Abstract
We examined the effects of metallic silver island films on resonance energy transfer (RET) between a donor and acceptor bound to double helical DNA. The donor was 4′,6-diamidino-2-phenylindole (DAPI) and the acceptor was propidium iodide (PI). Proximity of the labeled DNA to the silver particles resulted in a dramatic increase in RET as seen from the emission spectra and the donor decay times. Proximity to silver particles results in an increase of the Förster distance from 35 Å to an apparent value of 166 Å. These results suggest a new type of DNA hybridization assays based on RET over distances much longer than the free-space Forster distance.
Original language | English |
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Pages (from-to) | 69-77 |
Number of pages | 9 |
Journal | Journal of Fluorescence |
Volume | 13 |
Issue number | 1 |
DOIs | |
State | Published - Jan 2003 |
Keywords
- Fluorescence resonance energy transfer
- Labeled DNA
- Radiative decay engineering
- Silver nanoparticles