TY - JOUR
T1 - Differential proliferative index of cancer stem-like cells in primary and recurrent medulloblastoma in human
AU - Tang, Xuqun
AU - Yao, Yu
AU - Zhu, Jingjing
AU - Jin, Kunlin
AU - Wang, Yin
AU - Mao, Ying
AU - Zhou, Liangfu
N1 - Funding Information:
Acknowledgments This work was supported by the National Natural Science Foundation of China grant 30872655 (to Y. M) and 81025013 (to Y. M), The Project for Science and Technology Commission of Shanghai Municipality grant 10JC1402200 (to L.F. Z) and 08411953600 (to Y. M), The Project for National 985 Engineering of China (to Y. M), and the “Dawn Tracking” Program of Shanghai Education Commission, China Grant 10GG01 (to Y. M). We thank Dr. An Sun, Qiwu Xu, and Ping Zhong for assistance on surgery.
PY - 2012/11
Y1 - 2012/11
N2 - Purpose Medulloblastoma is the most common malignant primitive neuroectodermal tumor found in children. It has a tendency to recur at a primary or distant site. The mechanism underlying the regulation of the recurrence of medulloblastoma remains largely unknown. Recently, several reports have described that cancer stem-like cells (CSCs) can be identified and isolated in medulloblastoma. The authors therefore attempted to demonstrate the correlation between the biological features of medulloblastoma's CSCs and its recurrence Methods The data used were obtained in five consecutive patients with medulloblastomas who subsequently experienced tumor recurrence from 2004 to 2007. The authors performed the immunohistochemical assays to analyze the expression of CSC markers, proliferation features, and proliferative status of CSCs in primary and recurrent medulloblastoma. Results Of the five patients, two had recurrence at the primary site and three had a distant recurrence. CSC markers such as CD133(Prominin-1), DCX, PSA-NCAM, TUC-4, and nestin were expressed regardless of primary or recurrent medulloblastoma. All the five tumor specimens had a high proliferation index (PI). The PI was even higher in the group of patients after recurrence at a distant site (p<0.05), while the PI remained almost the same after primary recurrence. The Ki67/nestin-, Ki67/DCX-, and Ki67/TUC-4-positive cells were significantly increased in recurrent medulloblastoma at both the primary and distant sites, whereas CSCs in primary medulloblastoma showed much lower proliferative features (p<0.05). Conclusions Our data suggest that tumorigenesis of medulloblastomas and their recurrence might be related to CSCs. More proliferating CSCs in medulloblastomas denote worse prognosis.
AB - Purpose Medulloblastoma is the most common malignant primitive neuroectodermal tumor found in children. It has a tendency to recur at a primary or distant site. The mechanism underlying the regulation of the recurrence of medulloblastoma remains largely unknown. Recently, several reports have described that cancer stem-like cells (CSCs) can be identified and isolated in medulloblastoma. The authors therefore attempted to demonstrate the correlation between the biological features of medulloblastoma's CSCs and its recurrence Methods The data used were obtained in five consecutive patients with medulloblastomas who subsequently experienced tumor recurrence from 2004 to 2007. The authors performed the immunohistochemical assays to analyze the expression of CSC markers, proliferation features, and proliferative status of CSCs in primary and recurrent medulloblastoma. Results Of the five patients, two had recurrence at the primary site and three had a distant recurrence. CSC markers such as CD133(Prominin-1), DCX, PSA-NCAM, TUC-4, and nestin were expressed regardless of primary or recurrent medulloblastoma. All the five tumor specimens had a high proliferation index (PI). The PI was even higher in the group of patients after recurrence at a distant site (p<0.05), while the PI remained almost the same after primary recurrence. The Ki67/nestin-, Ki67/DCX-, and Ki67/TUC-4-positive cells were significantly increased in recurrent medulloblastoma at both the primary and distant sites, whereas CSCs in primary medulloblastoma showed much lower proliferative features (p<0.05). Conclusions Our data suggest that tumorigenesis of medulloblastomas and their recurrence might be related to CSCs. More proliferating CSCs in medulloblastomas denote worse prognosis.
KW - Cancer stem-like cells
KW - Medulloblastoma
KW - Prognosis
KW - Proliferation
KW - Recurrence
UR - http://www.scopus.com/inward/record.url?scp=84867583097&partnerID=8YFLogxK
U2 - 10.1007/s00381-012-1856-z
DO - 10.1007/s00381-012-1856-z
M3 - Article
C2 - 22814953
AN - SCOPUS:84867583097
SN - 0256-7040
VL - 28
SP - 1869
EP - 1877
JO - Child's Nervous System
JF - Child's Nervous System
IS - 11
ER -