TY - JOUR
T1 - Dietary fat modulates serum paraoxonase 1 activity in rats
AU - Kudchodkar, Bhalchandra J.
AU - Lacko, Andras G.
AU - Dory, Ladislav
AU - Fungwe, Thomas V.
PY - 2000
Y1 - 2000
N2 - We examined the effects of dietary fats with specific fatty acid compositions, on serum paraoxonase (PON1) activity in rats. Male adult Sprague-Dawley rats were divided randomly into four dietary groups. One group received the control diet [AIN 93M with soybean oil (5 g/100 g diet)], whereas the remaining three groups received the modified control diet supplemented with (15 g/100 g diet) triolein, tripalmitin or fish oil, respectively. After 20 d, blood was obtained after overnight food deprivation and PON1 activity was determined. Serum lipids and lipid components of lipoproteins were also determined. Serum PON1 activity [μmol/(L·min)] was significantly (P < 0.05) higher in triolein (98 ± 6) and lower in fish oil (41 ± 4), compared with tripalmitin-fed rats (63 ± 11). Serum PON1 activity in tripalmitin-fed rats was comparable to that of controls (67 ± 9). Serum PON1 activity correlated significantly with serum lecithin: cholesterol acyltransferase (LCAT) activity (r = 0.77, P < 0.001) and was transported in blood principally in association with the denser subfraction of HDL, very high density lipoprotein (VHDL; d > 1.15 kg/L). Serum PON1 activity correlated strongly with serum lipids as well as lipids of VLDL, HDL and its subfractions. Multiple linear regression analysis, however, showed a significant relationship of serum PON1 activity, principally with the phospholipids of VHDL (r = 0.47, P < 0.002). These data suggest that the modulation of serum PON1 activity by dietary fat may be mediated via the effect of the specific fatty acids on the synthesis and secretion of VHDL, the subfraction of HDL that transports the majority of PON1 in the blood.
AB - We examined the effects of dietary fats with specific fatty acid compositions, on serum paraoxonase (PON1) activity in rats. Male adult Sprague-Dawley rats were divided randomly into four dietary groups. One group received the control diet [AIN 93M with soybean oil (5 g/100 g diet)], whereas the remaining three groups received the modified control diet supplemented with (15 g/100 g diet) triolein, tripalmitin or fish oil, respectively. After 20 d, blood was obtained after overnight food deprivation and PON1 activity was determined. Serum lipids and lipid components of lipoproteins were also determined. Serum PON1 activity [μmol/(L·min)] was significantly (P < 0.05) higher in triolein (98 ± 6) and lower in fish oil (41 ± 4), compared with tripalmitin-fed rats (63 ± 11). Serum PON1 activity in tripalmitin-fed rats was comparable to that of controls (67 ± 9). Serum PON1 activity correlated significantly with serum lecithin: cholesterol acyltransferase (LCAT) activity (r = 0.77, P < 0.001) and was transported in blood principally in association with the denser subfraction of HDL, very high density lipoprotein (VHDL; d > 1.15 kg/L). Serum PON1 activity correlated strongly with serum lipids as well as lipids of VLDL, HDL and its subfractions. Multiple linear regression analysis, however, showed a significant relationship of serum PON1 activity, principally with the phospholipids of VHDL (r = 0.47, P < 0.002). These data suggest that the modulation of serum PON1 activity by dietary fat may be mediated via the effect of the specific fatty acids on the synthesis and secretion of VHDL, the subfraction of HDL that transports the majority of PON1 in the blood.
KW - Dietary fat
KW - Lipoproteins
KW - Paraoxonase 1
KW - Rats
UR - http://www.scopus.com/inward/record.url?scp=0033803134&partnerID=8YFLogxK
U2 - 10.1093/jn/130.10.2427
DO - 10.1093/jn/130.10.2427
M3 - Article
C2 - 11015468
AN - SCOPUS:0033803134
SN - 0022-3166
VL - 130
SP - 2427
EP - 2433
JO - Journal of Nutrition
JF - Journal of Nutrition
IS - 10
ER -