Effects of diffusion on energy transfer in solution using a microsecond decay time rhenium metal-ligand complex as the donor

Józef Kuśba, Grzegorz Piszczek, Ignacy Gryczynski, Michael L. Johnson, Joseph R. Lakowicz

Research output: Contribution to journalArticlepeer-review

9 Scopus citations

Abstract

We used resonance energy transfer and frequency-domain fluorometry to measure slow donor to acceptor diffusion in viscous media. The frequency-domain RET data were analyzed using a new numerical algorithm for predicting the donor intensity decay in the presence of diffusion occurring within the donor decay time. By the use of a rhenium metal-ligand complex as a microsecond decay time donor we were able to measure mutual donor-to-acceptor diffusion coefficients as low as 2×10-8 cm2/s. The availability of microsecond decay time luminophores and appropriate theory suggests the use of diffusion-enhanced energy transfer for measurement of diffusive processes and structural dynamics in biological systems.

Original languageEnglish
Pages (from-to)661-668
Number of pages8
JournalChemical Physics Letters
Volume319
Issue number5-6
DOIs
StatePublished - 24 Mar 2000

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