Effect of molecular ordering on distance distributions of flexible donor-acceptor pairs

Ignacy Gryczynski, Zygmunt Gryczynski, Wieslaw M. Wiczk, Jozef Kusba, Joseph R. Lakowicz

Research output: Chapter in Book/Report/Conference proceedingConference contribution

7 Scopus citations

Abstract

Measurements of time-resolved intramolecular energy transfer in progressively stretched poly(vinyl alcohol) films were performed. The donor (tryptophan) and acceptor (dansyl) were linked with flexible polymethylene chain. Distance distributions were recovered from frequency-domain measurements of the donor decay. In isotropic PVA (in solution) a wide range of distances were detected (Gaussian full width at half maximum of about 16 angstroms) with an average distance of 13 angstroms. The donor-acceptor distance distribution became progressively more narrow when the PVA films were stretched. Four-fold or more stretching results in a single donor-acceptor distance. The maximal measured donor-acceptor distance of 23 angstroms is in excellent agreement with the computed distance for the fully stretched conformation. The possibility of partial and/or full ordering of polymethylene chains by stretching the PVA films can also be useful in the study of other distance-dependent interactions, such as electron transfer or distance-dependent quenching.

Original languageEnglish
Title of host publicationProceedings of SPIE - The International Society for Optical Engineering
PublisherPubl by Int Soc for Optical Engineering
Pages622-631
Number of pages10
Volume1640
ISBN (Print)0819407860
StatePublished - 1 Jan 1992
EventTime-Resolved Laser Spectroscopy in Biochemistry III - Los Angeles, CA, USA
Duration: 20 Jan 199222 Jan 1992

Other

OtherTime-Resolved Laser Spectroscopy in Biochemistry III
CityLos Angeles, CA, USA
Period20/01/9222/01/92

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