Effect of neural precursor proliferation level on neurogenesis in rat brain during aging and after focal ischemia

Huidong Tang, Yaoming Wang, Lin Xie, Xiao Ou Mao, Seok Joon Won, Veronica Galvan, Kunlin Jin

Research output: Contribution to journalArticle

23 Scopus citations

Abstract

The observed age-related decline in neurogenesis may result from reduced proliferation or increased death rate of neuronal precursor cells (NPCs). We found that caspase-3, but not caspase-6, -7, or -9, was activated in NPCs in neurogenic regions of young, young-adult, middle-aged and aged rat brains. The number of capase-3-immunoreactive cells was highest in young and lowest in aged rats. Surprisingly, intraventricular administration of a caspase-3 inhibitor failed to restore the number of BrdU-positive cells in the aged dentate gyrus, suggesting that the age-related decline in neurogenesis may be attributable primarily to reduced proliferation. Additionally, we also found that NPCs in the subventricular zone of young-adult and aged rat brain were increased after focal cerebral ischemia, suggesting that the increase in neurogenesis induced by ischemia may result from an increase in the rate of NPC proliferation, but not from a decrease in NPC death. Thus, our results suggest that age-related and injury-induced changes in the rate of neurogenesis are controlled at the level of NPC proliferation. Furthermore, our results may imply that the mechanisms that maintain a stable population of NPCs in the normal adult and in the ischemic brain, which account for the observed age-dependent reduction or injury-induced increases in neurogenesis, impinge on the regulation of cell division at the NPC level.

Original languageEnglish
Pages (from-to)299-308
Number of pages10
JournalNeurobiology of Aging
Volume30
Issue number2
DOIs
StatePublished - Feb 2009

Keywords

  • Aging
  • Cell death
  • Dentate gyrus
  • Ischemia
  • Neurogenesis
  • Subventricular zone

Fingerprint Dive into the research topics of 'Effect of neural precursor proliferation level on neurogenesis in rat brain during aging and after focal ischemia'. Together they form a unique fingerprint.

  • Cite this