Effects of Metallic Silver Particles on Resonance Energy Transfer between Fluorophores Bound to DNA

Joseph R. Lakowicz; Józef Kuśba; Yibing Shen; Joanna Malicka; Sabato D'Auria; Zygmunt Gryczynski; Ignacy Gryczynski

doi:10.1023/A:1022306630924

Effects of Metallic Silver Particles on Resonance Energy Transfer between Fluorophores Bound to DNA

Joseph R. Lakowicz, Józef Kuśba, Yibing Shen, Joanna Malicka, Sabato D'Auria, Zygmunt Gryczynski, Ignacy Gryczynski

Research output: Contribution to journal › Article › peer-review

58 Scopus citations

Abstract

We examined the effects of metallic silver island films on resonance energy transfer (RET) between a donor and acceptor bound to double helical DNA. The donor was 4′,6-diamidino-2-phenylindole (DAPI) and the acceptor was propidium iodide (PI). Proximity of the labeled DNA to the silver particles resulted in a dramatic increase in RET as seen from the emission spectra and the donor decay times. Proximity to silver particles results in an increase of the Förster distance from 35 Å to an apparent value of 166 Å. These results suggest a new type of DNA hybridization assays based on RET over distances much longer than the free-space Forster distance.

Original language	English
Pages (from-to)	69-77
Number of pages	9
Journal	Journal of Fluorescence
Volume	13
Issue number	1
DOIs	https://doi.org/10.1023/A:1022306630924
State	Published - Jan 2003

Keywords

Fluorescence resonance energy transfer
Labeled DNA
Radiative decay engineering
Silver nanoparticles

Access to Document

10.1023/A:1022306630924

Cite this

@article{19865ecd06e741d791dad6e7c4937d56,

title = "Effects of Metallic Silver Particles on Resonance Energy Transfer between Fluorophores Bound to DNA",

abstract = "We examined the effects of metallic silver island films on resonance energy transfer (RET) between a donor and acceptor bound to double helical DNA. The donor was 4′,6-diamidino-2-phenylindole (DAPI) and the acceptor was propidium iodide (PI). Proximity of the labeled DNA to the silver particles resulted in a dramatic increase in RET as seen from the emission spectra and the donor decay times. Proximity to silver particles results in an increase of the F{\"o}rster distance from 35 {\AA} to an apparent value of 166 {\AA}. These results suggest a new type of DNA hybridization assays based on RET over distances much longer than the free-space Forster distance.",

keywords = "Fluorescence resonance energy transfer, Labeled DNA, Radiative decay engineering, Silver nanoparticles",

author = "Lakowicz, {Joseph R.} and J{\'o}zef Ku{\'s}ba and Yibing Shen and Joanna Malicka and Sabato D'Auria and Zygmunt Gryczynski and Ignacy Gryczynski",

year = "2003",

month = jan,

doi = "10.1023/A:1022306630924",

language = "English",

volume = "13",

pages = "69--77",

journal = "Journal of Fluorescence",

issn = "1053-0509",

publisher = "Springer New York",

number = "1",

}

TY - JOUR

T1 - Effects of Metallic Silver Particles on Resonance Energy Transfer between Fluorophores Bound to DNA

AU - Lakowicz, Joseph R.

AU - Kuśba, Józef

AU - Shen, Yibing

AU - Malicka, Joanna

AU - D'Auria, Sabato

AU - Gryczynski, Zygmunt

AU - Gryczynski, Ignacy

PY - 2003/1

Y1 - 2003/1

N2 - We examined the effects of metallic silver island films on resonance energy transfer (RET) between a donor and acceptor bound to double helical DNA. The donor was 4′,6-diamidino-2-phenylindole (DAPI) and the acceptor was propidium iodide (PI). Proximity of the labeled DNA to the silver particles resulted in a dramatic increase in RET as seen from the emission spectra and the donor decay times. Proximity to silver particles results in an increase of the Förster distance from 35 Å to an apparent value of 166 Å. These results suggest a new type of DNA hybridization assays based on RET over distances much longer than the free-space Forster distance.

AB - We examined the effects of metallic silver island films on resonance energy transfer (RET) between a donor and acceptor bound to double helical DNA. The donor was 4′,6-diamidino-2-phenylindole (DAPI) and the acceptor was propidium iodide (PI). Proximity of the labeled DNA to the silver particles resulted in a dramatic increase in RET as seen from the emission spectra and the donor decay times. Proximity to silver particles results in an increase of the Förster distance from 35 Å to an apparent value of 166 Å. These results suggest a new type of DNA hybridization assays based on RET over distances much longer than the free-space Forster distance.

KW - Fluorescence resonance energy transfer

KW - Labeled DNA

KW - Radiative decay engineering

KW - Silver nanoparticles

UR - http://www.scopus.com/inward/record.url?scp=0346460103&partnerID=8YFLogxK

U2 - 10.1023/A:1022306630924

DO - 10.1023/A:1022306630924

M3 - Article

AN - SCOPUS:0346460103

SN - 1053-0509

VL - 13

SP - 69

EP - 77

JO - Journal of Fluorescence

JF - Journal of Fluorescence

IS - 1

ER -

Effects of Metallic Silver Particles on Resonance Energy Transfer between Fluorophores Bound to DNA

Abstract

Keywords

Access to Document

Other files and links

Fingerprint

Cite this