Effect of regulated expression of the fragile histidine triad gene on cell cycle and proliferation

Research output: Contribution to journalArticle

18 Citations (Scopus)

Abstract

The mechanism of tumor suppressor action of the fragile histidine triad (FHIT) gene is unknown. Disruption of cell cycle regulation leads to the tumor formation and many tumor suppressor genes suppress tumorigenesis through their effect on cell cycle regulation. We examined the expression of FHIT during the cell cycle, and determined whether overexpression of FHIT affects cell cycle kinetics and apoptosis. The FHIT cDNA was cloned into the ecdysone-inducible expression vector in both the sense and antisense orientations. Overexpression of the sense or antisense construct did not affect cell proliferation, cell cycle distribution or apoptosis in human 293T cells. Analysis of the FHIT expression in 293T cells collected at various cell cycle phases showed that the expression of FHIT is not under cell cycle regulation. These results indicate that the tumor suppressor activity of the FHIT gene may be independent of an effect on the cell cycle and apoptosis mechanisms.

Original languageEnglish
Pages (from-to)83-88
Number of pages6
JournalMolecular and Cellular Biochemistry
Volume204
Issue number1-2
StatePublished - 6 Mar 2000

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Histidine
Cell Cycle
Genes
Cells
Cell Proliferation
Tumors
HEK293 Cells
Apoptosis
Ecdysone
Neoplasms
Cell proliferation
Tumor Suppressor Genes
Carcinogenesis
Complementary DNA
Kinetics

Keywords

  • Apoptosis
  • Cell cycle
  • Ecdysone
  • FHIT
  • Tumor suppressor

Cite this

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abstract = "The mechanism of tumor suppressor action of the fragile histidine triad (FHIT) gene is unknown. Disruption of cell cycle regulation leads to the tumor formation and many tumor suppressor genes suppress tumorigenesis through their effect on cell cycle regulation. We examined the expression of FHIT during the cell cycle, and determined whether overexpression of FHIT affects cell cycle kinetics and apoptosis. The FHIT cDNA was cloned into the ecdysone-inducible expression vector in both the sense and antisense orientations. Overexpression of the sense or antisense construct did not affect cell proliferation, cell cycle distribution or apoptosis in human 293T cells. Analysis of the FHIT expression in 293T cells collected at various cell cycle phases showed that the expression of FHIT is not under cell cycle regulation. These results indicate that the tumor suppressor activity of the FHIT gene may be independent of an effect on the cell cycle and apoptosis mechanisms.",
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Effect of regulated expression of the fragile histidine triad gene on cell cycle and proliferation. / Guo, Zongyou; Vishwanatha, Jamboor K.

In: Molecular and Cellular Biochemistry, Vol. 204, No. 1-2, 06.03.2000, p. 83-88.

Research output: Contribution to journalArticle

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AB - The mechanism of tumor suppressor action of the fragile histidine triad (FHIT) gene is unknown. Disruption of cell cycle regulation leads to the tumor formation and many tumor suppressor genes suppress tumorigenesis through their effect on cell cycle regulation. We examined the expression of FHIT during the cell cycle, and determined whether overexpression of FHIT affects cell cycle kinetics and apoptosis. The FHIT cDNA was cloned into the ecdysone-inducible expression vector in both the sense and antisense orientations. Overexpression of the sense or antisense construct did not affect cell proliferation, cell cycle distribution or apoptosis in human 293T cells. Analysis of the FHIT expression in 293T cells collected at various cell cycle phases showed that the expression of FHIT is not under cell cycle regulation. These results indicate that the tumor suppressor activity of the FHIT gene may be independent of an effect on the cell cycle and apoptosis mechanisms.

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