[Effects of hsf1 genotype on the constitutive expression of alphaB-crystallin in mice myocardium].

L. Liu, Hong Hui Zhang, Guo Xian Ding, Yun Lin Cheng, Liang Jun Yan, I. J. Benjamin

Research output: Contribution to journalArticle

Abstract

Try to clarify the effects of HSF1 gene on the constitutively expressed alphaBC. To investigate the levels of constitutively expressed alphaB-Crystallin (alphaBC) in hsf1 knockout (hsf1 -/-) and hsf1 wild type (hsf1 +/+) mice myocardium by Western blot and immunohistochemistry. The alphaBC levels in hsf1 -/- and hsf1 +/+ were 68.42% +/- 4.16%, 100% +/- 7.58%, respectively (P < 0.05, cytosolic fraction), and 20.53% +/- 1.01%, 37.55% +/- 1.91%, respectively (P < 0.05, pellet fraction). The alphaBC signals decreased significantly in hsf1 -/- myocardium compared with hsf1 +/+ myocardium stained with fluorescence immunohistochemistry. hsf1 is the important, but not the only factor, which mediates the constitutively expressed alphaBC.

Original languageEnglish
Pages (from-to)30-33
Number of pages4
JournalZhongguo ying yong sheng li xue za zhi = Zhongguo yingyong shenglixue zazhi = Chinese journal of applied physiology
Volume20
Issue number1
StatePublished - Feb 2004

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