Effects of Metallic Silver Particles on Resonance Energy Transfer between Fluorophores Bound to DNA

Joseph R. Lakowicz, Józef Kuśba, Yibing Shen, Joanna Malicka, Sabato D'Auria, Zygmunt Gryczynski, Ignacy Gryczynski

Research output: Contribution to journalArticle

51 Scopus citations

Abstract

We examined the effects of metallic silver island films on resonance energy transfer (RET) between a donor and acceptor bound to double helical DNA. The donor was 4′,6-diamidino-2-phenylindole (DAPI) and the acceptor was propidium iodide (PI). Proximity of the labeled DNA to the silver particles resulted in a dramatic increase in RET as seen from the emission spectra and the donor decay times. Proximity to silver particles results in an increase of the Förster distance from 35 Å to an apparent value of 166 Å. These results suggest a new type of DNA hybridization assays based on RET over distances much longer than the free-space Forster distance.

Original languageEnglish
Pages (from-to)69-77
Number of pages9
JournalJournal of Fluorescence
Volume13
Issue number1
DOIs
StatePublished - Jan 2003

Keywords

  • Fluorescence resonance energy transfer
  • Labeled DNA
  • Radiative decay engineering
  • Silver nanoparticles

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