Effects of diffusion on energy transfer in solution using a microsecond decay time rhenium metal-ligand complex as the donor

Józef Kuśba; Grzegorz Piszczek; Ignacy Gryczynski; Michael L. Johnson; Joseph R. Lakowicz

doi:10.1016/S0009-2614(00)00071-3

Effects of diffusion on energy transfer in solution using a microsecond decay time rhenium metal-ligand complex as the donor

Józef Kuśba, Grzegorz Piszczek, Ignacy Gryczynski, Michael L. Johnson, Joseph R. Lakowicz

Research output: Contribution to journal › Article › peer-review

9 Scopus citations

Abstract

We used resonance energy transfer and frequency-domain fluorometry to measure slow donor to acceptor diffusion in viscous media. The frequency-domain RET data were analyzed using a new numerical algorithm for predicting the donor intensity decay in the presence of diffusion occurring within the donor decay time. By the use of a rhenium metal-ligand complex as a microsecond decay time donor we were able to measure mutual donor-to-acceptor diffusion coefficients as low as 2×10^-8 cm²/s. The availability of microsecond decay time luminophores and appropriate theory suggests the use of diffusion-enhanced energy transfer for measurement of diffusive processes and structural dynamics in biological systems.

Original language	English
Pages (from-to)	661-668
Number of pages	8
Journal	Chemical Physics Letters
Volume	319
Issue number	5-6
DOIs	https://doi.org/10.1016/S0009-2614(00)00071-3
State	Published - 24 Mar 2000

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title = "Effects of diffusion on energy transfer in solution using a microsecond decay time rhenium metal-ligand complex as the donor",

abstract = "We used resonance energy transfer and frequency-domain fluorometry to measure slow donor to acceptor diffusion in viscous media. The frequency-domain RET data were analyzed using a new numerical algorithm for predicting the donor intensity decay in the presence of diffusion occurring within the donor decay time. By the use of a rhenium metal-ligand complex as a microsecond decay time donor we were able to measure mutual donor-to-acceptor diffusion coefficients as low as 2×10-8 cm2/s. The availability of microsecond decay time luminophores and appropriate theory suggests the use of diffusion-enhanced energy transfer for measurement of diffusive processes and structural dynamics in biological systems.",

author = "J{\'o}zef Ku{\'s}ba and Grzegorz Piszczek and Ignacy Gryczynski and Johnson, {Michael L.} and Lakowicz, {Joseph R.}",

note = "Funding Information: This work was supported by grants from the National Institutes of Health, GM-35154 and RR-08119. ",

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T1 - Effects of diffusion on energy transfer in solution using a microsecond decay time rhenium metal-ligand complex as the donor

AU - Kuśba, Józef

AU - Piszczek, Grzegorz

AU - Gryczynski, Ignacy

AU - Johnson, Michael L.

AU - Lakowicz, Joseph R.

N1 - Funding Information: This work was supported by grants from the National Institutes of Health, GM-35154 and RR-08119.

PY - 2000/3/24

Y1 - 2000/3/24

N2 - We used resonance energy transfer and frequency-domain fluorometry to measure slow donor to acceptor diffusion in viscous media. The frequency-domain RET data were analyzed using a new numerical algorithm for predicting the donor intensity decay in the presence of diffusion occurring within the donor decay time. By the use of a rhenium metal-ligand complex as a microsecond decay time donor we were able to measure mutual donor-to-acceptor diffusion coefficients as low as 2×10-8 cm2/s. The availability of microsecond decay time luminophores and appropriate theory suggests the use of diffusion-enhanced energy transfer for measurement of diffusive processes and structural dynamics in biological systems.

AB - We used resonance energy transfer and frequency-domain fluorometry to measure slow donor to acceptor diffusion in viscous media. The frequency-domain RET data were analyzed using a new numerical algorithm for predicting the donor intensity decay in the presence of diffusion occurring within the donor decay time. By the use of a rhenium metal-ligand complex as a microsecond decay time donor we were able to measure mutual donor-to-acceptor diffusion coefficients as low as 2×10-8 cm2/s. The availability of microsecond decay time luminophores and appropriate theory suggests the use of diffusion-enhanced energy transfer for measurement of diffusive processes and structural dynamics in biological systems.

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U2 - 10.1016/S0009-2614(00)00071-3

DO - 10.1016/S0009-2614(00)00071-3

M3 - Article

AN - SCOPUS:0008645441

SN - 0009-2614

VL - 319

SP - 661

EP - 668

JO - Chemical Physics Letters

JF - Chemical Physics Letters

IS - 5-6

ER -

Effects of diffusion on energy transfer in solution using a microsecond decay time rhenium metal-ligand complex as the donor

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