TY - JOUR
T1 - Differential effects of nerve growth factor on expression of dopamine 2 receptor subtypes in GH3 rat pituitary tumor cells
AU - Su, Zhipeng
AU - Jiang, Xiaolong
AU - Wang, Chengde
AU - Liu, Jie
AU - Chen, Yunxiang
AU - Li, Qun
AU - Wu, Jinsen
AU - Zheng, Weiming
AU - Zhuge, Qichuan
AU - Jin, Kunlin
AU - Wu, Zhebao
N1 - Funding Information:
Acknowledgments This project was supported by grants from the National Natural Science Foundation of China (30800347), Zhejiang Provincial Natural Science Foundation (R2091137, Y2110554), Zhejiang Provincial Program for the Cultivation of High-level Innovative Health talents, Science and Technology Bureau of Wenzhou City (Y20080226, H20070040).
PY - 2012/12
Y1 - 2012/12
N2 - Nerve growth factor (NGF) can increase expression of dopamine 2 receptors (D2R) in GH3 rat pituitary tumor cells (GH3 cells). As D2R exists in long (D2L) and short (D2S) isoforms, effects of NGF on D2R subtypes have not been accurately evaluated. In this study, we compared mRNA levels of D2R subtypes in GH3 cells treated with or without NGF with real-time RT-PCR. In addition, we also evaluated the relationship between GH3 cell growth after bromocriptine treatment and mRNA levels of D2R subtypes. We found that D2R total, D2L, and D2S mRNA in GH3 cells were significantly increased after NGF treatment, compared with the vehicle group. Moreover, NGF increased the ratio of D2S to D2L. GH3 cell survival rate after bromocriptine treatment was negatively correlated with D2R total mRNA, and D2S may be more potent than D2L in inhibiting cell growth. Cell apoptosis rate was highly elevated in GH3 cells treated with NGF and bromocriptine, compared with the control group or the group treated with NGF or bromocriptine alone. Our data provide preliminary evidence that the effect of NGF was more prominent on expression of D2S than D2L, in addition, D2S might have a greater impact suppressing GH3 cells growth than D2L.
AB - Nerve growth factor (NGF) can increase expression of dopamine 2 receptors (D2R) in GH3 rat pituitary tumor cells (GH3 cells). As D2R exists in long (D2L) and short (D2S) isoforms, effects of NGF on D2R subtypes have not been accurately evaluated. In this study, we compared mRNA levels of D2R subtypes in GH3 cells treated with or without NGF with real-time RT-PCR. In addition, we also evaluated the relationship between GH3 cell growth after bromocriptine treatment and mRNA levels of D2R subtypes. We found that D2R total, D2L, and D2S mRNA in GH3 cells were significantly increased after NGF treatment, compared with the vehicle group. Moreover, NGF increased the ratio of D2S to D2L. GH3 cell survival rate after bromocriptine treatment was negatively correlated with D2R total mRNA, and D2S may be more potent than D2L in inhibiting cell growth. Cell apoptosis rate was highly elevated in GH3 cells treated with NGF and bromocriptine, compared with the control group or the group treated with NGF or bromocriptine alone. Our data provide preliminary evidence that the effect of NGF was more prominent on expression of D2S than D2L, in addition, D2S might have a greater impact suppressing GH3 cells growth than D2L.
KW - Dopamine 2 receptor
KW - Dopamine receptor agonist
KW - GH3 cell
KW - Nerve growth factor
UR - http://www.scopus.com/inward/record.url?scp=84878227070&partnerID=8YFLogxK
U2 - 10.1007/s12020-012-9715-y
DO - 10.1007/s12020-012-9715-y
M3 - Article
C2 - 22684586
AN - SCOPUS:84878227070
SN - 1355-008X
VL - 42
SP - 670
EP - 675
JO - Endocrine
JF - Endocrine
IS - 3
ER -