Effect of storage conditions on restriction fragment length polymorphism (RFLP) analysis of deoxyribonucleic acid (DNA) bound to positively charged nylon membranes

A. M. Giusti, Bruce Budowle

Research output: Contribution to journalArticle

6 Scopus citations

Abstract

The ability to generate an autoradiogram from deoxyribonucleic acid (DNA) immobilized on a positively charged nylon membrane could be compromised by the storage conditions of the membrane. HaeIII-digested human DNA was size fractionated and transferred to two types of positively charged nylon membranes. The membranes were stored at -20°C, 4°C, and ambient temperature and humidity for times ranging from 1 day to 13 weeks, then hybridized to variable number of tandem repeat (VNTR) probes to examine the effect of the storage conditions on the membrane-bound DNAs. It was shown that such membranes could be successfully hybridized and rehybridized if they were stored at -20 or 4°C, but storage under ambient conditions reduced or eliminated the likelihood of successful hybridization.

Original languageEnglish
Pages (from-to)597-603
Number of pages7
JournalJournal of Forensic Sciences
Volume37
Issue number2
StatePublished - 1 Jan 1992

Keywords

  • deoxyribonucleic acid (DNA)
  • hybridization
  • nylon membranes
  • pathology and biology
  • restriction fragment length polymorphisms
  • storage conditions
  • variable number of tandem repeats

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