Effect of human neural precursor cell transplantation on endogenous neurogenesis after focal cerebral ischemia in the rat

Kunlin Jin, Lin Xie, Xiaoou Mao, Maeve B. Greenberg, Alexander Moore, Botao Peng, Rose B. Greenberg, David A. Greenberg

Research output: Contribution to journalArticle

64 Citations (Scopus)

Abstract

Little is known about the relationship between neuronal cell transplantation and endogenous neurogenesis after experimental stroke. We found previously that transplantation of neuronal precursors derived from BG01 human embryonic stem cells reduced infarct volume and improved behavioral outcome after distal middle cerebral artery occlusion (MCAO) in rats. In this study, transplantation was performed 14 days after distal MCAO and doublecortin (Dcx)-expressing cells in the subventricular zone (SVZ) and subgranular zone of dentate gyrus (SGZ) were counted 60 days post-transplant. Transplantation increased neurogenesis (Dcx expression) in ipsilateral SVZ, but not in contralateral SVZ or either SGZ, in both young adult (3-month-old) and aged (24-month-old) rats. These findings suggest that cell-based therapy for stroke may be associated with changes in endogenous adaptive processes, including neurogenesis.

Original languageEnglish
Pages (from-to)56-62
Number of pages7
JournalBrain Research
Volume1374
DOIs
StatePublished - 16 Feb 2011

Fingerprint

Lateral Ventricles
Cell Transplantation
Neurogenesis
Brain Ischemia
Transplantation
Middle Cerebral Artery Infarction
Stroke
Dentate Gyrus
Cell- and Tissue-Based Therapy
Young Adult
Transplants

Keywords

  • BrdU
  • Bromodeoxyuridine
  • DG
  • Dcx
  • GFAP
  • HuN
  • IC
  • ICV
  • IV
  • MCAO
  • MSC
  • NPC
  • NeuN
  • SGZ
  • SVZ
  • dentate gyrus
  • doublecortin
  • glial fibrillary acidic protein
  • hESC
  • human embryonic stem cell
  • human nuclear antigen
  • intracerebral
  • intracerebroventricular
  • intravenous
  • mesenchymal stem cell
  • middle cerebral artery occlusion
  • neuronal nuclear antigen
  • neuronal precursor cells
  • subgranular zone
  • subventricular zone

Cite this

Jin, Kunlin ; Xie, Lin ; Mao, Xiaoou ; Greenberg, Maeve B. ; Moore, Alexander ; Peng, Botao ; Greenberg, Rose B. ; Greenberg, David A. / Effect of human neural precursor cell transplantation on endogenous neurogenesis after focal cerebral ischemia in the rat. In: Brain Research. 2011 ; Vol. 1374. pp. 56-62.
@article{60db85b37b9840bf945e5d33acddd939,
title = "Effect of human neural precursor cell transplantation on endogenous neurogenesis after focal cerebral ischemia in the rat",
abstract = "Little is known about the relationship between neuronal cell transplantation and endogenous neurogenesis after experimental stroke. We found previously that transplantation of neuronal precursors derived from BG01 human embryonic stem cells reduced infarct volume and improved behavioral outcome after distal middle cerebral artery occlusion (MCAO) in rats. In this study, transplantation was performed 14 days after distal MCAO and doublecortin (Dcx)-expressing cells in the subventricular zone (SVZ) and subgranular zone of dentate gyrus (SGZ) were counted 60 days post-transplant. Transplantation increased neurogenesis (Dcx expression) in ipsilateral SVZ, but not in contralateral SVZ or either SGZ, in both young adult (3-month-old) and aged (24-month-old) rats. These findings suggest that cell-based therapy for stroke may be associated with changes in endogenous adaptive processes, including neurogenesis.",
keywords = "BrdU, Bromodeoxyuridine, DG, Dcx, GFAP, HuN, IC, ICV, IV, MCAO, MSC, NPC, NeuN, SGZ, SVZ, dentate gyrus, doublecortin, glial fibrillary acidic protein, hESC, human embryonic stem cell, human nuclear antigen, intracerebral, intracerebroventricular, intravenous, mesenchymal stem cell, middle cerebral artery occlusion, neuronal nuclear antigen, neuronal precursor cells, subgranular zone, subventricular zone",
author = "Kunlin Jin and Lin Xie and Xiaoou Mao and Greenberg, {Maeve B.} and Alexander Moore and Botao Peng and Greenberg, {Rose B.} and Greenberg, {David A.}",
year = "2011",
month = "2",
day = "16",
doi = "10.1016/j.brainres.2010.12.037",
language = "English",
volume = "1374",
pages = "56--62",
journal = "Brain Research",
issn = "0006-8993",
publisher = "Elsevier",

}

Effect of human neural precursor cell transplantation on endogenous neurogenesis after focal cerebral ischemia in the rat. / Jin, Kunlin; Xie, Lin; Mao, Xiaoou; Greenberg, Maeve B.; Moore, Alexander; Peng, Botao; Greenberg, Rose B.; Greenberg, David A.

In: Brain Research, Vol. 1374, 16.02.2011, p. 56-62.

Research output: Contribution to journalArticle

TY - JOUR

T1 - Effect of human neural precursor cell transplantation on endogenous neurogenesis after focal cerebral ischemia in the rat

AU - Jin, Kunlin

AU - Xie, Lin

AU - Mao, Xiaoou

AU - Greenberg, Maeve B.

AU - Moore, Alexander

AU - Peng, Botao

AU - Greenberg, Rose B.

AU - Greenberg, David A.

PY - 2011/2/16

Y1 - 2011/2/16

N2 - Little is known about the relationship between neuronal cell transplantation and endogenous neurogenesis after experimental stroke. We found previously that transplantation of neuronal precursors derived from BG01 human embryonic stem cells reduced infarct volume and improved behavioral outcome after distal middle cerebral artery occlusion (MCAO) in rats. In this study, transplantation was performed 14 days after distal MCAO and doublecortin (Dcx)-expressing cells in the subventricular zone (SVZ) and subgranular zone of dentate gyrus (SGZ) were counted 60 days post-transplant. Transplantation increased neurogenesis (Dcx expression) in ipsilateral SVZ, but not in contralateral SVZ or either SGZ, in both young adult (3-month-old) and aged (24-month-old) rats. These findings suggest that cell-based therapy for stroke may be associated with changes in endogenous adaptive processes, including neurogenesis.

AB - Little is known about the relationship between neuronal cell transplantation and endogenous neurogenesis after experimental stroke. We found previously that transplantation of neuronal precursors derived from BG01 human embryonic stem cells reduced infarct volume and improved behavioral outcome after distal middle cerebral artery occlusion (MCAO) in rats. In this study, transplantation was performed 14 days after distal MCAO and doublecortin (Dcx)-expressing cells in the subventricular zone (SVZ) and subgranular zone of dentate gyrus (SGZ) were counted 60 days post-transplant. Transplantation increased neurogenesis (Dcx expression) in ipsilateral SVZ, but not in contralateral SVZ or either SGZ, in both young adult (3-month-old) and aged (24-month-old) rats. These findings suggest that cell-based therapy for stroke may be associated with changes in endogenous adaptive processes, including neurogenesis.

KW - BrdU

KW - Bromodeoxyuridine

KW - DG

KW - Dcx

KW - GFAP

KW - HuN

KW - IC

KW - ICV

KW - IV

KW - MCAO

KW - MSC

KW - NPC

KW - NeuN

KW - SGZ

KW - SVZ

KW - dentate gyrus

KW - doublecortin

KW - glial fibrillary acidic protein

KW - hESC

KW - human embryonic stem cell

KW - human nuclear antigen

KW - intracerebral

KW - intracerebroventricular

KW - intravenous

KW - mesenchymal stem cell

KW - middle cerebral artery occlusion

KW - neuronal nuclear antigen

KW - neuronal precursor cells

KW - subgranular zone

KW - subventricular zone

UR - http://www.scopus.com/inward/record.url?scp=79251635274&partnerID=8YFLogxK

U2 - 10.1016/j.brainres.2010.12.037

DO - 10.1016/j.brainres.2010.12.037

M3 - Article

C2 - 21167824

AN - SCOPUS:79251635274

VL - 1374

SP - 56

EP - 62

JO - Brain Research

JF - Brain Research

SN - 0006-8993

ER -