Effect of temperature during assembly on the structure and mechanical properties of peptide-based materials

Sivakumar Ramachandran; Marc B. Taraban; Jill Trewhella; Ignacy Gryczynski; Zygmunt Gryczynski; Yihua Bruce Yu

doi:10.1021/bm100138m

Effect of temperature during assembly on the structure and mechanical properties of peptide-based materials

Sivakumar Ramachandran, Marc B. Taraban, Jill Trewhella, Ignacy Gryczynski, Zygmunt Gryczynski, Yihua Bruce Yu

Microbiology, Immunology & Genetics

Research output: Contribution to journal › Article › peer-review

22 Scopus citations

Abstract

Mutually complementary, self-repulsive oligopeptide pairs were designed to coassemble into viscoelastic hydrogels. Peptide engineering was combined with biophysical techniques to investigate the effects of temperature on the structural and mechanical properties of the resulting hydrogels. Biophysical characterizations, including dynamic rheometry, small-angle X-ray scattering (SAXS), and fluorescence spectroscopy, were used to investigate hydrogelation at the bulk, fiber, and molecular levels, respectively. It has been found that temperature has a significant effect on the structure and mechanical properties of peptide-based biomaterials. Oligopeptide fibers assembled at 25 °C are formed faster and are two times thicker, and the resulting material is mechanically seven times stronger than that assembled at 5 °C.

Original language	English
Pages (from-to)	1502-1506
Number of pages	5
Journal	Biomacromolecules
Volume	11
Issue number	6
DOIs	https://doi.org/10.1021/bm100138m
State	Published - 14 Jul 2010

Access to Document

10.1021/bm100138m

Cite this

@article{3ff1d94d502d43dbb30144775869b7ea,

title = "Effect of temperature during assembly on the structure and mechanical properties of peptide-based materials",

abstract = "Mutually complementary, self-repulsive oligopeptide pairs were designed to coassemble into viscoelastic hydrogels. Peptide engineering was combined with biophysical techniques to investigate the effects of temperature on the structural and mechanical properties of the resulting hydrogels. Biophysical characterizations, including dynamic rheometry, small-angle X-ray scattering (SAXS), and fluorescence spectroscopy, were used to investigate hydrogelation at the bulk, fiber, and molecular levels, respectively. It has been found that temperature has a significant effect on the structure and mechanical properties of peptide-based biomaterials. Oligopeptide fibers assembled at 25 °C are formed faster and are two times thicker, and the resulting material is mechanically seven times stronger than that assembled at 5 °C.",

author = "Sivakumar Ramachandran and Taraban, {Marc B.} and Jill Trewhella and Ignacy Gryczynski and Zygmunt Gryczynski and Yu, {Yihua Bruce}",

year = "2010",

month = jul,

day = "14",

doi = "10.1021/bm100138m",

language = "English",

volume = "11",

pages = "1502--1506",

journal = "Biomacromolecules",

issn = "1525-7797",

publisher = "American Chemical Society",

number = "6",

}

TY - JOUR

T1 - Effect of temperature during assembly on the structure and mechanical properties of peptide-based materials

AU - Ramachandran, Sivakumar

AU - Taraban, Marc B.

AU - Trewhella, Jill

AU - Gryczynski, Ignacy

AU - Gryczynski, Zygmunt

AU - Yu, Yihua Bruce

PY - 2010/7/14

Y1 - 2010/7/14

N2 - Mutually complementary, self-repulsive oligopeptide pairs were designed to coassemble into viscoelastic hydrogels. Peptide engineering was combined with biophysical techniques to investigate the effects of temperature on the structural and mechanical properties of the resulting hydrogels. Biophysical characterizations, including dynamic rheometry, small-angle X-ray scattering (SAXS), and fluorescence spectroscopy, were used to investigate hydrogelation at the bulk, fiber, and molecular levels, respectively. It has been found that temperature has a significant effect on the structure and mechanical properties of peptide-based biomaterials. Oligopeptide fibers assembled at 25 °C are formed faster and are two times thicker, and the resulting material is mechanically seven times stronger than that assembled at 5 °C.

AB - Mutually complementary, self-repulsive oligopeptide pairs were designed to coassemble into viscoelastic hydrogels. Peptide engineering was combined with biophysical techniques to investigate the effects of temperature on the structural and mechanical properties of the resulting hydrogels. Biophysical characterizations, including dynamic rheometry, small-angle X-ray scattering (SAXS), and fluorescence spectroscopy, were used to investigate hydrogelation at the bulk, fiber, and molecular levels, respectively. It has been found that temperature has a significant effect on the structure and mechanical properties of peptide-based biomaterials. Oligopeptide fibers assembled at 25 °C are formed faster and are two times thicker, and the resulting material is mechanically seven times stronger than that assembled at 5 °C.

UR - http://www.scopus.com/inward/record.url?scp=77954580602&partnerID=8YFLogxK

U2 - 10.1021/bm100138m

DO - 10.1021/bm100138m

M3 - Article

C2 - 20481580

AN - SCOPUS:77954580602

SN - 1525-7797

VL - 11

SP - 1502

EP - 1506

JO - Biomacromolecules

JF - Biomacromolecules

IS - 6

ER -

Effect of temperature during assembly on the structure and mechanical properties of peptide-based materials

Abstract

Access to Document

Other files and links

Fingerprint

Cite this