Effects of metallic silver particles on resonance energy transfer in labeled bovine serum albumin

Joanna Malicka; Ignacy Gryczynski; Jozef Kusba; Yibing Shen; Joseph R. Lakowicz

doi:10.1016/S0006-291X(02)00555-7

Effects of metallic silver particles on resonance energy transfer in labeled bovine serum albumin

Joanna Malicka, Ignacy Gryczynski, Jozef Kusba, Yibing Shen, Joseph R. Lakowicz

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39 Scopus citations

Abstract

Resonance energy transfer (RET) is widely used to detect proximity between biomolecules. In transparent solution the maximum donor-to-acceptor distance for RET is about 70 Å. We measured the effects of metallic silver island films on RET from the intrinsic tryptophan of a protein to a bound probe as the acceptor. These preliminary experiments revealed a dramatic increase in the apparent Förster distance increasing from 28.6 to 63 Å. These results suggest the use of silver island films for detecting long range proximity between biomolecules and for biotechnology applications based on RET.

Original language	English
Pages (from-to)	886-892
Number of pages	7
Journal	Biochemical and Biophysical Research Communications
Volume	294
Issue number	4
DOIs	https://doi.org/10.1016/S0006-291X(02)00555-7
State	Published - 2002

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title = "Effects of metallic silver particles on resonance energy transfer in labeled bovine serum albumin",

abstract = "Resonance energy transfer (RET) is widely used to detect proximity between biomolecules. In transparent solution the maximum donor-to-acceptor distance for RET is about 70 {\AA}. We measured the effects of metallic silver island films on RET from the intrinsic tryptophan of a protein to a bound probe as the acceptor. These preliminary experiments revealed a dramatic increase in the apparent F{\"o}rster distance increasing from 28.6 to 63 {\AA}. These results suggest the use of silver island films for detecting long range proximity between biomolecules and for biotechnology applications based on RET.",

author = "Joanna Malicka and Ignacy Gryczynski and Jozef Kusba and Yibing Shen and Lakowicz, {Joseph R.}",

note = "Funding Information: This work was supported by the NIH, National Center for Research Resource, RR-08119.",

year = "2002",

doi = "10.1016/S0006-291X(02)00555-7",

language = "English",

volume = "294",

pages = "886--892",

journal = "Biochemical and Biophysical Research Communications",

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publisher = "Academic Press Inc.",

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T1 - Effects of metallic silver particles on resonance energy transfer in labeled bovine serum albumin

AU - Malicka, Joanna

AU - Gryczynski, Ignacy

AU - Kusba, Jozef

AU - Shen, Yibing

AU - Lakowicz, Joseph R.

N1 - Funding Information: This work was supported by the NIH, National Center for Research Resource, RR-08119.

PY - 2002

Y1 - 2002

N2 - Resonance energy transfer (RET) is widely used to detect proximity between biomolecules. In transparent solution the maximum donor-to-acceptor distance for RET is about 70 Å. We measured the effects of metallic silver island films on RET from the intrinsic tryptophan of a protein to a bound probe as the acceptor. These preliminary experiments revealed a dramatic increase in the apparent Förster distance increasing from 28.6 to 63 Å. These results suggest the use of silver island films for detecting long range proximity between biomolecules and for biotechnology applications based on RET.

AB - Resonance energy transfer (RET) is widely used to detect proximity between biomolecules. In transparent solution the maximum donor-to-acceptor distance for RET is about 70 Å. We measured the effects of metallic silver island films on RET from the intrinsic tryptophan of a protein to a bound probe as the acceptor. These preliminary experiments revealed a dramatic increase in the apparent Förster distance increasing from 28.6 to 63 Å. These results suggest the use of silver island films for detecting long range proximity between biomolecules and for biotechnology applications based on RET.

UR - http://www.scopus.com/inward/record.url?scp=0036289743&partnerID=8YFLogxK

U2 - 10.1016/S0006-291X(02)00555-7

DO - 10.1016/S0006-291X(02)00555-7

M3 - Article

C2 - 12061790

AN - SCOPUS:0036289743

SN - 0006-291X

VL - 294

SP - 886

EP - 892

JO - Biochemical and Biophysical Research Communications

JF - Biochemical and Biophysical Research Communications

IS - 4

ER -

Effects of metallic silver particles on resonance energy transfer in labeled bovine serum albumin

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