Effects of exercise training on the autophagy-related muscular proteins expression in ovariectomized rats

Weiquan Zhong, Xiangrong Shi, Honghua Yuan, Huimin Bu, Lianlian Wu, Renwei Wang

Research output: Contribution to journalArticle

Abstract

Ovariectomy disrupts estrogen production and homeostasis. However, whether exercise training (ET) could counteract the ovariectomy-induced effect on muscular autophagy has remained elusive. This study examined muscular autophagy in ovariectomized (OVX) rats following 8 weeks of swimming ET. Here, 40 6-month-old female Sprague-Dawley rats were randomly divided into five groups: sham-operated control (Sham), OVX control (OVX), OVX with 60-min ET (OVX-60ET), 90-min ET (OVX-90ET), and 120-min ET (OVX-120ET) for 6 days/week. According to the results of Western blotting, the expression levels of autophagy-related proteins in the OVX gastrocnemius muscle, including mammalian target of rapamycin, uncoordinated 51-like kinase 1, Beclin-1, autophagy-related gene (Atg-7), and microtubule-Associated protein light chains 3 were significantly decreased (all P0.05), while there was an elevation on the p62 level. ET appreciably mitigated the OVX-induced negative effects on muscle quality and the autophagy pathway, which seemed to be dependent on ET volume. The most optimal outcomes were observed in the OVX-90ET group. The OVX-120 group had an adversely augmented catabolic process associated with gastrocnemius muscle atrophy. In conclusion, the expression levels of autophagy proteins are decreased in OVX rats, which can be appreciably mitigated following 8 weeks of swimming ET.

Original languageEnglish
Article number735
JournalFrontiers in Physiology
Volume10
Issue numberJUN
DOIs
StatePublished - 1 Jan 2019

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Exercise
Autophagy
Ovariectomy
Skeletal Muscle
Microtubule-Associated Proteins
Muscular Atrophy
Autophagy-Related Proteins
Sirolimus
Sprague Dawley Rats
Estrogens
Homeostasis
Phosphotransferases
Western Blotting
Light
Muscles
Genes
Proteins

Keywords

  • Autophagy
  • Body weight
  • Ovariectomy
  • Skeletal muscle
  • Swimming exercise

Cite this

Zhong, Weiquan ; Shi, Xiangrong ; Yuan, Honghua ; Bu, Huimin ; Wu, Lianlian ; Wang, Renwei. / Effects of exercise training on the autophagy-related muscular proteins expression in ovariectomized rats. In: Frontiers in Physiology. 2019 ; Vol. 10, No. JUN.
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Effects of exercise training on the autophagy-related muscular proteins expression in ovariectomized rats. / Zhong, Weiquan; Shi, Xiangrong; Yuan, Honghua; Bu, Huimin; Wu, Lianlian; Wang, Renwei.

In: Frontiers in Physiology, Vol. 10, No. JUN, 735, 01.01.2019.

Research output: Contribution to journalArticle

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