Effects of detergents on the lecithin: Cholesterol acyltransferase reaction

Myung C. Park, Walter J. McConathy, Andras G. Lacko

Research output: Contribution to journalArticle

4 Citations (Scopus)

Abstract

This paper describes the effect of an ionic (sodium dodecyl sulfate; SDS) and a nonionic detergent (Triton X-100) on the substrate and enzyme components of the lecithin: cholesterol acyltransferase (LCAT) reaction. When the enzyme sources (purified or partially purified) or the respective substrates [high-density lipoproteins (HDL) or proteoliposomes] were preincubated with detergents, a consistent trend in LCAT activity was only seen when partially purified LCAT was used as the enzyme source. This trend indicated an approximately 25% increase in enzyme activity over the control when 10-4, m SDS and 2 × 10-3 % Triton X-100 were present in the preincubation mixtures, respectively. Those observations suggested that, during the preincubations and subsequent assays, the enzyme (in the presence of detergents) was allowed to dissociate from the endogenous substrate and subsequently interact with the exogenous substrate molecules. Additional experiments utilizing molecular-sieve chromatography with whole plasma and partially purified enzyme also showed that dissociation of LCAT/lipoprotein complexes occurred in the presence of detergent. SDS was also shown to enhance the reaction of LCAT in whole plasma with anti-LCAT antibody in an enzyme-linked immunoassay system, indicating that the detergent treatment facilitated the exposure of additional antigenic sites, perhaps via dissociation of the enzyme from plasma lipoproteins.

Original languageEnglish
Pages (from-to)290-298
Number of pages9
JournalArchives of Biochemistry and Biophysics
Volume233
Issue number1
DOIs
StatePublished - 15 Aug 1984

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Phosphatidylcholine-Sterol O-Acyltransferase
Detergents
Enzymes
Octoxynol
Lipoproteins
Substrates
Plasmas
Enzyme Assays
HDL Lipoproteins
Immunoenzyme Techniques
Sodium Dodecyl Sulfate
Molecular sieves
Enzyme activity
Gel Chromatography
Chromatography
Assays
Antibodies
Molecules

Cite this

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title = "Effects of detergents on the lecithin: Cholesterol acyltransferase reaction",
abstract = "This paper describes the effect of an ionic (sodium dodecyl sulfate; SDS) and a nonionic detergent (Triton X-100) on the substrate and enzyme components of the lecithin: cholesterol acyltransferase (LCAT) reaction. When the enzyme sources (purified or partially purified) or the respective substrates [high-density lipoproteins (HDL) or proteoliposomes] were preincubated with detergents, a consistent trend in LCAT activity was only seen when partially purified LCAT was used as the enzyme source. This trend indicated an approximately 25{\%} increase in enzyme activity over the control when 10-4, m SDS and 2 × 10-3 {\%} Triton X-100 were present in the preincubation mixtures, respectively. Those observations suggested that, during the preincubations and subsequent assays, the enzyme (in the presence of detergents) was allowed to dissociate from the endogenous substrate and subsequently interact with the exogenous substrate molecules. Additional experiments utilizing molecular-sieve chromatography with whole plasma and partially purified enzyme also showed that dissociation of LCAT/lipoprotein complexes occurred in the presence of detergent. SDS was also shown to enhance the reaction of LCAT in whole plasma with anti-LCAT antibody in an enzyme-linked immunoassay system, indicating that the detergent treatment facilitated the exposure of additional antigenic sites, perhaps via dissociation of the enzyme from plasma lipoproteins.",
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Effects of detergents on the lecithin : Cholesterol acyltransferase reaction. / Park, Myung C.; McConathy, Walter J.; Lacko, Andras G.

In: Archives of Biochemistry and Biophysics, Vol. 233, No. 1, 15.08.1984, p. 290-298.

Research output: Contribution to journalArticle

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