TY - JOUR
T1 - Effects of exercise training on the autophagy-related muscular proteins expression in ovariectomized rats
AU - Zhong, Weiquan
AU - Shi, Xiangrong
AU - Yuan, Honghua
AU - Bu, Huimin
AU - Wu, Lianlian
AU - Wang, Renwei
N1 - Funding Information:
This study was supported by Shanghai Key Lab of Human Performance (Shanghai University of Sport) (No. 11DZ2261100) and Rehabilitation Medicine Brand Profession, Xuzhou Medical University.
Publisher Copyright:
© 2019 Zhong, Shi, Yuan, Bu, Wu and Wang.
PY - 2019
Y1 - 2019
N2 - Ovariectomy disrupts estrogen production and homeostasis. However, whether exercise training (ET) could counteract the ovariectomy-induced effect on muscular autophagy has remained elusive. This study examined muscular autophagy in ovariectomized (OVX) rats following 8 weeks of swimming ET. Here, 40 6-month-old female Sprague-Dawley rats were randomly divided into five groups: sham-operated control (Sham), OVX control (OVX), OVX with 60-min ET (OVX-60ET), 90-min ET (OVX-90ET), and 120-min ET (OVX-120ET) for 6 days/week. According to the results of Western blotting, the expression levels of autophagy-related proteins in the OVX gastrocnemius muscle, including mammalian target of rapamycin, uncoordinated 51-like kinase 1, Beclin-1, autophagy-related gene (Atg-7), and microtubule-Associated protein light chains 3 were significantly decreased (all P0.05), while there was an elevation on the p62 level. ET appreciably mitigated the OVX-induced negative effects on muscle quality and the autophagy pathway, which seemed to be dependent on ET volume. The most optimal outcomes were observed in the OVX-90ET group. The OVX-120 group had an adversely augmented catabolic process associated with gastrocnemius muscle atrophy. In conclusion, the expression levels of autophagy proteins are decreased in OVX rats, which can be appreciably mitigated following 8 weeks of swimming ET.
AB - Ovariectomy disrupts estrogen production and homeostasis. However, whether exercise training (ET) could counteract the ovariectomy-induced effect on muscular autophagy has remained elusive. This study examined muscular autophagy in ovariectomized (OVX) rats following 8 weeks of swimming ET. Here, 40 6-month-old female Sprague-Dawley rats were randomly divided into five groups: sham-operated control (Sham), OVX control (OVX), OVX with 60-min ET (OVX-60ET), 90-min ET (OVX-90ET), and 120-min ET (OVX-120ET) for 6 days/week. According to the results of Western blotting, the expression levels of autophagy-related proteins in the OVX gastrocnemius muscle, including mammalian target of rapamycin, uncoordinated 51-like kinase 1, Beclin-1, autophagy-related gene (Atg-7), and microtubule-Associated protein light chains 3 were significantly decreased (all P0.05), while there was an elevation on the p62 level. ET appreciably mitigated the OVX-induced negative effects on muscle quality and the autophagy pathway, which seemed to be dependent on ET volume. The most optimal outcomes were observed in the OVX-90ET group. The OVX-120 group had an adversely augmented catabolic process associated with gastrocnemius muscle atrophy. In conclusion, the expression levels of autophagy proteins are decreased in OVX rats, which can be appreciably mitigated following 8 weeks of swimming ET.
KW - Autophagy
KW - Body weight
KW - Ovariectomy
KW - Skeletal muscle
KW - Swimming exercise
UR - http://www.scopus.com/inward/record.url?scp=85069512488&partnerID=8YFLogxK
U2 - 10.3389/fphys.2019.00735
DO - 10.3389/fphys.2019.00735
M3 - Article
AN - SCOPUS:85069512488
SN - 1664-042X
VL - 10
JO - Frontiers in Physiology
JF - Frontiers in Physiology
IS - JUN
M1 - 735
ER -