Dramatic Increases in Resonance Energy Transfer Have Been Observed Between Fluorophores Bound to DNA Above Metallic Silver Islands: Opportunities for Long-Range Immunoassays and New DNA Arrays

Ignacy Gryczynski; Joanna Malicka; Zygmunt Gryczynski; Joseph R. Lakowicz; Chris D. Geddes

doi:10.1023/A:1016827826417

Dramatic Increases in Resonance Energy Transfer Have Been Observed Between Fluorophores Bound to DNA Above Metallic Silver Islands: Opportunities for Long-Range Immunoassays and New DNA Arrays

Ignacy Gryczynski, Joanna Malicka, Zygmunt Gryczynski, Joseph R. Lakowicz, Chris D. Geddes

Research output: Contribution to journal › Article › peer-review

12 Scopus citations

Abstract

The dramatic increase in resonance energy transfer (RET) between fluorophores bound to DNA above metallic silver islands is discussed. In nearly all cases of resonance energy transfer (RET), its usefulness as a spectroscopic ruler is determined by the magnitude of the Förster distance, R _o. For the most favorable case of high spectral overlap and high quantum yield, the maximum value of R_o is near 55 Å for organic fluorophores and up to 90 Å for lanthanide donors. It is found that these short free-space Förster distances may be substantially increased by placing the donor and acceptors pairs above silver island films.

Original language	English
Article number	375482
Pages (from-to)	131-133
Number of pages	3
Journal	Journal of Fluorescence
Volume	12
Issue number	2
DOIs	https://doi.org/10.1023/A:1016827826417
State	Published - 2002

Keywords

DNA arrays
Enhanced resonance energy transfer (RET)
Immunoassays

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10.1023/A:1016827826417

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Gryczynski, I., Malicka, J., Gryczynski, Z., Lakowicz, J. R., & Geddes, C. D. (2002). Dramatic Increases in Resonance Energy Transfer Have Been Observed Between Fluorophores Bound to DNA Above Metallic Silver Islands: Opportunities for Long-Range Immunoassays and New DNA Arrays. Journal of Fluorescence, 12(2), 131-133. [375482]. https://doi.org/10.1023/A:1016827826417

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title = "Dramatic Increases in Resonance Energy Transfer Have Been Observed Between Fluorophores Bound to DNA Above Metallic Silver Islands: Opportunities for Long-Range Immunoassays and New DNA Arrays",

abstract = "The dramatic increase in resonance energy transfer (RET) between fluorophores bound to DNA above metallic silver islands is discussed. In nearly all cases of resonance energy transfer (RET), its usefulness as a spectroscopic ruler is determined by the magnitude of the F{\"o}rster distance, R o. For the most favorable case of high spectral overlap and high quantum yield, the maximum value of Ro is near 55 {\AA} for organic fluorophores and up to 90 {\AA} for lanthanide donors. It is found that these short free-space F{\"o}rster distances may be substantially increased by placing the donor and acceptors pairs above silver island films.",

keywords = "DNA arrays, Enhanced resonance energy transfer (RET), Immunoassays",

author = "Ignacy Gryczynski and Joanna Malicka and Zygmunt Gryczynski and Lakowicz, {Joseph R.} and Geddes, {Chris D.}",

year = "2002",

doi = "10.1023/A:1016827826417",

language = "English",

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pages = "131--133",

journal = "Journal of Fluorescence",

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Gryczynski, I, Malicka, J, Gryczynski, Z, Lakowicz, JR & Geddes, CD 2002, 'Dramatic Increases in Resonance Energy Transfer Have Been Observed Between Fluorophores Bound to DNA Above Metallic Silver Islands: Opportunities for Long-Range Immunoassays and New DNA Arrays', Journal of Fluorescence, vol. 12, no. 2, 375482, pp. 131-133. https://doi.org/10.1023/A:1016827826417

Dramatic Increases in Resonance Energy Transfer Have Been Observed Between Fluorophores Bound to DNA Above Metallic Silver Islands : Opportunities for Long-Range Immunoassays and New DNA Arrays. / Gryczynski, Ignacy; Malicka, Joanna; Gryczynski, Zygmunt et al.

In: Journal of Fluorescence, Vol. 12, No. 2, 375482, 2002, p. 131-133.

Research output: Contribution to journal › Article › peer-review

TY - JOUR

T1 - Dramatic Increases in Resonance Energy Transfer Have Been Observed Between Fluorophores Bound to DNA Above Metallic Silver Islands

T2 - Opportunities for Long-Range Immunoassays and New DNA Arrays

AU - Gryczynski, Ignacy

AU - Malicka, Joanna

AU - Gryczynski, Zygmunt

AU - Lakowicz, Joseph R.

AU - Geddes, Chris D.

PY - 2002

Y1 - 2002

N2 - The dramatic increase in resonance energy transfer (RET) between fluorophores bound to DNA above metallic silver islands is discussed. In nearly all cases of resonance energy transfer (RET), its usefulness as a spectroscopic ruler is determined by the magnitude of the Förster distance, R o. For the most favorable case of high spectral overlap and high quantum yield, the maximum value of Ro is near 55 Å for organic fluorophores and up to 90 Å for lanthanide donors. It is found that these short free-space Förster distances may be substantially increased by placing the donor and acceptors pairs above silver island films.

AB - The dramatic increase in resonance energy transfer (RET) between fluorophores bound to DNA above metallic silver islands is discussed. In nearly all cases of resonance energy transfer (RET), its usefulness as a spectroscopic ruler is determined by the magnitude of the Förster distance, R o. For the most favorable case of high spectral overlap and high quantum yield, the maximum value of Ro is near 55 Å for organic fluorophores and up to 90 Å for lanthanide donors. It is found that these short free-space Förster distances may be substantially increased by placing the donor and acceptors pairs above silver island films.

KW - DNA arrays

KW - Enhanced resonance energy transfer (RET)

KW - Immunoassays

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M3 - Article

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SN - 1053-0509

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SP - 131

EP - 133

JO - Journal of Fluorescence

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ER -

Dramatic Increases in Resonance Energy Transfer Have Been Observed Between Fluorophores Bound to DNA Above Metallic Silver Islands: Opportunities for Long-Range Immunoassays and New DNA Arrays

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