Abstract
The ability to generate an autoradiogram from deoxyribonucleic acid (DNA) immobilized on a positively charged nylon membrane could be compromised by the storage conditions of the membrane. HaeIII-digested human DNA was size fractionated and transferred to two types of positively charged nylon membranes. The membranes were stored at -20°C, 4°C, and ambient temperature and humidity for times ranging from 1 day to 13 weeks, then hybridized to variable number of tandem repeat (VNTR) probes to examine the effect of the storage conditions on the membrane-bound DNAs. It was shown that such membranes could be successfully hybridized and rehybridized if they were stored at -20 or 4°C, but storage under ambient conditions reduced or eliminated the likelihood of successful hybridization.
Original language | English |
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Pages (from-to) | 597-603 |
Number of pages | 7 |
Journal | Journal of Forensic Sciences |
Volume | 37 |
Issue number | 2 |
State | Published - 1 Jan 1992 |
Keywords
- deoxyribonucleic acid (DNA)
- hybridization
- nylon membranes
- pathology and biology
- restriction fragment length polymorphisms
- storage conditions
- variable number of tandem repeats