Differential role for NMDA and non-nmda receptor subtypes in baroreceptor afferent integration in rat NTS

Jing Zhang, Steve Wayne Mifflin

Research output: Contribution to journalArticle

2 Scopus citations

Abstract

The role of NMDA and non-NMDA receptor subtypes in baroreccptor afferent integration in NTS was investigated in pentoharbital anesthetized, paralyzed and artificially ventilated rats. Aortic nerve (AN) evoked discharge was classified as monosynaptic (MS; second of two stimuli separated by 5 ms evoked discharge; minimal onset latency variability) or polysynaptic (PS). The effects of iontophoretic application of the NMDA antagonist AP-5 (lOmM) or the non-NMDA antagonists CNQX (5mM) or NBQX (4mM) on single pulse AN evoked discharge and spontaneous discharge were examined using ejecting currents of 20&40nA. Ai these "doses" AP-5 blocked NMDA induced excitation but not that induced by AMPA or kainic acid co-iontophoresis while CNQX and NBQX blocked AMPA and kainic acid induced excitation but not that induced by NMDA. AP-5 inhibited PS AN inputs (MANOVA, p<.(K)5, n=12), but not MS AN inputs (p=. 15, n=6). In contrast to AP-5, CNQX and NBQX inhibited both MS (p<.05 for both drugs, n=6&8 respectively) and PS (p<.fK)5 for both drugs, n=ll&13 respectively) evoked AN inputs. The spontaneous discharge of AN evoked cells was significantly inhibited by AP-5 (p<. 01. n=2 MS & 7 PS), CNQX (p<.01, n=2 MS & 10 PS) and NBQX (p<.(X)l. n=5 MS & 9 PS). The data suggest that excitatory amino acid receptor subtypes are differentially involved in the integration of MS and PS baroreceptor inputs by NTS neurons.

Original languageEnglish
JournalFASEB Journal
Volume10
Issue number3
StatePublished - 1 Dec 1996

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