Abstract
We used resonance energy transfer and frequency-domain fluorometry to measure slow donor to acceptor diffusion in viscous media. The frequency-domain RET data were analyzed using a new numerical algorithm for predicting the donor intensity decay in the presence of diffusion occurring within the donor decay time. By the use of a rhenium metal-ligand complex as a microsecond decay time donor we were able to measure mutual donor-to-acceptor diffusion coefficients as low as 2×10-8 cm2/s. The availability of microsecond decay time luminophores and appropriate theory suggests the use of diffusion-enhanced energy transfer for measurement of diffusive processes and structural dynamics in biological systems.
Original language | English |
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Pages (from-to) | 661-668 |
Number of pages | 8 |
Journal | Chemical Physics Letters |
Volume | 319 |
Issue number | 5-6 |
DOIs | |
State | Published - 24 Mar 2000 |