Effects of H-7 on the iris and ciliary muscle in monkeys

Baohe Tian, Cameron Millar, Paul L. Kaufman, Alexander Bershadsky, Eitan Becker, Benjamin Geiger

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24 Scopus citations

Abstract

Objectives: To determine the effects of H-7 on (1) iris and ciliary muscles (CMs) in living monkeys; (2) isolated monkey CM strips; (3) actomyosin contractility in cultured Swiss 3T3 cells. Methods: (1) Pupillary diameter (calipers) and accommodation (refractometer) in living monkeys were measured after topical, intracameral, or intravitreal administration of H-7 followed by systemic pilocarpine hydrochloride. (2) Pilocarpine-induced contraction of isolated monkey CM strips following administration of H-7 was measured in a perfusion chamber. (3) Actomyosin contractility in Swiss 3T3 cells cultured on thin silicone rubber film was determined by measuring cell- induced film wrinkles before and after administration of H-7. Results: Topical H-7 prevented anesthesia-induced miosis but did not affect resting refraction. Intracameral or intravitreal H-7 dilated the pupil and inhibited miotic but not accommodative responses to pilocarpine. H-7 inhibited pilocarpine-induced contraction of isolated monkey CM strips and reduced Swiss 3T3 cell contraction. Conclusions: H-7 inhibits actin-based contractility in non-muscle cells and in monkey iris sphincter and CM. Under our in vivo experimental conditions, the effect on the iris predominates over that on the CM.

Original languageEnglish
Pages (from-to)1070-1077
Number of pages8
JournalArchives of Ophthalmology
Volume116
Issue number8
DOIs
StatePublished - Aug 1998

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    Tian, B., Millar, C., Kaufman, P. L., Bershadsky, A., Becker, E., & Geiger, B. (1998). Effects of H-7 on the iris and ciliary muscle in monkeys. Archives of Ophthalmology, 116(8), 1070-1077. https://doi.org/10.1001/archopht.116.8.1070