TY - JOUR
T1 - Effects of presumptive test reagents on the ability to obtain restriction fragment length polymorphism (RFLP) patterns from human blood and semen stains
AU - Hochmeister, M. N.
AU - Budowle, B.
AU - Baechtel, F. S.
PY - 1991
Y1 - 1991
N2 - Some of the commonly used presumptive test reagents for identification of blood and semen could potentially affect the recovery of intact high- molecular-weight deoxyribonucleic acid (DNA) from evidentiary samples. Thus, the capability of performing restriction fragment length polymorphism (RFLP) analysis on evidentiary samples could be compromised. In order to investigate the potential effects of presumptive test reagents on the DNA present in these samples, bloodstains on cotton and glass were exposed directly to luminol, benzidine, phenolphthalein, o-tolidine, and leucomalachite green, while semen stains and vaginal swabs containing semen were exposed directly to bromochloroindolyl phosphate (BCIP) and sodium thymolphthalein monophosphate (STMP) reagents. The yield gels for DNA quality and quantity and RFLP results indicated that bloodstains exposed to luminol, benzidine dissolved in ethanol, and phenolphthalein, as well as semen stains and vaginal swabs exposed to BCIP and STMP yield RFLP patterns consistent with that of the uncontaminated control. Except for the phenolphthalein treatment, the quantity of extractable, high-molecular-weight DNA obtained was comparable with that of untreated stains. Therefore, evidentiary material purposely or inadvertently contaminated with these reagents can be successfully typed. However, stains exposed to benzidine dissolved in glacial acetic acid, leucomalachite green, and o-tolidine failed to yield high- molecular-weight DNA or to produce any RFLP patterns.
AB - Some of the commonly used presumptive test reagents for identification of blood and semen could potentially affect the recovery of intact high- molecular-weight deoxyribonucleic acid (DNA) from evidentiary samples. Thus, the capability of performing restriction fragment length polymorphism (RFLP) analysis on evidentiary samples could be compromised. In order to investigate the potential effects of presumptive test reagents on the DNA present in these samples, bloodstains on cotton and glass were exposed directly to luminol, benzidine, phenolphthalein, o-tolidine, and leucomalachite green, while semen stains and vaginal swabs containing semen were exposed directly to bromochloroindolyl phosphate (BCIP) and sodium thymolphthalein monophosphate (STMP) reagents. The yield gels for DNA quality and quantity and RFLP results indicated that bloodstains exposed to luminol, benzidine dissolved in ethanol, and phenolphthalein, as well as semen stains and vaginal swabs exposed to BCIP and STMP yield RFLP patterns consistent with that of the uncontaminated control. Except for the phenolphthalein treatment, the quantity of extractable, high-molecular-weight DNA obtained was comparable with that of untreated stains. Therefore, evidentiary material purposely or inadvertently contaminated with these reagents can be successfully typed. However, stains exposed to benzidine dissolved in glacial acetic acid, leucomalachite green, and o-tolidine failed to yield high- molecular-weight DNA or to produce any RFLP patterns.
KW - RFLP
KW - body fluids
KW - criminalistics
KW - deoxyribonucleic acid (DNA)
KW - identification of blood
KW - identification of semen
KW - presumptive tests
UR - http://www.scopus.com/inward/record.url?scp=0026329673&partnerID=8YFLogxK
U2 - 10.1520/jfs13074j
DO - 10.1520/jfs13074j
M3 - Article
C2 - 1677394
AN - SCOPUS:0026329673
VL - 36
SP - 656
EP - 661
JO - Journal of forensic sciences
JF - Journal of forensic sciences
SN - 0022-1198
IS - 3
ER -