Abstract
Measurements of time-resolved intramolecular energy transfer in progressively stretched poly(vinyl alcohol) films were performed. The donor (tryptophan) and acceptor (dansyl) were linked with flexible polymethylene chain. Distance distributions were recovered from frequency-domain measurements of the donor decay. In isotropic PVA (in solution) a wide range of distances were detected (Gaussian full width at half maximum of about 16 angstroms) with an average distance of 13 angstroms. The donor-acceptor distance distribution became progressively more narrow when the PVA films were stretched. Four-fold or more stretching results in a single donor-acceptor distance. The maximal measured donor-acceptor distance of 23 angstroms is in excellent agreement with the computed distance for the fully stretched conformation. The possibility of partial and/or full ordering of polymethylene chains by stretching the PVA films can also be useful in the study of other distance-dependent interactions, such as electron transfer or distance-dependent quenching.
| Original language | English |
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| Title of host publication | Proceedings of SPIE - The International Society for Optical Engineering |
| Publisher | Publ by Int Soc for Optical Engineering |
| Pages | 622-631 |
| Number of pages | 10 |
| Volume | 1640 |
| ISBN (Print) | 0819407860 |
| State | Published - 1 Jan 1992 |
| Event | Time-Resolved Laser Spectroscopy in Biochemistry III - Los Angeles, CA, USA Duration: 20 Jan 1992 → 22 Jan 1992 |
Other
| Other | Time-Resolved Laser Spectroscopy in Biochemistry III |
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| City | Los Angeles, CA, USA |
| Period | 20/01/92 → 22/01/92 |