Distance-dependent fluorescence quenching observed by frequency-domain fluorometry

Joseph R. Lakowicz, Józef Kuśba, Henryk Szmacinski, Michael L. Johnson, Ignacy Gryczynski

Research output: Contribution to journalArticlepeer-review

22 Scopus citations


We describe frequency-domain measurements of the time-dependent intensity decays of p-bis[2-(5-phenyloxazolyl)] benzene (POPOP) when collisionally quenched by carbon tetrabromide (CBr4). The frequency-domain data reveal the existence of a quenching rate constant which depends exponentially on the fluorophore-quencher separation. A distance-dependent quenching rate explains the decadesold observation of upward curvature in Stern-Volmer plots.

Original languageEnglish
Pages (from-to)455-463
Number of pages9
JournalChemical Physics Letters
Issue number5-6
StatePublished - 7 May 1993


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