DNA hybridization assays using metal-enhanced fluorescence

Joanna Malicka; Ignacy Gryczynski; Joseph R. Lakowicz

doi:10.1016/S0006-291X(03)00935-5

DNA hybridization assays using metal-enhanced fluorescence

Joanna Malicka, Ignacy Gryczynski, Joseph R. Lakowicz

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46 Scopus citations

Abstract

We describe a new approach to DNA hybridization assays using metal-enhanced fluorescence. Thiolated oligonucleotides were bound to silver particles on a glass substrate. Addition of a complementary fluorescein-labeled oligonucleotide resulted in a dramatic time-dependent 12-fold increase in fluorescence intensity during hybridization. Proximity to silver particles resulted in a decreased fluorescence lifetime. This effect is thought to be the result of enhanced fluorescence from fluorescein near metallic silver particles. Hybridization could thus be measured from the decay kinetics of the emission, which can be measured independently from the emission intensity. These results suggest the use of silver particles as a general approach to measure DNA hybridization as a method to increase the sensitivity of DNA detection.

Original language	English
Pages (from-to)	213-218
Number of pages	6
Journal	Biochemical and Biophysical Research Communications
Volume	306
Issue number	1
DOIs	https://doi.org/10.1016/S0006-291X(03)00935-5
State	Published - 20 Jun 2003

Keywords

DNA hybridization
Metal-enhanced fluorescence
Silver nanoparticles

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10.1016/S0006-291X(03)00935-5

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title = "DNA hybridization assays using metal-enhanced fluorescence",

abstract = "We describe a new approach to DNA hybridization assays using metal-enhanced fluorescence. Thiolated oligonucleotides were bound to silver particles on a glass substrate. Addition of a complementary fluorescein-labeled oligonucleotide resulted in a dramatic time-dependent 12-fold increase in fluorescence intensity during hybridization. Proximity to silver particles resulted in a decreased fluorescence lifetime. This effect is thought to be the result of enhanced fluorescence from fluorescein near metallic silver particles. Hybridization could thus be measured from the decay kinetics of the emission, which can be measured independently from the emission intensity. These results suggest the use of silver particles as a general approach to measure DNA hybridization as a method to increase the sensitivity of DNA detection.",

keywords = "DNA hybridization, Metal-enhanced fluorescence, Silver nanoparticles",

author = "Joanna Malicka and Ignacy Gryczynski and Lakowicz, {Joseph R.}",

note = "Funding Information: This work was supported by the NIH National Center for Research Resources, RR-08119, HG-002655, EB-000682, and EB-00981. The authors thank Dr. Zygmunt Gryczynski for taking photographs used in Fig. 3 . ",

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AU - Gryczynski, Ignacy

AU - Lakowicz, Joseph R.

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N2 - We describe a new approach to DNA hybridization assays using metal-enhanced fluorescence. Thiolated oligonucleotides were bound to silver particles on a glass substrate. Addition of a complementary fluorescein-labeled oligonucleotide resulted in a dramatic time-dependent 12-fold increase in fluorescence intensity during hybridization. Proximity to silver particles resulted in a decreased fluorescence lifetime. This effect is thought to be the result of enhanced fluorescence from fluorescein near metallic silver particles. Hybridization could thus be measured from the decay kinetics of the emission, which can be measured independently from the emission intensity. These results suggest the use of silver particles as a general approach to measure DNA hybridization as a method to increase the sensitivity of DNA detection.

AB - We describe a new approach to DNA hybridization assays using metal-enhanced fluorescence. Thiolated oligonucleotides were bound to silver particles on a glass substrate. Addition of a complementary fluorescein-labeled oligonucleotide resulted in a dramatic time-dependent 12-fold increase in fluorescence intensity during hybridization. Proximity to silver particles resulted in a decreased fluorescence lifetime. This effect is thought to be the result of enhanced fluorescence from fluorescein near metallic silver particles. Hybridization could thus be measured from the decay kinetics of the emission, which can be measured independently from the emission intensity. These results suggest the use of silver particles as a general approach to measure DNA hybridization as a method to increase the sensitivity of DNA detection.

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KW - Silver nanoparticles

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DNA hybridization assays using metal-enhanced fluorescence

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