TY - JOUR
T1 - Differential expression of annexins I and II in bovine bronchial epithelial cells.
AU - Vishwanatha, J. K.
AU - Muns, G.
AU - Beckmann, J. D.
AU - Davis, R. G.
AU - Rubinstein, I.
PY - 1995/3
Y1 - 1995/3
N2 - Annexins I and II have been proposed to participate in regulation of inflammation and cell proliferation. In the present study, we examined the expression of annexins I and II in various levels of the bovine respiratory tract and in cultured bovine bronchial epithelial cells. Immunoblot analysis of whole-tissue extracts revealed low-level expression of annexins I and II in bronchial mucosa mainstem to fourth generation. In contrast, high levels of annexins I and II (15- to 20-fold higher than in the bronchial mucosa) were seen in the distal lung parenchyma. Immunohistochemical staining of tracheobronchial tissue sections with anti-annexin I antibody revealed an uneven positive reaction of about 30 to 40% of the columnar differentiated ciliated and secretory cells, with no obvious positivity within the nondifferentiated basal epithelial cell layer. In contrast, anti-annexin II antibody reacted nearly uniformly within the basal cell layer, with no obvious decoration of the differentiated cell types. These results were confirmed by immunoblot analysis of annexin I and II in density fractionated populations of basal and secretory/ciliated epithelial cells. Both annexins I and II were expressed at constitutive levels in bovine bronchial epithelial cells grown in glucocorticoid- and serum-free medium, and dexamethasone increased expression of both proteins in a concentration-dependent manner. We conclude that annexins I and II are differentially expressed in the differentiated ciliated cells and undifferentiated basal cells.(ABSTRACT TRUNCATED AT 250 WORDS)
AB - Annexins I and II have been proposed to participate in regulation of inflammation and cell proliferation. In the present study, we examined the expression of annexins I and II in various levels of the bovine respiratory tract and in cultured bovine bronchial epithelial cells. Immunoblot analysis of whole-tissue extracts revealed low-level expression of annexins I and II in bronchial mucosa mainstem to fourth generation. In contrast, high levels of annexins I and II (15- to 20-fold higher than in the bronchial mucosa) were seen in the distal lung parenchyma. Immunohistochemical staining of tracheobronchial tissue sections with anti-annexin I antibody revealed an uneven positive reaction of about 30 to 40% of the columnar differentiated ciliated and secretory cells, with no obvious positivity within the nondifferentiated basal epithelial cell layer. In contrast, anti-annexin II antibody reacted nearly uniformly within the basal cell layer, with no obvious decoration of the differentiated cell types. These results were confirmed by immunoblot analysis of annexin I and II in density fractionated populations of basal and secretory/ciliated epithelial cells. Both annexins I and II were expressed at constitutive levels in bovine bronchial epithelial cells grown in glucocorticoid- and serum-free medium, and dexamethasone increased expression of both proteins in a concentration-dependent manner. We conclude that annexins I and II are differentially expressed in the differentiated ciliated cells and undifferentiated basal cells.(ABSTRACT TRUNCATED AT 250 WORDS)
UR - http://www.scopus.com/inward/record.url?scp=0029269354&partnerID=8YFLogxK
U2 - 10.1165/ajrcmb.12.3.7873194
DO - 10.1165/ajrcmb.12.3.7873194
M3 - Article
C2 - 7873194
AN - SCOPUS:0029269354
SN - 1044-1549
VL - 12
SP - 280
EP - 286
JO - American Journal of Respiratory Cell and Molecular Biology
JF - American Journal of Respiratory Cell and Molecular Biology
IS - 3
ER -