TY - JOUR
T1 - Effects of human immunodeficiency virus type 1 infection on programmed cell death in the presence or absence of Bcl-2
AU - Park, In Woo
AU - Kondo, Eisaku
AU - Bergeron, Louise
AU - Park, Jinseu
AU - Sodroski, Joseph
PY - 1996
Y1 - 1996
N2 - The effect of human immunodeficiency virus (HIV-1) infection on the programmed cell death of CD4 + lymphocytes was studied by using Jurkat cells stably expressing high levels of the Bcl-2 protein (Jurkat-Bcl2) or control cells (Jurkat-P). Both Jurkat-Bc12 and Jurkat-P cells exhibited surface CD4 expression adequate to support HIV-1 infection. We observed no differences between HIV-1-infected Jurkat Bcl2 cells and control cells with respect to kinetics of virus replication, protein expression, and processing. Severe cytopathic effects, which were typical of acute HIV-1 infection and consisted of syncytium formation followed by single-cell lysis, were observed in both cell types. However, several lines of evidence, such as cell viability analysis by trypan blue dye exclusion, chromosomal DNA laddering, and morphologic analysis by acridine orange/ethidium bromide or Giemsa staining, indicated that HIV-1 did not induce a significant amount of programmed cell death in either cell type. These results suggest that apoptosis is at most a minor element in HIV-1-induced cytopathicity in Jurkat lymphocytes.
AB - The effect of human immunodeficiency virus (HIV-1) infection on the programmed cell death of CD4 + lymphocytes was studied by using Jurkat cells stably expressing high levels of the Bcl-2 protein (Jurkat-Bcl2) or control cells (Jurkat-P). Both Jurkat-Bc12 and Jurkat-P cells exhibited surface CD4 expression adequate to support HIV-1 infection. We observed no differences between HIV-1-infected Jurkat Bcl2 cells and control cells with respect to kinetics of virus replication, protein expression, and processing. Severe cytopathic effects, which were typical of acute HIV-1 infection and consisted of syncytium formation followed by single-cell lysis, were observed in both cell types. However, several lines of evidence, such as cell viability analysis by trypan blue dye exclusion, chromosomal DNA laddering, and morphologic analysis by acridine orange/ethidium bromide or Giemsa staining, indicated that HIV-1 did not induce a significant amount of programmed cell death in either cell type. These results suggest that apoptosis is at most a minor element in HIV-1-induced cytopathicity in Jurkat lymphocytes.
KW - Apoptosis
KW - Bcl-2
KW - HIV-1
KW - Jurkat cell
KW - Programmed cell death
UR - http://www.scopus.com/inward/record.url?scp=9544230790&partnerID=8YFLogxK
U2 - 10.1097/00042560-199608010-00001
DO - 10.1097/00042560-199608010-00001
M3 - Article
C2 - 8673540
AN - SCOPUS:9544230790
SN - 1077-9450
VL - 12
SP - 321
EP - 328
JO - Journal of Acquired Immune Deficiency Syndromes and Human Retrovirology
JF - Journal of Acquired Immune Deficiency Syndromes and Human Retrovirology
IS - 4
ER -