Effect of age and caloric restriction on DNA oxidative damage in different tissues of C57BL/6 mice

R. S. Sohal, Sanjiv Agarwal, Mehmet Candas, Michael J. Forster, Harbans Lal

Research output: Contribution to journalArticlepeer-review

312 Scopus citations

Abstract

The objective of this study was to explore the role of molecular oxidative damage and caloric intake in the aging process. The concentration of 8-hydroxydeoxyguanosine (8-OHdG), a product of DNA oxidation, was compared in five different tissues of mice (skeletal muscle, brain, heart, liver and kidney) as a function of age and in response to dietary restriction. A comparison of 8- and 27-month-old mice indicated that the age-related increase in 8-OHdG concentration was greater in skeletal muscle, brain and heart, which are primarily composed of long-lived, post-mitotic cells, than in liver and kidney, which consist of slow-dividing cells. Dietary restricted (DR) mice kept on 60% caloric intake as compared to the ad libitum-fed (AL) mice showed a lower concentration in 8-OHdG content in all the tissues compared to AL mice. The DR-related amelioration of DNA oxidative damage was greater in the post-mitotic tissues compared to those undergoing slow mitoses. Results support the hypothesis that oxidative damage to long-lived post-mitotic cells may be a key factor in the aging process.

Original languageEnglish
Pages (from-to)215-224
Number of pages10
JournalMechanisms of Ageing and Development
Volume76
Issue number2-3
DOIs
StatePublished - 20 Oct 1994

Keywords

  • Aging
  • DNA damage
  • Dietary restriction
  • Free radicals
  • Oxidative stress

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