Effect of quencher, denaturants, temperature and pH on the fluorescent properties of BSA protected gold nanoclusters

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9 Citations (Scopus)

Abstract

In this paper, we have synthesized BSA protected gold nanoclusters (BSA Au nanocluster) and studied the effect of quencher, protein denaturant, pH and temperature on the fluorescence properties of the tryptophan molecule of the BSA Au nanocluster and native BSA. We have also studied their effect on the peak emission of BSA Au nanoclusters (650 nm). The photophysical characterization of a newly developed fluorophore in different environments is absolutely necessary to futher develop their biomedical and analytical applications. It was observed from our experiments that the tryptophan in BSA Au nanoclusters is better shielded from the polar environment. Tryptophan in native BSA showed a red shift in its peak emission wavelength position. Tryptophan is a highly polarity sensitive dye and a minimal change in its microenvironment can be easily observed in its photophysical properties.

Original languageEnglish
Article numberLUMIND1500227
Pages (from-to)62-68
Number of pages7
JournalJournal of Luminescence
Volume168
DOIs
StatePublished - 17 Aug 2015

Fingerprint

Nanoclusters
nanoclusters
Tryptophan
Gold
tryptophan
gold
Temperature
temperature
Fluorophores
Coloring Agents
red shift
Fluorescence
polarity
dyes
proteins
Wavelength
fluorescence
Molecules
Proteins
wavelengths

Keywords

  • BSA Au nanoclusters
  • Denaturants
  • Fluorescence
  • Quenching
  • Tryptophan emission

Cite this

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title = "Effect of quencher, denaturants, temperature and pH on the fluorescent properties of BSA protected gold nanoclusters",
abstract = "In this paper, we have synthesized BSA protected gold nanoclusters (BSA Au nanocluster) and studied the effect of quencher, protein denaturant, pH and temperature on the fluorescence properties of the tryptophan molecule of the BSA Au nanocluster and native BSA. We have also studied their effect on the peak emission of BSA Au nanoclusters (650 nm). The photophysical characterization of a newly developed fluorophore in different environments is absolutely necessary to futher develop their biomedical and analytical applications. It was observed from our experiments that the tryptophan in BSA Au nanoclusters is better shielded from the polar environment. Tryptophan in native BSA showed a red shift in its peak emission wavelength position. Tryptophan is a highly polarity sensitive dye and a minimal change in its microenvironment can be easily observed in its photophysical properties.",
keywords = "BSA Au nanoclusters, Denaturants, Fluorescence, Quenching, Tryptophan emission",
author = "Rahul Chib and Susan Butler and Raut, {Sangram Limbaji} and Sunil Shah and Julian Borejdo and Zygmunt Gryczynski and Ignacy Gryczynski",
year = "2015",
month = "8",
day = "17",
doi = "10.1016/j.jlumin.2015.07.030",
language = "English",
volume = "168",
pages = "62--68",
journal = "Journal of Luminescence",
issn = "0022-2313",
publisher = "Elsevier",

}

Effect of quencher, denaturants, temperature and pH on the fluorescent properties of BSA protected gold nanoclusters. / Chib, Rahul; Butler, Susan; Raut, Sangram Limbaji; Shah, Sunil; Borejdo, Julian; Gryczynski, Zygmunt; Gryczynski, Ignacy.

In: Journal of Luminescence, Vol. 168, LUMIND1500227, 17.08.2015, p. 62-68.

Research output: Contribution to journalArticleResearchpeer-review

TY - JOUR

T1 - Effect of quencher, denaturants, temperature and pH on the fluorescent properties of BSA protected gold nanoclusters

AU - Chib, Rahul

AU - Butler, Susan

AU - Raut, Sangram Limbaji

AU - Shah, Sunil

AU - Borejdo, Julian

AU - Gryczynski, Zygmunt

AU - Gryczynski, Ignacy

PY - 2015/8/17

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AB - In this paper, we have synthesized BSA protected gold nanoclusters (BSA Au nanocluster) and studied the effect of quencher, protein denaturant, pH and temperature on the fluorescence properties of the tryptophan molecule of the BSA Au nanocluster and native BSA. We have also studied their effect on the peak emission of BSA Au nanoclusters (650 nm). The photophysical characterization of a newly developed fluorophore in different environments is absolutely necessary to futher develop their biomedical and analytical applications. It was observed from our experiments that the tryptophan in BSA Au nanoclusters is better shielded from the polar environment. Tryptophan in native BSA showed a red shift in its peak emission wavelength position. Tryptophan is a highly polarity sensitive dye and a minimal change in its microenvironment can be easily observed in its photophysical properties.

KW - BSA Au nanoclusters

KW - Denaturants

KW - Fluorescence

KW - Quenching

KW - Tryptophan emission

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