TY - JOUR
T1 - Effect of furosemide on unidirectional fluxes of sodium and chloride across the skin of the frog, Rana pipiens
AU - Yorio, Thomas
AU - Bentley, P. J.
N1 - Funding Information:
This work was supported by National Science Foundation Grant No. BMS 75-07684.
PY - 1976/12/14
Y1 - 1976/12/14
N2 - 1. 1. The diuretic furosemide, when added to the outside solution at a concentration of 5 · 10-4 M, increases the electrical potential difference (PD) across the isolated frog skin, but the short-circuit (Isc) is unchanged. Lower concentrations had no significant effect on these electrical parameters. 2. 2. When SO4 2- or NO3 - are substituted for Cl- in the Ringer's solution furosemide has no effect on the PD or Isc. 3. 3. Simultaneous unidirectional fluxes of Na+ and Cl- show that furosemide (5 · 10-4 M outside) reduces both the influx and outflux of Cl-, while the Na+ fluxes are not altered. 4. 4. Furosemide (5 · 10-4 M) on the corium side of the frog skin had no significant effect on either PD, Isc or unidirectional fluxes of Cl-. 5. 5. It is suggested that furosemide reduces passive Cl- transfer, possibly by interacting with the Cl-/Cl- exchange diffusion mechanism which has been observed in this tissue. These observations further suggest that perhaps the diuretic action of furosemide may be mediated by such an effect on passive Cl- permeability which is linked to the active Cl- transport mechanism in the renal tubule.
AB - 1. 1. The diuretic furosemide, when added to the outside solution at a concentration of 5 · 10-4 M, increases the electrical potential difference (PD) across the isolated frog skin, but the short-circuit (Isc) is unchanged. Lower concentrations had no significant effect on these electrical parameters. 2. 2. When SO4 2- or NO3 - are substituted for Cl- in the Ringer's solution furosemide has no effect on the PD or Isc. 3. 3. Simultaneous unidirectional fluxes of Na+ and Cl- show that furosemide (5 · 10-4 M outside) reduces both the influx and outflux of Cl-, while the Na+ fluxes are not altered. 4. 4. Furosemide (5 · 10-4 M) on the corium side of the frog skin had no significant effect on either PD, Isc or unidirectional fluxes of Cl-. 5. 5. It is suggested that furosemide reduces passive Cl- transfer, possibly by interacting with the Cl-/Cl- exchange diffusion mechanism which has been observed in this tissue. These observations further suggest that perhaps the diuretic action of furosemide may be mediated by such an effect on passive Cl- permeability which is linked to the active Cl- transport mechanism in the renal tubule.
UR - http://www.scopus.com/inward/record.url?scp=0017107460&partnerID=8YFLogxK
U2 - 10.1016/0005-2736(76)90053-5
DO - 10.1016/0005-2736(76)90053-5
M3 - Article
C2 - 1087163
AN - SCOPUS:0017107460
SN - 0005-2736
VL - 455
SP - 831
EP - 836
JO - BBA - Biomembranes
JF - BBA - Biomembranes
IS - 3
ER -