Effect of molecular ordering on distance distributions of flexible donor-acceptor pairs

Ignacy Gryczynski, Zygmunt Gryczynski, Wieslaw M. Wiczk, Jozef Kusba, Joseph R. Lakowicz

Research output: Chapter in Book/Report/Conference proceedingConference contributionResearchpeer-review

7 Citations (Scopus)

Abstract

Measurements of time-resolved intramolecular energy transfer in progressively stretched poly(vinyl alcohol) films were performed. The donor (tryptophan) and acceptor (dansyl) were linked with flexible polymethylene chain. Distance distributions were recovered from frequency-domain measurements of the donor decay. In isotropic PVA (in solution) a wide range of distances were detected (Gaussian full width at half maximum of about 16 angstroms) with an average distance of 13 angstroms. The donor-acceptor distance distribution became progressively more narrow when the PVA films were stretched. Four-fold or more stretching results in a single donor-acceptor distance. The maximal measured donor-acceptor distance of 23 angstroms is in excellent agreement with the computed distance for the fully stretched conformation. The possibility of partial and/or full ordering of polymethylene chains by stretching the PVA films can also be useful in the study of other distance-dependent interactions, such as electron transfer or distance-dependent quenching.

Original languageEnglish
Title of host publicationProceedings of SPIE - The International Society for Optical Engineering
PublisherPubl by Int Soc for Optical Engineering
Pages622-631
Number of pages10
Volume1640
ISBN (Print)0819407860
StatePublished - 1 Jan 1992
EventTime-Resolved Laser Spectroscopy in Biochemistry III - Los Angeles, CA, USA
Duration: 20 Jan 199222 Jan 1992

Other

OtherTime-Resolved Laser Spectroscopy in Biochemistry III
CityLos Angeles, CA, USA
Period20/01/9222/01/92

Fingerprint

Stretching
Full width at half maximum
Energy transfer
Conformations
Quenching
Alcohols
Electrons
tryptophan
electron transfer
alcohols
energy transfer
quenching
decay
interactions

Cite this

Gryczynski, I., Gryczynski, Z., Wiczk, W. M., Kusba, J., & Lakowicz, J. R. (1992). Effect of molecular ordering on distance distributions of flexible donor-acceptor pairs. In Proceedings of SPIE - The International Society for Optical Engineering (Vol. 1640, pp. 622-631). Publ by Int Soc for Optical Engineering.
Gryczynski, Ignacy ; Gryczynski, Zygmunt ; Wiczk, Wieslaw M. ; Kusba, Jozef ; Lakowicz, Joseph R. / Effect of molecular ordering on distance distributions of flexible donor-acceptor pairs. Proceedings of SPIE - The International Society for Optical Engineering. Vol. 1640 Publ by Int Soc for Optical Engineering, 1992. pp. 622-631
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Gryczynski, I, Gryczynski, Z, Wiczk, WM, Kusba, J & Lakowicz, JR 1992, Effect of molecular ordering on distance distributions of flexible donor-acceptor pairs. in Proceedings of SPIE - The International Society for Optical Engineering. vol. 1640, Publ by Int Soc for Optical Engineering, pp. 622-631, Time-Resolved Laser Spectroscopy in Biochemistry III, Los Angeles, CA, USA, 20/01/92.

Effect of molecular ordering on distance distributions of flexible donor-acceptor pairs. / Gryczynski, Ignacy; Gryczynski, Zygmunt; Wiczk, Wieslaw M.; Kusba, Jozef; Lakowicz, Joseph R.

Proceedings of SPIE - The International Society for Optical Engineering. Vol. 1640 Publ by Int Soc for Optical Engineering, 1992. p. 622-631.

Research output: Chapter in Book/Report/Conference proceedingConference contributionResearchpeer-review

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Gryczynski I, Gryczynski Z, Wiczk WM, Kusba J, Lakowicz JR. Effect of molecular ordering on distance distributions of flexible donor-acceptor pairs. In Proceedings of SPIE - The International Society for Optical Engineering. Vol. 1640. Publ by Int Soc for Optical Engineering. 1992. p. 622-631