Effects of human immunodeficiency virus type 1 infection on programmed cell death in the presence or absence of Bcl-2

In Woo Park, Eisaku Kondo, Louise Bergeron, Jinseu Park, Joseph Sodroski

Research output: Contribution to journalArticle

14 Scopus citations

Abstract

The effect of human immunodeficiency virus (HIV-1) infection on the programmed cell death of CD4 + lymphocytes was studied by using Jurkat cells stably expressing high levels of the Bcl-2 protein (Jurkat-Bcl2) or control cells (Jurkat-P). Both Jurkat-Bc12 and Jurkat-P cells exhibited surface CD4 expression adequate to support HIV-1 infection. We observed no differences between HIV-1-infected Jurkat Bcl2 cells and control cells with respect to kinetics of virus replication, protein expression, and processing. Severe cytopathic effects, which were typical of acute HIV-1 infection and consisted of syncytium formation followed by single-cell lysis, were observed in both cell types. However, several lines of evidence, such as cell viability analysis by trypan blue dye exclusion, chromosomal DNA laddering, and morphologic analysis by acridine orange/ethidium bromide or Giemsa staining, indicated that HIV-1 did not induce a significant amount of programmed cell death in either cell type. These results suggest that apoptosis is at most a minor element in HIV-1-induced cytopathicity in Jurkat lymphocytes.

Original languageEnglish
Pages (from-to)321-328
Number of pages8
JournalJournal of Acquired Immune Deficiency Syndromes and Human Retrovirology
Volume12
Issue number4
DOIs
StatePublished - 1996

Keywords

  • Apoptosis
  • Bcl-2
  • HIV-1
  • Jurkat cell
  • Programmed cell death

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