Distribution of apolipoprotein A-IV between the lipoprotein and the lipoprotein-free fractions of rat plasma: Possible role of lecithin:Cholesterol acyltransferase

J. G. DeLamatre, C. A. Hoffmeier, Andras G. Lacko, P. S. Roheim

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Abstract

Plasma samples were incubated under various conditions to study the effect of in vitro incubation on apolipoprotein A-IV distribution between the lipoprotein and lipoprotein-free fractions. When plasma was fractionated immediately after bleeding, apolipoprotein A-IV was present in equal concentrations in the lipoprotein and lipoprotein-free fractions. After a 4-hr, 37°C incubation, >90% of total plasma apolipoprotein A-IV was present in the lipoprotein fraction and the percentage of plasma cholesterol present as cholesteryl ester increased from 58% to 74%. When plasma was incubated for 4 hr at 37°C in the presence of 1.5 mM 5,5-dithiobis(2-nitrobenzoic acid) (DTNB), >90% of total plasma apoA-IV was present in the lipoprotein-free fraction, whereas plasma cholesteryl ester concentration did not change. Incubating heat-inactivated plasma for 4 hr also resulted in the redistribution of apolipoprotein A-IV from the lipoprotein fraction to the lipoprotein-free fraction, concurrent with no change in cholesterol esterification. When heat-inactivated plasma was incubated in the presence of a purified lecithin:cholesterol acyltransferase preparation, cholesterol esterification was restored and apolipoprotein A-IV was redistributed from the lipoprotein-free fraction to the lipoprotein fraction in such a manner that >90% was present in the lipoprotein fraction. No changes in apolipoprotein A-I and apolipoprotein E distributions were found under any of the above conditions. Thus, the in vitro plasma incubations show that apolipoprotein A-IV can move bidirectionally between lipoprotein and lipoprotein-free fractions; the direction of this movement depends on the condition of the incubation. These data also suggest that the LCAT reaction can influence apolipoprotein A-IV distribution in the plasma.

Original languageEnglish
Pages (from-to)1578-1585
Number of pages8
JournalJournal of Lipid Research
Volume24
Issue number12
StatePublished - 1 Dec 1983

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Phosphatidylcholine-Sterol O-Acyltransferase
Lipoproteins
Rats
Plasmas
Cholesterol Esters
Esterification
Cholesterol
apolipoprotein A-IV
Hot Temperature
Nitrobenzoates
Apolipoproteins A
Dithionitrobenzoic Acid
Apolipoprotein A-I
Apolipoproteins E

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title = "Distribution of apolipoprotein A-IV between the lipoprotein and the lipoprotein-free fractions of rat plasma: Possible role of lecithin:Cholesterol acyltransferase",
abstract = "Plasma samples were incubated under various conditions to study the effect of in vitro incubation on apolipoprotein A-IV distribution between the lipoprotein and lipoprotein-free fractions. When plasma was fractionated immediately after bleeding, apolipoprotein A-IV was present in equal concentrations in the lipoprotein and lipoprotein-free fractions. After a 4-hr, 37°C incubation, >90{\%} of total plasma apolipoprotein A-IV was present in the lipoprotein fraction and the percentage of plasma cholesterol present as cholesteryl ester increased from 58{\%} to 74{\%}. When plasma was incubated for 4 hr at 37°C in the presence of 1.5 mM 5,5-dithiobis(2-nitrobenzoic acid) (DTNB), >90{\%} of total plasma apoA-IV was present in the lipoprotein-free fraction, whereas plasma cholesteryl ester concentration did not change. Incubating heat-inactivated plasma for 4 hr also resulted in the redistribution of apolipoprotein A-IV from the lipoprotein fraction to the lipoprotein-free fraction, concurrent with no change in cholesterol esterification. When heat-inactivated plasma was incubated in the presence of a purified lecithin:cholesterol acyltransferase preparation, cholesterol esterification was restored and apolipoprotein A-IV was redistributed from the lipoprotein-free fraction to the lipoprotein fraction in such a manner that >90{\%} was present in the lipoprotein fraction. No changes in apolipoprotein A-I and apolipoprotein E distributions were found under any of the above conditions. Thus, the in vitro plasma incubations show that apolipoprotein A-IV can move bidirectionally between lipoprotein and lipoprotein-free fractions; the direction of this movement depends on the condition of the incubation. These data also suggest that the LCAT reaction can influence apolipoprotein A-IV distribution in the plasma.",
author = "DeLamatre, {J. G.} and Hoffmeier, {C. A.} and Lacko, {Andras G.} and Roheim, {P. S.}",
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Distribution of apolipoprotein A-IV between the lipoprotein and the lipoprotein-free fractions of rat plasma : Possible role of lecithin:Cholesterol acyltransferase. / DeLamatre, J. G.; Hoffmeier, C. A.; Lacko, Andras G.; Roheim, P. S.

In: Journal of Lipid Research, Vol. 24, No. 12, 01.12.1983, p. 1578-1585.

Research output: Contribution to journalArticle

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T1 - Distribution of apolipoprotein A-IV between the lipoprotein and the lipoprotein-free fractions of rat plasma

T2 - Possible role of lecithin:Cholesterol acyltransferase

AU - DeLamatre, J. G.

AU - Hoffmeier, C. A.

AU - Lacko, Andras G.

AU - Roheim, P. S.

PY - 1983/12/1

Y1 - 1983/12/1

N2 - Plasma samples were incubated under various conditions to study the effect of in vitro incubation on apolipoprotein A-IV distribution between the lipoprotein and lipoprotein-free fractions. When plasma was fractionated immediately after bleeding, apolipoprotein A-IV was present in equal concentrations in the lipoprotein and lipoprotein-free fractions. After a 4-hr, 37°C incubation, >90% of total plasma apolipoprotein A-IV was present in the lipoprotein fraction and the percentage of plasma cholesterol present as cholesteryl ester increased from 58% to 74%. When plasma was incubated for 4 hr at 37°C in the presence of 1.5 mM 5,5-dithiobis(2-nitrobenzoic acid) (DTNB), >90% of total plasma apoA-IV was present in the lipoprotein-free fraction, whereas plasma cholesteryl ester concentration did not change. Incubating heat-inactivated plasma for 4 hr also resulted in the redistribution of apolipoprotein A-IV from the lipoprotein fraction to the lipoprotein-free fraction, concurrent with no change in cholesterol esterification. When heat-inactivated plasma was incubated in the presence of a purified lecithin:cholesterol acyltransferase preparation, cholesterol esterification was restored and apolipoprotein A-IV was redistributed from the lipoprotein-free fraction to the lipoprotein fraction in such a manner that >90% was present in the lipoprotein fraction. No changes in apolipoprotein A-I and apolipoprotein E distributions were found under any of the above conditions. Thus, the in vitro plasma incubations show that apolipoprotein A-IV can move bidirectionally between lipoprotein and lipoprotein-free fractions; the direction of this movement depends on the condition of the incubation. These data also suggest that the LCAT reaction can influence apolipoprotein A-IV distribution in the plasma.

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