Effects of Merocyanine 540-Sensitized Photoirradiation on Proteus mirabilis Growth and Lipopolysaccharide Toxicity

K. Bednarska; P. Kassak; R. Fudala; I. Waczulikova; W. Kaca; C. Watala; L. Sikurova

Effects of Merocyanine 540-Sensitized Photoirradiation on Proteus mirabilis Growth and Lipopolysaccharide Toxicity

K. Bednarska, P. Kassak, R. Fudala, I. Waczulikova, W. Kaca, C. Watala, L. Sikurova

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Abstract

The effect of Nd:YAG laser (30 mW, 532 nm) photoirradiation on the growth and colony formation of two strains of the Gram-negative bacteria Proteus mirabilis, the wild type S1959 and the mutant R45, differing in polysaccharide content in the bacterial cell wall lipopolysaccharide (LPS), was tested in the presence of the photosensitizer merocyanine 540 (MC540). Simultaneous action of MC540 and Nd:YAG laser light showed a differentiated influence depending on LPS structure: their bactericidal effect increased with depletion of the polysaccharide chain in LPS. Moreover, MC540 and laser illumination induced a dramatic increase in lipopolysaccharide toxicity, as monitored by means of the Limulus amebocyte lysate (LAL) test. This effect should be taken into account when applying combined photosensitizer-mediated laser irradiation for the photodynamic sterilization of blood transfusion products.

Original language	English
Pages (from-to)	1212-1216
Number of pages	5
Journal	Laser Physics
Volume	13
Issue number	9
State	Published - Sep 2003

Cite this

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title = "Effects of Merocyanine 540-Sensitized Photoirradiation on Proteus mirabilis Growth and Lipopolysaccharide Toxicity",

abstract = "The effect of Nd:YAG laser (30 mW, 532 nm) photoirradiation on the growth and colony formation of two strains of the Gram-negative bacteria Proteus mirabilis, the wild type S1959 and the mutant R45, differing in polysaccharide content in the bacterial cell wall lipopolysaccharide (LPS), was tested in the presence of the photosensitizer merocyanine 540 (MC540). Simultaneous action of MC540 and Nd:YAG laser light showed a differentiated influence depending on LPS structure: their bactericidal effect increased with depletion of the polysaccharide chain in LPS. Moreover, MC540 and laser illumination induced a dramatic increase in lipopolysaccharide toxicity, as monitored by means of the Limulus amebocyte lysate (LAL) test. This effect should be taken into account when applying combined photosensitizer-mediated laser irradiation for the photodynamic sterilization of blood transfusion products.",

author = "K. Bednarska and P. Kassak and R. Fudala and I. Waczulikova and W. Kaca and C. Watala and L. Sikurova",

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T1 - Effects of Merocyanine 540-Sensitized Photoirradiation on Proteus mirabilis Growth and Lipopolysaccharide Toxicity

AU - Bednarska, K.

AU - Kassak, P.

AU - Fudala, R.

AU - Waczulikova, I.

AU - Kaca, W.

AU - Watala, C.

AU - Sikurova, L.

PY - 2003/9

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N2 - The effect of Nd:YAG laser (30 mW, 532 nm) photoirradiation on the growth and colony formation of two strains of the Gram-negative bacteria Proteus mirabilis, the wild type S1959 and the mutant R45, differing in polysaccharide content in the bacterial cell wall lipopolysaccharide (LPS), was tested in the presence of the photosensitizer merocyanine 540 (MC540). Simultaneous action of MC540 and Nd:YAG laser light showed a differentiated influence depending on LPS structure: their bactericidal effect increased with depletion of the polysaccharide chain in LPS. Moreover, MC540 and laser illumination induced a dramatic increase in lipopolysaccharide toxicity, as monitored by means of the Limulus amebocyte lysate (LAL) test. This effect should be taken into account when applying combined photosensitizer-mediated laser irradiation for the photodynamic sterilization of blood transfusion products.

AB - The effect of Nd:YAG laser (30 mW, 532 nm) photoirradiation on the growth and colony formation of two strains of the Gram-negative bacteria Proteus mirabilis, the wild type S1959 and the mutant R45, differing in polysaccharide content in the bacterial cell wall lipopolysaccharide (LPS), was tested in the presence of the photosensitizer merocyanine 540 (MC540). Simultaneous action of MC540 and Nd:YAG laser light showed a differentiated influence depending on LPS structure: their bactericidal effect increased with depletion of the polysaccharide chain in LPS. Moreover, MC540 and laser illumination induced a dramatic increase in lipopolysaccharide toxicity, as monitored by means of the Limulus amebocyte lysate (LAL) test. This effect should be taken into account when applying combined photosensitizer-mediated laser irradiation for the photodynamic sterilization of blood transfusion products.

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Effects of Merocyanine 540-Sensitized Photoirradiation on Proteus mirabilis Growth and Lipopolysaccharide Toxicity

Abstract

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