Abstract
We described a new approach to measuring DNA hybridization using surface plasmon-coupled emission (SPCE). This phenomenon occurs for fluorophores within few hundreds of nanometers of a thin metal film on a glass substrate, in our case a 50 nm thick silver film. Excited fluorophores coupled with the surface plasmons in the metal resulting in directional emission through the glass substrate. We studied the emission of Cy3-labeled DNA oligomers bound to complementary unlabeled biotinylated-oligomers, which were bound to the metal surface via a streptavidin-BSA monolayer. Hybridization resulted in directional emission of Cy3-DNA into the prism. Additionally, the use of SPCE resulted in suppression of interfering emission from non-complementary Cy5-DNA oligomers due to weaker coupling of the more distant fluorophores with the surface plasmons. A large fraction of the total potential emission can couple to the surface plasmon resulting in improved sensitivity. We expect SPCE to have numerous applications to nucleic acid analyses.
Original language | English |
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Pages (from-to) | 283-288 |
Number of pages | 6 |
Journal | Proceedings of SPIE - The International Society for Optical Engineering |
Volume | 5321 |
DOIs | |
State | Published - 2004 |
Event | Progress in Biomedical Optics and Imaging - Biomedical Vibrational Spectroscopy and Biohazard Detection Technologies - San Jose, CA, United States Duration: 25 Jan 2004 → 27 Jan 2004 |
Keywords
- Dna hybridization
- Silver film
- Surface plasmon-coupled emission