Abstract
We looked for evidence that the diffusion of heavy meromyosin is modified by its interaction with actin. To be able to observe diffusion in one dimension, we electrophoresed the complex of F-actin and heavy meromyosin in agarose gels in thin capillaries. The intensity profile of the electrophoretic band of the complex showed a sharp peak, which in 1% agarose in the electric field of 17.8 V cm-1 at room temperature migrated at 3.2 cm h-1. The time evolution of the profile after the electrophoresis ended was a measure of the diffusion of heavy meromyosin. After 10 min the intensity profile of heavy meromyosin diffusing in the presence of F-actin and ATP had undergone as much change as the profile of free heavy meromyosin. Modelling of the diffusion process showed that the mean diffusion coefficient of heavy meromyosin moving over actin in the presence of ATP was 7.2×10-7 cm2 s-1 and that it was not statistically different from the diffusion coefficient of free heavy meromyosin. This data is interpreted to show that the diffusion of heavy meromyosin is not modified by its interaction with actin.
| Original language | English |
|---|---|
| Pages (from-to) | 106-116 |
| Number of pages | 11 |
| Journal | Journal of Muscle Research and Cell Motility |
| Volume | 13 |
| Issue number | 1 |
| DOIs | |
| State | Published - 1 Feb 1992 |
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Diffusion of heavy meromyosin in the presence of F-actin and ATP. / Borejdo, Julian; Burlacu, S.
In: Journal of Muscle Research and Cell Motility, Vol. 13, No. 1, 01.02.1992, p. 106-116.Research output: Contribution to journal › Article
TY - JOUR
T1 - Diffusion of heavy meromyosin in the presence of F-actin and ATP
AU - Borejdo, Julian
AU - Burlacu, S.
PY - 1992/2/1
Y1 - 1992/2/1
N2 - We looked for evidence that the diffusion of heavy meromyosin is modified by its interaction with actin. To be able to observe diffusion in one dimension, we electrophoresed the complex of F-actin and heavy meromyosin in agarose gels in thin capillaries. The intensity profile of the electrophoretic band of the complex showed a sharp peak, which in 1% agarose in the electric field of 17.8 V cm-1 at room temperature migrated at 3.2 cm h-1. The time evolution of the profile after the electrophoresis ended was a measure of the diffusion of heavy meromyosin. After 10 min the intensity profile of heavy meromyosin diffusing in the presence of F-actin and ATP had undergone as much change as the profile of free heavy meromyosin. Modelling of the diffusion process showed that the mean diffusion coefficient of heavy meromyosin moving over actin in the presence of ATP was 7.2×10-7 cm2 s-1 and that it was not statistically different from the diffusion coefficient of free heavy meromyosin. This data is interpreted to show that the diffusion of heavy meromyosin is not modified by its interaction with actin.
AB - We looked for evidence that the diffusion of heavy meromyosin is modified by its interaction with actin. To be able to observe diffusion in one dimension, we electrophoresed the complex of F-actin and heavy meromyosin in agarose gels in thin capillaries. The intensity profile of the electrophoretic band of the complex showed a sharp peak, which in 1% agarose in the electric field of 17.8 V cm-1 at room temperature migrated at 3.2 cm h-1. The time evolution of the profile after the electrophoresis ended was a measure of the diffusion of heavy meromyosin. After 10 min the intensity profile of heavy meromyosin diffusing in the presence of F-actin and ATP had undergone as much change as the profile of free heavy meromyosin. Modelling of the diffusion process showed that the mean diffusion coefficient of heavy meromyosin moving over actin in the presence of ATP was 7.2×10-7 cm2 s-1 and that it was not statistically different from the diffusion coefficient of free heavy meromyosin. This data is interpreted to show that the diffusion of heavy meromyosin is not modified by its interaction with actin.
UR - http://www.scopus.com/inward/record.url?scp=0026603346&partnerID=8YFLogxK
U2 - 10.1007/BF01738434
DO - 10.1007/BF01738434
M3 - Article
C2 - 1556166
AN - SCOPUS:0026603346
VL - 13
SP - 106
EP - 116
JO - Journal of Muscle Research and Cell Motility
JF - Journal of Muscle Research and Cell Motility
SN - 0142-4319
IS - 1
ER -