TY - JOUR
T1 - Effect of methamidophos on soil fungi community in microcosms by plate count, DGGE and clone library analysis
AU - LI, Xinyu
AU - ZHANG, Huiwen
AU - WU, Minna
AU - ZHANG, Yan
AU - ZHANG, Chenggang
N1 - Funding Information:
This work was supported by the National Basic Research Program (973) of China (No. 2004CB418503) and the Doctoral Program of Institute of Applied Ecology, Chinese Academy of Sciences (No. C17DC06SBS31001).
PY - 2008
Y1 - 2008
N2 - Methamidophos was widely used a pesticide in northern China. The potential influences of methamidophos on soil fungal community in black soil were assessed by plate count, 28S rDNA-PCR-DGGE, and clone library analysis. Three methamidophos levels (50, 150, and 250 mg/kg) were tested in soil microcosms. Results from plate count during a 60-d microcosm experiment showed that high concentrations of methamidophos (250 mg/kg) could significantly stimulate fungal populations. DGGE (denaturing gradient gel electrophoresis) fingerprinting patterns showed a significant difference between the responses of culturable and total fungi communities under the stress of methamidophos. Shannon diversity indices calculated from DGGE profiles indicated that culturable fungi in all microcosms with methamidophos treatment increased after 1 week of incubation. However, the diversity indices of total fungi decreased in the first week, as compared to the stimulation of culturable fungi. At the 8th week, however, all the microcosms treated by methamidophos were similar to the control microcosms in community structure as suggested by the Shannon diversity indices for both culturable and total fungi. In contrast, after 1 week the fungal structure of culturable and unculturable both were disturbed to different extent under the stresses of methamidophos by clustering analysis. Clone sequencing analysis indicated the stimulation of pathogenic and unculturable fungal populations by methamidophos treatment, suggetsing potential risks of plant disease outbreak.
AB - Methamidophos was widely used a pesticide in northern China. The potential influences of methamidophos on soil fungal community in black soil were assessed by plate count, 28S rDNA-PCR-DGGE, and clone library analysis. Three methamidophos levels (50, 150, and 250 mg/kg) were tested in soil microcosms. Results from plate count during a 60-d microcosm experiment showed that high concentrations of methamidophos (250 mg/kg) could significantly stimulate fungal populations. DGGE (denaturing gradient gel electrophoresis) fingerprinting patterns showed a significant difference between the responses of culturable and total fungi communities under the stress of methamidophos. Shannon diversity indices calculated from DGGE profiles indicated that culturable fungi in all microcosms with methamidophos treatment increased after 1 week of incubation. However, the diversity indices of total fungi decreased in the first week, as compared to the stimulation of culturable fungi. At the 8th week, however, all the microcosms treated by methamidophos were similar to the control microcosms in community structure as suggested by the Shannon diversity indices for both culturable and total fungi. In contrast, after 1 week the fungal structure of culturable and unculturable both were disturbed to different extent under the stresses of methamidophos by clustering analysis. Clone sequencing analysis indicated the stimulation of pathogenic and unculturable fungal populations by methamidophos treatment, suggetsing potential risks of plant disease outbreak.
KW - DGGE (denaturing gradient gel electrophoresis)
KW - clone sequencing
KW - fungi diversity
KW - methamidophos
UR - http://www.scopus.com/inward/record.url?scp=43449089492&partnerID=8YFLogxK
U2 - 10.1016/S1001-0742(08)62103-8
DO - 10.1016/S1001-0742(08)62103-8
M3 - Article
C2 - 18575117
AN - SCOPUS:43449089492
SN - 1001-0742
VL - 20
SP - 619
EP - 625
JO - Journal of Environmental Sciences
JF - Journal of Environmental Sciences
IS - 5
ER -