Effect of Na+ on intestinal succinate transport and metabolism in vitro

A. J. Moe, R. T. Mallet, M. J. Jackson, J. A. Hollywood, J. K. Kelleher

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Abstract

The effect of Na+ on 14CO2 production from [14C]succinate was studied in isolated rat enterocytes, and Na+-dependent succinate transport was characterized in pig intestinal brush-border membrane vesicles. The production of 14CO2 from [14C]succinate by enterocytes was decreased 12-fold when Na+ was replaced by N-methyl-D-glucamine in the absence of glutamine and 20-fold in the presence of 0.2 or 0.5 mM glutamine. The ratio of 14CO2 produced from [1,4-14C]succinate to that produced by [2,3-14C]succinate was not affected by Na+ replacement, indicating that the pattern of tricarboxylic acid cycle metabolism was not altered. The uptake of [14C]succinate by brush-border membrane vesicles was stimulated 10-fold in the presence of 100 mM NaCl compared with 100 mM KCl. When succinate uptake was correlated to transport into an osmotically sensitive space, the magnitude of the Na+ stimulation was 20-fold. Succinate transport into brush-border membrane vesicles was Na+ dependent, electroneutral, nonconcentrative, with an apparent Na+-succinate coupling ratio of 2:1. Results of this study indicate that Na+-stimulated CO2 production by enterocytes can be explained by the effect of Na+ on succinate transport across the brush-border membrane.

Original languageEnglish
Pages (from-to)24/1
JournalAmerican Journal of Physiology - Cell Physiology
Volume255
Issue number1
StatePublished - 1988

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    Moe, A. J., Mallet, R. T., Jackson, M. J., Hollywood, J. A., & Kelleher, J. K. (1988). Effect of Na+ on intestinal succinate transport and metabolism in vitro. American Journal of Physiology - Cell Physiology, 255(1), 24/1.