TY - JOUR
T1 - Effect of the spin-trapping compound N-tert-butyl-α-phenylnitrone on protein oxidation and life span
AU - Dubey, Anju
AU - Forster, Michael J.
AU - Sohal, Rajindar S.
N1 - Funding Information:
We are grateful to Dr. Sanjiv Agarwal for his insightful scienti®c input in the preparation of the manuscript. We are thankful to Ms. Kim Dawson for providing technical assistance. This research was supported by Grants RO1AG7657 and RO1AG7695 from the National Institutes of Health±National Institute on Aging.
PY - 1995/12
Y1 - 1995/12
N2 - N-tert-Butyl-α-phenylnitrone (PBN), a lipophilic spin-trapping compound, has been reported to decrease the concentration of protein carbonyls, the products of protein oxidation, in the brain of old gerbils to virtually the level found in the young gerbils (Carney, J. M., et al. (1991) Proc. Natl. Acad. Sci. USA 88, 3633-3636). The validity of this finding as well as that of the commonly used 2,4-dinitrophenylhydrazine procedure for the measurement of protein carbonyls was recently called into question by Cao and Cutler ((1995) Arch. Biochem. Biophys. 320, 106-114). The objective of the present study was to examine some of the relevant issues such as (a) whether the original findings on the effects of PBN can be confirmed, (b) whether similar effects of PBN occur in other species and tissues, and (c) whether PBN affects the life span of animals. Results of this study provide confirmation of the original finding that PBN indeed causes a decrease in protein carbonyl content in the gerbil brain cortex. However, a similar effect is not observed in the gerbil heart or the mouse brain cortex. Effects of PBN on protein carbonyls are thus variable depending upon tissue and species. PBN administration did not extend the life span of houseflies and at relatively high concentrations it was found to be toxic.
AB - N-tert-Butyl-α-phenylnitrone (PBN), a lipophilic spin-trapping compound, has been reported to decrease the concentration of protein carbonyls, the products of protein oxidation, in the brain of old gerbils to virtually the level found in the young gerbils (Carney, J. M., et al. (1991) Proc. Natl. Acad. Sci. USA 88, 3633-3636). The validity of this finding as well as that of the commonly used 2,4-dinitrophenylhydrazine procedure for the measurement of protein carbonyls was recently called into question by Cao and Cutler ((1995) Arch. Biochem. Biophys. 320, 106-114). The objective of the present study was to examine some of the relevant issues such as (a) whether the original findings on the effects of PBN can be confirmed, (b) whether similar effects of PBN occur in other species and tissues, and (c) whether PBN affects the life span of animals. Results of this study provide confirmation of the original finding that PBN indeed causes a decrease in protein carbonyl content in the gerbil brain cortex. However, a similar effect is not observed in the gerbil heart or the mouse brain cortex. Effects of PBN on protein carbonyls are thus variable depending upon tissue and species. PBN administration did not extend the life span of houseflies and at relatively high concentrations it was found to be toxic.
KW - Aging
KW - Free radicals
KW - Life span
KW - N-tert-butyl-α-phenylnitrone
KW - Oxidative stress
KW - Protein oxidation
KW - Reactive oxygen species
UR - http://www.scopus.com/inward/record.url?scp=0029561638&partnerID=8YFLogxK
U2 - 10.1006/abbi.1995.0037
DO - 10.1006/abbi.1995.0037
M3 - Article
C2 - 8554316
AN - SCOPUS:0029561638
SN - 0003-9861
VL - 324
SP - 249
EP - 254
JO - Archives of Biochemistry and Biophysics
JF - Archives of Biochemistry and Biophysics
IS - 2
ER -