Effect of methamidophos on soil fungi community in microcosms by plate count, DGGE and clone library analysis

Xinyu LI, Huiwen ZHANG, Minna WU, Yan Zhang, Chenggang ZHANG

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14 Citations (Scopus)

Abstract

Methamidophos was widely used a pesticide in northern China. The potential influences of methamidophos on soil fungal community in black soil were assessed by plate count, 28S rDNA-PCR-DGGE, and clone library analysis. Three methamidophos levels (50, 150, and 250 mg/kg) were tested in soil microcosms. Results from plate count during a 60-d microcosm experiment showed that high concentrations of methamidophos (250 mg/kg) could significantly stimulate fungal populations. DGGE (denaturing gradient gel electrophoresis) fingerprinting patterns showed a significant difference between the responses of culturable and total fungi communities under the stress of methamidophos. Shannon diversity indices calculated from DGGE profiles indicated that culturable fungi in all microcosms with methamidophos treatment increased after 1 week of incubation. However, the diversity indices of total fungi decreased in the first week, as compared to the stimulation of culturable fungi. At the 8th week, however, all the microcosms treated by methamidophos were similar to the control microcosms in community structure as suggested by the Shannon diversity indices for both culturable and total fungi. In contrast, after 1 week the fungal structure of culturable and unculturable both were disturbed to different extent under the stresses of methamidophos by clustering analysis. Clone sequencing analysis indicated the stimulation of pathogenic and unculturable fungal populations by methamidophos treatment, suggetsing potential risks of plant disease outbreak.

Original languageEnglish
Pages (from-to)619-625
Number of pages7
JournalJournal of Environmental Sciences
Volume20
Issue number5
DOIs
StatePublished - 19 May 2008

Fingerprint

Electrophoresis
Fungi
microcosm
clone
electrokinesis
Gels
gel
fungus
Soils
diversity index
soil
Pesticides
community structure
pesticide
incubation
analysis
effect
library
methamidophos
Ribosomal DNA

Keywords

  • clone sequencing
  • DGGE (denaturing gradient gel electrophoresis)
  • fungi diversity
  • methamidophos

Cite this

@article{e76910f6c304452695fff29a4da1d1b7,
title = "Effect of methamidophos on soil fungi community in microcosms by plate count, DGGE and clone library analysis",
abstract = "Methamidophos was widely used a pesticide in northern China. The potential influences of methamidophos on soil fungal community in black soil were assessed by plate count, 28S rDNA-PCR-DGGE, and clone library analysis. Three methamidophos levels (50, 150, and 250 mg/kg) were tested in soil microcosms. Results from plate count during a 60-d microcosm experiment showed that high concentrations of methamidophos (250 mg/kg) could significantly stimulate fungal populations. DGGE (denaturing gradient gel electrophoresis) fingerprinting patterns showed a significant difference between the responses of culturable and total fungi communities under the stress of methamidophos. Shannon diversity indices calculated from DGGE profiles indicated that culturable fungi in all microcosms with methamidophos treatment increased after 1 week of incubation. However, the diversity indices of total fungi decreased in the first week, as compared to the stimulation of culturable fungi. At the 8th week, however, all the microcosms treated by methamidophos were similar to the control microcosms in community structure as suggested by the Shannon diversity indices for both culturable and total fungi. In contrast, after 1 week the fungal structure of culturable and unculturable both were disturbed to different extent under the stresses of methamidophos by clustering analysis. Clone sequencing analysis indicated the stimulation of pathogenic and unculturable fungal populations by methamidophos treatment, suggetsing potential risks of plant disease outbreak.",
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Effect of methamidophos on soil fungi community in microcosms by plate count, DGGE and clone library analysis. / LI, Xinyu; ZHANG, Huiwen; WU, Minna; Zhang, Yan; ZHANG, Chenggang.

In: Journal of Environmental Sciences, Vol. 20, No. 5, 19.05.2008, p. 619-625.

Research output: Contribution to journalArticleResearchpeer-review

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T1 - Effect of methamidophos on soil fungi community in microcosms by plate count, DGGE and clone library analysis

AU - LI, Xinyu

AU - ZHANG, Huiwen

AU - WU, Minna

AU - Zhang, Yan

AU - ZHANG, Chenggang

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