver
Mass spectrometry
Rats
Mass Spectrometry
Serum
Metabolome
Metabolites
Ketoglutaric Acids
Glucose
Sphingolipids
Ornithine
Putrescine
Systems Biology
Fucose
Water

Keywords

  • Gas chromatography-time of flight mass spectrometry
  • Liver fibrosis
  • Metabonomics
  • Serum
  • Shu Gan Jian Pi formula

Cite this

@article{102933a9c9b54852bbb4b1ae7f6d442e,
title = "Effects of Shu Gan Jian Pi formula on rats with carbon tetrachloride-induced Liver fibrosis using serum metabonomics based on gas chromatography-time of flight mass spectrometry",
abstract = "Liver fibrosis is a common stage in the majority of chronic liver diseases, regardless of the etiology, and its progression may lead to hepatic cirrhosis or hepatocellular carcinoma. Metabolomics, a powerful approach in systems biology, is a discipline used to qualitatively and quantitatively analyze the small molecule metabolites of cells at specific times and under certain conditions. The present study aimed to investigate serum metabolic changes following Shu Gan Jian Pi formula (SGJPF) treatment of carbon tetrachloride (CCl4)-induced liver fibrosis in rats using gas chromatography-time of flight mass spectrometry (GC-TOFMS). In addition, the potential mechanisms were explored. Rat liver fibrosis was induced by twice-weekly subcutaneous CCl4 injection for 12 continuous weeks. During the same period, the SGJPF group received 16.2 g/kg body weight SGJPF, diluted in water, once a day for 12 weeks. Rats in the control and model groups received oral administration of the same volume of saline solution. Serum samples from the control, model and SGJPF groups were collected after 12 weeks of treatment, and metabolic profile alterations were analyzed by GC-TOF/MS. Metabolic profile analysis indicated that clustering differed between the three groups and the following 12 metabolites were detected in the serum of all three groups: Isoleucine; L-malic acid; D-erythro-sphingosine; putrescine; malonic acid; 3,6-anhydro-D-galactose, α-ketoglutaric acid; ornithine; glucose; hippuric acid; tetrahydrocorticosterone; and fucose. The results demonstrated that SGJPF treatment mitigated the effects of CCl4-induced liver fibrosis on biomarker levels, thus indicating that SGJPF may have a therapeutic effect on CCl4-induced liver fibrosis in rats. The mechanism may involve the regulation of energy, amino acid, sphingolipid, cytochrome P450, glucose and water-electrolyte metabolism.",
keywords = "Gas chromatography-time of flight mass spectrometry, Liver fibrosis, Metabonomics, Serum, Shu Gan Jian Pi formula",
author = "Hui Jiang and Qin, {Xiu Juan} and Li, {Wei Ping} and Rong Ma and Ting Wang and Li, {Zhu Qing}",
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language = "English",
volume = "16",
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Effects of Shu Gan Jian Pi formula on rats with carbon tetrachloride-induced Liver fibrosis using serum metabonomics based on gas chromatography-time of flight mass spectrometry. / Jiang, Hui; Qin, Xiu Juan; Li, Wei Ping; Ma, Rong; Wang, Ting; Li, Zhu Qing.

In: Molecular Medicine Reports, Vol. 16, No. 4, 01.10.2017, p. 3901-3909.

Research output: Contribution to journalArticleResearchpeer-review

TY - JOUR

T1 - Effects of Shu Gan Jian Pi formula on rats with carbon tetrachloride-induced Liver fibrosis using serum metabonomics based on gas chromatography-time of flight mass spectrometry

AU - Jiang, Hui

AU - Qin, Xiu Juan

AU - Li, Wei Ping

AU - Ma, Rong

AU - Wang, Ting

AU - Li, Zhu Qing

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AB - Liver fibrosis is a common stage in the majority of chronic liver diseases, regardless of the etiology, and its progression may lead to hepatic cirrhosis or hepatocellular carcinoma. Metabolomics, a powerful approach in systems biology, is a discipline used to qualitatively and quantitatively analyze the small molecule metabolites of cells at specific times and under certain conditions. The present study aimed to investigate serum metabolic changes following Shu Gan Jian Pi formula (SGJPF) treatment of carbon tetrachloride (CCl4)-induced liver fibrosis in rats using gas chromatography-time of flight mass spectrometry (GC-TOFMS). In addition, the potential mechanisms were explored. Rat liver fibrosis was induced by twice-weekly subcutaneous CCl4 injection for 12 continuous weeks. During the same period, the SGJPF group received 16.2 g/kg body weight SGJPF, diluted in water, once a day for 12 weeks. Rats in the control and model groups received oral administration of the same volume of saline solution. Serum samples from the control, model and SGJPF groups were collected after 12 weeks of treatment, and metabolic profile alterations were analyzed by GC-TOF/MS. Metabolic profile analysis indicated that clustering differed between the three groups and the following 12 metabolites were detected in the serum of all three groups: Isoleucine; L-malic acid; D-erythro-sphingosine; putrescine; malonic acid; 3,6-anhydro-D-galactose, α-ketoglutaric acid; ornithine; glucose; hippuric acid; tetrahydrocorticosterone; and fucose. The results demonstrated that SGJPF treatment mitigated the effects of CCl4-induced liver fibrosis on biomarker levels, thus indicating that SGJPF may have a therapeutic effect on CCl4-induced liver fibrosis in rats. The mechanism may involve the regulation of energy, amino acid, sphingolipid, cytochrome P450, glucose and water-electrolyte metabolism.

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