Effect of human neural precursor cell transplantation on endogenous neurogenesis after focal cerebral ischemia in the rat

Kunlin Jin, Lin Xie, Xiaoou Mao, Maeve B. Greenberg, Alexander Moore, Botao Peng, Rose B. Greenberg, David A. Greenberg

Research output: Contribution to journalArticle

69 Scopus citations

Abstract

Little is known about the relationship between neuronal cell transplantation and endogenous neurogenesis after experimental stroke. We found previously that transplantation of neuronal precursors derived from BG01 human embryonic stem cells reduced infarct volume and improved behavioral outcome after distal middle cerebral artery occlusion (MCAO) in rats. In this study, transplantation was performed 14 days after distal MCAO and doublecortin (Dcx)-expressing cells in the subventricular zone (SVZ) and subgranular zone of dentate gyrus (SGZ) were counted 60 days post-transplant. Transplantation increased neurogenesis (Dcx expression) in ipsilateral SVZ, but not in contralateral SVZ or either SGZ, in both young adult (3-month-old) and aged (24-month-old) rats. These findings suggest that cell-based therapy for stroke may be associated with changes in endogenous adaptive processes, including neurogenesis.

Original languageEnglish
Pages (from-to)56-62
Number of pages7
JournalBrain Research
Volume1374
DOIs
StatePublished - 16 Feb 2011

Keywords

  • BrdU
  • Bromodeoxyuridine
  • DG
  • Dcx
  • GFAP
  • HuN
  • IC
  • ICV
  • IV
  • MCAO
  • MSC
  • NPC
  • NeuN
  • SGZ
  • SVZ
  • dentate gyrus
  • doublecortin
  • glial fibrillary acidic protein
  • hESC
  • human embryonic stem cell
  • human nuclear antigen
  • intracerebral
  • intracerebroventricular
  • intravenous
  • mesenchymal stem cell
  • middle cerebral artery occlusion
  • neuronal nuclear antigen
  • neuronal precursor cells
  • subgranular zone
  • subventricular zone

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