TY - JOUR
T1 - Effects of H-7 on the iris and ciliary muscle in monkeys
AU - Tian, Baohe
AU - Millar, Cameron
AU - Kaufman, Paul L.
AU - Bershadsky, Alexander
AU - Becker, Eitan
AU - Geiger, Benjamin
PY - 1998/8
Y1 - 1998/8
N2 - Objectives: To determine the effects of H-7 on (1) iris and ciliary muscles (CMs) in living monkeys; (2) isolated monkey CM strips; (3) actomyosin contractility in cultured Swiss 3T3 cells. Methods: (1) Pupillary diameter (calipers) and accommodation (refractometer) in living monkeys were measured after topical, intracameral, or intravitreal administration of H-7 followed by systemic pilocarpine hydrochloride. (2) Pilocarpine-induced contraction of isolated monkey CM strips following administration of H-7 was measured in a perfusion chamber. (3) Actomyosin contractility in Swiss 3T3 cells cultured on thin silicone rubber film was determined by measuring cell- induced film wrinkles before and after administration of H-7. Results: Topical H-7 prevented anesthesia-induced miosis but did not affect resting refraction. Intracameral or intravitreal H-7 dilated the pupil and inhibited miotic but not accommodative responses to pilocarpine. H-7 inhibited pilocarpine-induced contraction of isolated monkey CM strips and reduced Swiss 3T3 cell contraction. Conclusions: H-7 inhibits actin-based contractility in non-muscle cells and in monkey iris sphincter and CM. Under our in vivo experimental conditions, the effect on the iris predominates over that on the CM.
AB - Objectives: To determine the effects of H-7 on (1) iris and ciliary muscles (CMs) in living monkeys; (2) isolated monkey CM strips; (3) actomyosin contractility in cultured Swiss 3T3 cells. Methods: (1) Pupillary diameter (calipers) and accommodation (refractometer) in living monkeys were measured after topical, intracameral, or intravitreal administration of H-7 followed by systemic pilocarpine hydrochloride. (2) Pilocarpine-induced contraction of isolated monkey CM strips following administration of H-7 was measured in a perfusion chamber. (3) Actomyosin contractility in Swiss 3T3 cells cultured on thin silicone rubber film was determined by measuring cell- induced film wrinkles before and after administration of H-7. Results: Topical H-7 prevented anesthesia-induced miosis but did not affect resting refraction. Intracameral or intravitreal H-7 dilated the pupil and inhibited miotic but not accommodative responses to pilocarpine. H-7 inhibited pilocarpine-induced contraction of isolated monkey CM strips and reduced Swiss 3T3 cell contraction. Conclusions: H-7 inhibits actin-based contractility in non-muscle cells and in monkey iris sphincter and CM. Under our in vivo experimental conditions, the effect on the iris predominates over that on the CM.
UR - http://www.scopus.com/inward/record.url?scp=0032454150&partnerID=8YFLogxK
U2 - 10.1001/archopht.116.8.1070
DO - 10.1001/archopht.116.8.1070
M3 - Article
C2 - 9715688
AN - SCOPUS:0032454150
SN - 0003-9950
VL - 116
SP - 1070
EP - 1077
JO - Archives of Ophthalmology
JF - Archives of Ophthalmology
IS - 8
ER -