DNA hybridization using surface plasmon-coupled emission

Joanna Malicka, Ignacy Gryczynski, Zygmunt Gryczynski, Joseph R. Lakowicz

Research output: Contribution to journalConference article

Abstract

We described a new approach to measuring DNA hybridization using surface plasmon-coupled emission (SPCE). This phenomenon occurs for fluorophores within few hundreds of nanometers of a thin metal film on a glass substrate, in our case a 50 nm thick silver film. Excited fluorophores coupled with the surface plasmons in the metal resulting in directional emission through the glass substrate. We studied the emission of Cy3-labeled DNA oligomers bound to complementary unlabeled biotinylated-oligomers, which were bound to the metal surface via a streptavidin-BSA monolayer. Hybridization resulted in directional emission of Cy3-DNA into the prism. Additionally, the use of SPCE resulted in suppression of interfering emission from non-complementary Cy5-DNA oligomers due to weaker coupling of the more distant fluorophores with the surface plasmons. A large fraction of the total potential emission can couple to the surface plasmon resulting in improved sensitivity. We expect SPCE to have numerous applications to nucleic acid analyses.

Original languageEnglish
Pages (from-to)283-288
Number of pages6
JournalProceedings of SPIE - The International Society for Optical Engineering
Volume5321
DOIs
StatePublished - 27 Oct 2004
EventProgress in Biomedical Optics and Imaging - Biomedical Vibrational Spectroscopy and Biohazard Detection Technologies - San Jose, CA, United States
Duration: 25 Jan 200427 Jan 2004

Fingerprint

Surface Plasmon
DNA
deoxyribonucleic acid
Fluorophores
Oligomers
oligomers
Plasmons
Surface Plasmons
Metals
plasmons
Substrate
Glass
Streptavidin
Nucleic acids
Substrates
Prisms
glass
Silver
Weak Coupling
Prism

Keywords

  • Dna hybridization
  • Silver film
  • Surface plasmon-coupled emission

Cite this

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title = "DNA hybridization using surface plasmon-coupled emission",
abstract = "We described a new approach to measuring DNA hybridization using surface plasmon-coupled emission (SPCE). This phenomenon occurs for fluorophores within few hundreds of nanometers of a thin metal film on a glass substrate, in our case a 50 nm thick silver film. Excited fluorophores coupled with the surface plasmons in the metal resulting in directional emission through the glass substrate. We studied the emission of Cy3-labeled DNA oligomers bound to complementary unlabeled biotinylated-oligomers, which were bound to the metal surface via a streptavidin-BSA monolayer. Hybridization resulted in directional emission of Cy3-DNA into the prism. Additionally, the use of SPCE resulted in suppression of interfering emission from non-complementary Cy5-DNA oligomers due to weaker coupling of the more distant fluorophores with the surface plasmons. A large fraction of the total potential emission can couple to the surface plasmon resulting in improved sensitivity. We expect SPCE to have numerous applications to nucleic acid analyses.",
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DNA hybridization using surface plasmon-coupled emission. / Malicka, Joanna; Gryczynski, Ignacy; Gryczynski, Zygmunt; Lakowicz, Joseph R.

In: Proceedings of SPIE - The International Society for Optical Engineering, Vol. 5321, 27.10.2004, p. 283-288.

Research output: Contribution to journalConference article

TY - JOUR

T1 - DNA hybridization using surface plasmon-coupled emission

AU - Malicka, Joanna

AU - Gryczynski, Ignacy

AU - Gryczynski, Zygmunt

AU - Lakowicz, Joseph R.

PY - 2004/10/27

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N2 - We described a new approach to measuring DNA hybridization using surface plasmon-coupled emission (SPCE). This phenomenon occurs for fluorophores within few hundreds of nanometers of a thin metal film on a glass substrate, in our case a 50 nm thick silver film. Excited fluorophores coupled with the surface plasmons in the metal resulting in directional emission through the glass substrate. We studied the emission of Cy3-labeled DNA oligomers bound to complementary unlabeled biotinylated-oligomers, which were bound to the metal surface via a streptavidin-BSA monolayer. Hybridization resulted in directional emission of Cy3-DNA into the prism. Additionally, the use of SPCE resulted in suppression of interfering emission from non-complementary Cy5-DNA oligomers due to weaker coupling of the more distant fluorophores with the surface plasmons. A large fraction of the total potential emission can couple to the surface plasmon resulting in improved sensitivity. We expect SPCE to have numerous applications to nucleic acid analyses.

AB - We described a new approach to measuring DNA hybridization using surface plasmon-coupled emission (SPCE). This phenomenon occurs for fluorophores within few hundreds of nanometers of a thin metal film on a glass substrate, in our case a 50 nm thick silver film. Excited fluorophores coupled with the surface plasmons in the metal resulting in directional emission through the glass substrate. We studied the emission of Cy3-labeled DNA oligomers bound to complementary unlabeled biotinylated-oligomers, which were bound to the metal surface via a streptavidin-BSA monolayer. Hybridization resulted in directional emission of Cy3-DNA into the prism. Additionally, the use of SPCE resulted in suppression of interfering emission from non-complementary Cy5-DNA oligomers due to weaker coupling of the more distant fluorophores with the surface plasmons. A large fraction of the total potential emission can couple to the surface plasmon resulting in improved sensitivity. We expect SPCE to have numerous applications to nucleic acid analyses.

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