Effect of quencher, denaturants, temperature and pH on the fluorescent properties of BSA protected gold nanoclusters

Rahul Chib, Susan Butler, Sangram Raut, Sunil Shah, Julian Borejdo, Zygmunt Gryczynski, Ignacy Gryczynski

Research output: Contribution to journalArticle

16 Scopus citations

Abstract

In this paper, we have synthesized BSA protected gold nanoclusters (BSA Au nanocluster) and studied the effect of quencher, protein denaturant, pH and temperature on the fluorescence properties of the tryptophan molecule of the BSA Au nanocluster and native BSA. We have also studied their effect on the peak emission of BSA Au nanoclusters (650 nm). The photophysical characterization of a newly developed fluorophore in different environments is absolutely necessary to futher develop their biomedical and analytical applications. It was observed from our experiments that the tryptophan in BSA Au nanoclusters is better shielded from the polar environment. Tryptophan in native BSA showed a red shift in its peak emission wavelength position. Tryptophan is a highly polarity sensitive dye and a minimal change in its microenvironment can be easily observed in its photophysical properties.

Original languageEnglish
Article numberLUMIND1500227
Pages (from-to)62-68
Number of pages7
JournalJournal of Luminescence
Volume168
DOIs
StatePublished - 17 Aug 2015

Keywords

  • BSA Au nanoclusters
  • Denaturants
  • Fluorescence
  • Quenching
  • Tryptophan emission

Fingerprint Dive into the research topics of 'Effect of quencher, denaturants, temperature and pH on the fluorescent properties of BSA protected gold nanoclusters'. Together they form a unique fingerprint.

  • Cite this