Distance-dependent fluorescence quenching observed by frequency-domain fluorometry

Joseph R. Lakowicz; Józef Kuśba; Henryk Szmacinski; Michael L. Johnson; Ignacy Gryczynski

doi:10.1016/0009-2614(93)80167-N

Distance-dependent fluorescence quenching observed by frequency-domain fluorometry

Joseph R. Lakowicz, Józef Kuśba, Henryk Szmacinski, Michael L. Johnson, Ignacy Gryczynski

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Abstract

We describe frequency-domain measurements of the time-dependent intensity decays of p-bis[2-(5-phenyloxazolyl)] benzene (POPOP) when collisionally quenched by carbon tetrabromide (CBr₄). The frequency-domain data reveal the existence of a quenching rate constant which depends exponentially on the fluorophore-quencher separation. A distance-dependent quenching rate explains the decadesold observation of upward curvature in Stern-Volmer plots.

Original language	English
Pages (from-to)	455-463
Number of pages	9
Journal	Chemical Physics Letters
Volume	206
Issue number	5-6
DOIs	https://doi.org/10.1016/0009-2614(93)80167-N
State	Published - 7 May 1993

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@article{bcced3c9a8bb428a8f2e52e207484db5,

title = "Distance-dependent fluorescence quenching observed by frequency-domain fluorometry",

abstract = "We describe frequency-domain measurements of the time-dependent intensity decays of p-bis[2-(5-phenyloxazolyl)] benzene (POPOP) when collisionally quenched by carbon tetrabromide (CBr4). The frequency-domain data reveal the existence of a quenching rate constant which depends exponentially on the fluorophore-quencher separation. A distance-dependent quenching rate explains the decadesold observation of upward curvature in Stern-Volmer plots.",

author = "Lakowicz, {Joseph R.} and J{\'o}zef Ku{\'s}ba and Henryk Szmacinski and Johnson, {Michael L.} and Ignacy Gryczynski",

note = "Funding Information: This work was supportedb y a grantf rom the National Instituteso f Health, GM 3961 7with support for instrumentationfr om NIH grantsR R-0751 0a nd the National Science Foundation grant DIR-8710401.",

year = "1993",

month = may,

day = "7",

doi = "10.1016/0009-2614(93)80167-N",

language = "English",

volume = "206",

pages = "455--463",

journal = "Chemical Physics Letters",

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T1 - Distance-dependent fluorescence quenching observed by frequency-domain fluorometry

AU - Lakowicz, Joseph R.

AU - Kuśba, Józef

AU - Szmacinski, Henryk

AU - Johnson, Michael L.

AU - Gryczynski, Ignacy

N1 - Funding Information: This work was supportedb y a grantf rom the National Instituteso f Health, GM 3961 7with support for instrumentationfr om NIH grantsR R-0751 0a nd the National Science Foundation grant DIR-8710401.

PY - 1993/5/7

Y1 - 1993/5/7

N2 - We describe frequency-domain measurements of the time-dependent intensity decays of p-bis[2-(5-phenyloxazolyl)] benzene (POPOP) when collisionally quenched by carbon tetrabromide (CBr4). The frequency-domain data reveal the existence of a quenching rate constant which depends exponentially on the fluorophore-quencher separation. A distance-dependent quenching rate explains the decadesold observation of upward curvature in Stern-Volmer plots.

AB - We describe frequency-domain measurements of the time-dependent intensity decays of p-bis[2-(5-phenyloxazolyl)] benzene (POPOP) when collisionally quenched by carbon tetrabromide (CBr4). The frequency-domain data reveal the existence of a quenching rate constant which depends exponentially on the fluorophore-quencher separation. A distance-dependent quenching rate explains the decadesold observation of upward curvature in Stern-Volmer plots.

UR - http://www.scopus.com/inward/record.url?scp=0010182602&partnerID=8YFLogxK

U2 - 10.1016/0009-2614(93)80167-N

DO - 10.1016/0009-2614(93)80167-N

M3 - Article

AN - SCOPUS:0010182602

SN - 0009-2614

VL - 206

SP - 455

EP - 463

JO - Chemical Physics Letters

JF - Chemical Physics Letters

IS - 5-6

ER -

Distance-dependent fluorescence quenching observed by frequency-domain fluorometry

Abstract

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