TY - JOUR
T1 - Effects of fluorophore-to-silver distance on the emission of cyanine-dye-labeled oligonucleotides
AU - Malicka, Joanna
AU - Gryczynski, Ignacy
AU - Gryczynski, Zygmunt
AU - Lakowicz, Joseph R.
N1 - Funding Information:
This work was supported by the NIH National Center for Research Resources, RR-08119 and the National Institute of Biomedical Imaging and Bioengineering, EB000682. The authors thank Dr. J. Fang from the Center for Biomolecular Science and Engineering, Naval Research Laboratory, for the AFM measurements.
Copyright:
Copyright 2020 Elsevier B.V., All rights reserved.
PY - 2003/4/1
Y1 - 2003/4/1
N2 - We examined the fluorescence spectral properties of Cy3- and Cy5-labeled oligonucleotides at various distances from the surface of silver island films. The distance to the surface was controlled by alternating layers of biotinylated bovine serum albumin (BSA) and avidin, followed by binding of a biotinylated oligonucleotide. The maximum enhancement of fluorescence near a factor of 12 was observed for the first BSA-avidin layer, with the enhancement decreasing to 2-fold for six layers. The minimum lifetimes were observed for the first BSA-avidin layer, and were about 25-fold shorter than on quartz slides without silver, with the lifetimes being about 2-fold shorter for six BSA-avidin layers. These results suggest that maximum fluorescence enhancements occur about 90Å from the silver surface, a distance readily obtained by one or two layers of proteins.
AB - We examined the fluorescence spectral properties of Cy3- and Cy5-labeled oligonucleotides at various distances from the surface of silver island films. The distance to the surface was controlled by alternating layers of biotinylated bovine serum albumin (BSA) and avidin, followed by binding of a biotinylated oligonucleotide. The maximum enhancement of fluorescence near a factor of 12 was observed for the first BSA-avidin layer, with the enhancement decreasing to 2-fold for six layers. The minimum lifetimes were observed for the first BSA-avidin layer, and were about 25-fold shorter than on quartz slides without silver, with the lifetimes being about 2-fold shorter for six BSA-avidin layers. These results suggest that maximum fluorescence enhancements occur about 90Å from the silver surface, a distance readily obtained by one or two layers of proteins.
UR - http://www.scopus.com/inward/record.url?scp=0037379643&partnerID=8YFLogxK
U2 - 10.1016/S0003-2697(02)00702-9
DO - 10.1016/S0003-2697(02)00702-9
M3 - Article
C2 - 12672412
AN - SCOPUS:0037379643
SN - 0003-2697
VL - 315
SP - 57
EP - 66
JO - Analytical Biochemistry
JF - Analytical Biochemistry
IS - 1
ER -