Effect of age and caloric restriction on DNA oxidative damage in different tissues of C57BL/6 mice

R. S. Sohal; Sanjiv Agarwal; Mehmet Candas; Michael J. Forster; Harbans Lal

doi:10.1016/0047-6374(94)91595-4

Effect of age and caloric restriction on DNA oxidative damage in different tissues of C57BL/6 mice

R. S. Sohal, Sanjiv Agarwal, Mehmet Candas, Michael J. Forster, Harbans Lal

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Abstract

The objective of this study was to explore the role of molecular oxidative damage and caloric intake in the aging process. The concentration of 8-hydroxydeoxyguanosine (8-OHdG), a product of DNA oxidation, was compared in five different tissues of mice (skeletal muscle, brain, heart, liver and kidney) as a function of age and in response to dietary restriction. A comparison of 8- and 27-month-old mice indicated that the age-related increase in 8-OHdG concentration was greater in skeletal muscle, brain and heart, which are primarily composed of long-lived, post-mitotic cells, than in liver and kidney, which consist of slow-dividing cells. Dietary restricted (DR) mice kept on 60% caloric intake as compared to the ad libitum-fed (AL) mice showed a lower concentration in 8-OHdG content in all the tissues compared to AL mice. The DR-related amelioration of DNA oxidative damage was greater in the post-mitotic tissues compared to those undergoing slow mitoses. Results support the hypothesis that oxidative damage to long-lived post-mitotic cells may be a key factor in the aging process.

Original language	English
Pages (from-to)	215-224
Number of pages	10
Journal	Mechanisms of Ageing and Development
Volume	76
Issue number	2-3
DOIs	https://doi.org/10.1016/0047-6374(94)91595-4
State	Published - 20 Oct 1994

Keywords

Aging
DNA damage
Dietary restriction
Free radicals
Oxidative stress

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10.1016/0047-6374(94)91595-4

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title = "Effect of age and caloric restriction on DNA oxidative damage in different tissues of C57BL/6 mice",

abstract = "The objective of this study was to explore the role of molecular oxidative damage and caloric intake in the aging process. The concentration of 8-hydroxydeoxyguanosine (8-OHdG), a product of DNA oxidation, was compared in five different tissues of mice (skeletal muscle, brain, heart, liver and kidney) as a function of age and in response to dietary restriction. A comparison of 8- and 27-month-old mice indicated that the age-related increase in 8-OHdG concentration was greater in skeletal muscle, brain and heart, which are primarily composed of long-lived, post-mitotic cells, than in liver and kidney, which consist of slow-dividing cells. Dietary restricted (DR) mice kept on 60% caloric intake as compared to the ad libitum-fed (AL) mice showed a lower concentration in 8-OHdG content in all the tissues compared to AL mice. The DR-related amelioration of DNA oxidative damage was greater in the post-mitotic tissues compared to those undergoing slow mitoses. Results support the hypothesis that oxidative damage to long-lived post-mitotic cells may be a key factor in the aging process.",

keywords = "Aging, DNA damage, Dietary restriction, Free radicals, Oxidative stress",

author = "Sohal, {R. S.} and Sanjiv Agarwal and Mehmet Candas and Forster, {Michael J.} and Harbans Lal",

note = "Funding Information: This study was supported by the grants R01AG7695 and R01AG7657 from the National Institutes of Health --National Institute on Aging. The technical assistance of Miss Kim Dawson and Miss Kshama Jaiswal is gratefully acknowledged.",

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AU - Sohal, R. S.

AU - Agarwal, Sanjiv

AU - Candas, Mehmet

AU - Forster, Michael J.

AU - Lal, Harbans

N1 - Funding Information: This study was supported by the grants R01AG7695 and R01AG7657 from the National Institutes of Health --National Institute on Aging. The technical assistance of Miss Kim Dawson and Miss Kshama Jaiswal is gratefully acknowledged.

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Y1 - 1994/10/20

N2 - The objective of this study was to explore the role of molecular oxidative damage and caloric intake in the aging process. The concentration of 8-hydroxydeoxyguanosine (8-OHdG), a product of DNA oxidation, was compared in five different tissues of mice (skeletal muscle, brain, heart, liver and kidney) as a function of age and in response to dietary restriction. A comparison of 8- and 27-month-old mice indicated that the age-related increase in 8-OHdG concentration was greater in skeletal muscle, brain and heart, which are primarily composed of long-lived, post-mitotic cells, than in liver and kidney, which consist of slow-dividing cells. Dietary restricted (DR) mice kept on 60% caloric intake as compared to the ad libitum-fed (AL) mice showed a lower concentration in 8-OHdG content in all the tissues compared to AL mice. The DR-related amelioration of DNA oxidative damage was greater in the post-mitotic tissues compared to those undergoing slow mitoses. Results support the hypothesis that oxidative damage to long-lived post-mitotic cells may be a key factor in the aging process.

AB - The objective of this study was to explore the role of molecular oxidative damage and caloric intake in the aging process. The concentration of 8-hydroxydeoxyguanosine (8-OHdG), a product of DNA oxidation, was compared in five different tissues of mice (skeletal muscle, brain, heart, liver and kidney) as a function of age and in response to dietary restriction. A comparison of 8- and 27-month-old mice indicated that the age-related increase in 8-OHdG concentration was greater in skeletal muscle, brain and heart, which are primarily composed of long-lived, post-mitotic cells, than in liver and kidney, which consist of slow-dividing cells. Dietary restricted (DR) mice kept on 60% caloric intake as compared to the ad libitum-fed (AL) mice showed a lower concentration in 8-OHdG content in all the tissues compared to AL mice. The DR-related amelioration of DNA oxidative damage was greater in the post-mitotic tissues compared to those undergoing slow mitoses. Results support the hypothesis that oxidative damage to long-lived post-mitotic cells may be a key factor in the aging process.

KW - Aging

KW - DNA damage

KW - Dietary restriction

KW - Free radicals

KW - Oxidative stress

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Effect of age and caloric restriction on DNA oxidative damage in different tissues of C57BL/6 mice

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