TY - JOUR
T1 - Developmental validation of VeriFiler™ Plus PCR Amplification Kit
T2 - A 6-dye multiplex assay designed for casework samples
AU - Green, Robert
AU - Elliott, Jennifer L.
AU - Norona, Wilma
AU - Go, Francine
AU - Nguyen, Vivian T.
AU - Ge, Jianye
AU - Short, Marc L.
AU - Mulero, Julio J.
AU - Zhong, Chang
N1 - Funding Information:
The authors gratefully acknowledge the following laboratories for beta-testing the VeriFiler Plus Kit: Adrian Linacre's Lab at Flinders University, State Forensic Examination Committee of the Republic of Belarus, the Argentine Forensic Anthropology Team, Instituto de Criminalística de São Paulo, Brazil, Carolyn Steffen in National Institute of Standard and Technology, US. The authors also wish to extend our gratitude to Ying Wang, Wenting Luo, Christina Buchanan-Wright, and the members of the Human Identification R&D and Product Marketing groups at Thermo Fisher Scientific for technical assistance and valuable comments on this work.
Publisher Copyright:
© 2021 The Authors
PY - 2021/7
Y1 - 2021/7
N2 - The VeriFiler™ Plus PCR Amplification Kit is a 6-dye multiplex assay that simultaneously amplifies a set of 23 autosomal markers (D3S1358, vWA, D16S539, CSF1PO, D6S1043, D8S1179, D21S11, D18S51, D5S818, D2S441, D19S433, FGA, D10S1248, D22S1045, D1S1656, D13S317, D7S820, Penta E, Penta D, TH01, D12S391, D2S1338, and TPOX), a quality indicator system, and two sex-identification markers. Combined, the markers satisfy the requirements of the Chinese National autosomal DNA database as well as expanded CODIS (Combined DNA Index System). The VeriFiler Plus kit was developed with an improved Master Mix which incorporates the brighter TED™ dye, and accommodates a higher sample loading volume thus allowing for increased sensitivity and enabling maximum information recovery from challenging casework samples including touch, degraded, and inhibited samples. Here, we report the results of the developmental validation study which followed the SWGDAM (Scientific Working Group on DNA Analysis Methods) guidelines and includes data for PCR-based studies, sensitivity, species specificity, stability, precision, reproducibility and repeatability, concordance, stutter, DNA mixtures, and performance on mock casework samples. The results validate the multiplex design as well as demonstrate the kit's robustness, reliability, and suitability as an assay for human identification with casework DNA samples.
AB - The VeriFiler™ Plus PCR Amplification Kit is a 6-dye multiplex assay that simultaneously amplifies a set of 23 autosomal markers (D3S1358, vWA, D16S539, CSF1PO, D6S1043, D8S1179, D21S11, D18S51, D5S818, D2S441, D19S433, FGA, D10S1248, D22S1045, D1S1656, D13S317, D7S820, Penta E, Penta D, TH01, D12S391, D2S1338, and TPOX), a quality indicator system, and two sex-identification markers. Combined, the markers satisfy the requirements of the Chinese National autosomal DNA database as well as expanded CODIS (Combined DNA Index System). The VeriFiler Plus kit was developed with an improved Master Mix which incorporates the brighter TED™ dye, and accommodates a higher sample loading volume thus allowing for increased sensitivity and enabling maximum information recovery from challenging casework samples including touch, degraded, and inhibited samples. Here, we report the results of the developmental validation study which followed the SWGDAM (Scientific Working Group on DNA Analysis Methods) guidelines and includes data for PCR-based studies, sensitivity, species specificity, stability, precision, reproducibility and repeatability, concordance, stutter, DNA mixtures, and performance on mock casework samples. The results validate the multiplex design as well as demonstrate the kit's robustness, reliability, and suitability as an assay for human identification with casework DNA samples.
KW - CODIS
KW - Casework samples
KW - Chinese National Database
KW - Forensic validation studies
KW - Human identification
KW - Multiplex PCR assay
KW - STR
KW - VeriFiler™ Plus Kit
UR - http://www.scopus.com/inward/record.url?scp=85102564142&partnerID=8YFLogxK
U2 - 10.1016/j.fsigen.2021.102494
DO - 10.1016/j.fsigen.2021.102494
M3 - Article
C2 - 33740707
AN - SCOPUS:85102564142
SN - 1872-4973
VL - 53
JO - Forensic Science International: Genetics
JF - Forensic Science International: Genetics
M1 - 102494
ER -