Effect of age and caloric restriction on DNA oxidative damage in different tissues of C57BL/6 mice

R. S. Sohal, Sanjiv Agarwal, Mehmet Candas, Michael J. Forster, Harbans Lal

Research output: Contribution to journalArticle

299 Scopus citations

Abstract

The objective of this study was to explore the role of molecular oxidative damage and caloric intake in the aging process. The concentration of 8-hydroxydeoxyguanosine (8-OHdG), a product of DNA oxidation, was compared in five different tissues of mice (skeletal muscle, brain, heart, liver and kidney) as a function of age and in response to dietary restriction. A comparison of 8- and 27-month-old mice indicated that the age-related increase in 8-OHdG concentration was greater in skeletal muscle, brain and heart, which are primarily composed of long-lived, post-mitotic cells, than in liver and kidney, which consist of slow-dividing cells. Dietary restricted (DR) mice kept on 60% caloric intake as compared to the ad libitum-fed (AL) mice showed a lower concentration in 8-OHdG content in all the tissues compared to AL mice. The DR-related amelioration of DNA oxidative damage was greater in the post-mitotic tissues compared to those undergoing slow mitoses. Results support the hypothesis that oxidative damage to long-lived post-mitotic cells may be a key factor in the aging process.

Original languageEnglish
Pages (from-to)215-224
Number of pages10
JournalMechanisms of Ageing and Development
Volume76
Issue number2-3
DOIs
StatePublished - 20 Oct 1994

Keywords

  • Aging
  • DNA damage
  • Dietary restriction
  • Free radicals
  • Oxidative stress

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