Distribution of distances in thiopeptides by fluorescence energy transfer and frequency-domain fluorometry

Wieslaw M. Wiczk, Ignacy Gryczynski, Henryk Szmacinski, Michael L. Johnson, Marian Kruszynski, Jolanta Zboinska

Research output: Contribution to journalArticle

10 Scopus citations

Abstract

Frequency-domain fluorescence spectroscopy was employed to examine the decays of tryptophan in Boc-Trp-Met-Asp-Phe-NH2 (donor) and {A figure is presented} (donor-acceptor pair). The efficiency of energy transfer in the thiopeptide amounted to 60% The measured dispersion of fluorescence decay times was used to recover the donor-acceptor distance distribution. The parameters of the Gaussian distance distribution obtained for this peptide (r, the mean distance (9 Å); hw, the halfwidth (25 Å)) indicate the lack of a distinct favorable conformation.

Original languageEnglish
Pages (from-to)43-49
Number of pages7
JournalBiophysical Chemistry
Volume32
Issue number1
DOIs
StatePublished - Oct 1988

Keywords

  • Distance distribution
  • Energy transfer
  • Fluorescence spectroscopy
  • Frequency-domain fluorometry
  • Thiopeptide

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