Effects of Metallic Silver Particles on Resonance Energy Transfer between Fluorophores Bound to DNA

Joseph R. Lakowicz, Józef Kuśba, Yibing Shen, Joanna Malicka, Sabato D'Auria, Zygmunt Gryczynski, Ignacy Gryczynski

Research output: Contribution to journalArticle

51 Citations (Scopus)

Abstract

We examined the effects of metallic silver island films on resonance energy transfer (RET) between a donor and acceptor bound to double helical DNA. The donor was 4′,6-diamidino-2-phenylindole (DAPI) and the acceptor was propidium iodide (PI). Proximity of the labeled DNA to the silver particles resulted in a dramatic increase in RET as seen from the emission spectra and the donor decay times. Proximity to silver particles results in an increase of the Förster distance from 35 Å to an apparent value of 166 Å. These results suggest a new type of DNA hybridization assays based on RET over distances much longer than the free-space Forster distance.

Original languageEnglish
Pages (from-to)69-77
Number of pages9
JournalJournal of Fluorescence
Volume13
Issue number1
DOIs
StatePublished - 1 Jan 2003

Fingerprint

Fluorophores
Energy Transfer
Silver
Energy transfer
energy
DNA
Propidium
Islands
Assays
Values

Keywords

  • Fluorescence resonance energy transfer
  • Labeled DNA
  • Radiative decay engineering
  • Silver nanoparticles

Cite this

Lakowicz, Joseph R. ; Kuśba, Józef ; Shen, Yibing ; Malicka, Joanna ; D'Auria, Sabato ; Gryczynski, Zygmunt ; Gryczynski, Ignacy. / Effects of Metallic Silver Particles on Resonance Energy Transfer between Fluorophores Bound to DNA. In: Journal of Fluorescence. 2003 ; Vol. 13, No. 1. pp. 69-77.
@article{19865ecd06e741d791dad6e7c4937d56,
title = "Effects of Metallic Silver Particles on Resonance Energy Transfer between Fluorophores Bound to DNA",
abstract = "We examined the effects of metallic silver island films on resonance energy transfer (RET) between a donor and acceptor bound to double helical DNA. The donor was 4′,6-diamidino-2-phenylindole (DAPI) and the acceptor was propidium iodide (PI). Proximity of the labeled DNA to the silver particles resulted in a dramatic increase in RET as seen from the emission spectra and the donor decay times. Proximity to silver particles results in an increase of the F{\"o}rster distance from 35 {\AA} to an apparent value of 166 {\AA}. These results suggest a new type of DNA hybridization assays based on RET over distances much longer than the free-space Forster distance.",
keywords = "Fluorescence resonance energy transfer, Labeled DNA, Radiative decay engineering, Silver nanoparticles",
author = "Lakowicz, {Joseph R.} and J{\'o}zef Kuśba and Yibing Shen and Joanna Malicka and Sabato D'Auria and Zygmunt Gryczynski and Ignacy Gryczynski",
year = "2003",
month = "1",
day = "1",
doi = "10.1023/A:1022306630924",
language = "English",
volume = "13",
pages = "69--77",
journal = "Journal of Fluorescence",
issn = "1053-0509",
publisher = "Springer New York",
number = "1",

}

Effects of Metallic Silver Particles on Resonance Energy Transfer between Fluorophores Bound to DNA. / Lakowicz, Joseph R.; Kuśba, Józef; Shen, Yibing; Malicka, Joanna; D'Auria, Sabato; Gryczynski, Zygmunt; Gryczynski, Ignacy.

In: Journal of Fluorescence, Vol. 13, No. 1, 01.01.2003, p. 69-77.

Research output: Contribution to journalArticle

TY - JOUR

T1 - Effects of Metallic Silver Particles on Resonance Energy Transfer between Fluorophores Bound to DNA

AU - Lakowicz, Joseph R.

AU - Kuśba, Józef

AU - Shen, Yibing

AU - Malicka, Joanna

AU - D'Auria, Sabato

AU - Gryczynski, Zygmunt

AU - Gryczynski, Ignacy

PY - 2003/1/1

Y1 - 2003/1/1

N2 - We examined the effects of metallic silver island films on resonance energy transfer (RET) between a donor and acceptor bound to double helical DNA. The donor was 4′,6-diamidino-2-phenylindole (DAPI) and the acceptor was propidium iodide (PI). Proximity of the labeled DNA to the silver particles resulted in a dramatic increase in RET as seen from the emission spectra and the donor decay times. Proximity to silver particles results in an increase of the Förster distance from 35 Å to an apparent value of 166 Å. These results suggest a new type of DNA hybridization assays based on RET over distances much longer than the free-space Forster distance.

AB - We examined the effects of metallic silver island films on resonance energy transfer (RET) between a donor and acceptor bound to double helical DNA. The donor was 4′,6-diamidino-2-phenylindole (DAPI) and the acceptor was propidium iodide (PI). Proximity of the labeled DNA to the silver particles resulted in a dramatic increase in RET as seen from the emission spectra and the donor decay times. Proximity to silver particles results in an increase of the Förster distance from 35 Å to an apparent value of 166 Å. These results suggest a new type of DNA hybridization assays based on RET over distances much longer than the free-space Forster distance.

KW - Fluorescence resonance energy transfer

KW - Labeled DNA

KW - Radiative decay engineering

KW - Silver nanoparticles

UR - http://www.scopus.com/inward/record.url?scp=0346460103&partnerID=8YFLogxK

U2 - 10.1023/A:1022306630924

DO - 10.1023/A:1022306630924

M3 - Article

AN - SCOPUS:0346460103

VL - 13

SP - 69

EP - 77

JO - Journal of Fluorescence

JF - Journal of Fluorescence

SN - 1053-0509

IS - 1

ER -