TY - JOUR
T1 - Developmental validation of the Huaxia™ Platinum PCR amplification kit
T2 - A 6-dye multiplex direct amplification assay designed for Chinese reference samples
AU - Zhong, C.
AU - Gopinath, Siddhita
AU - Norona, W.
AU - Ge, Jianye
AU - Lagacé, Robert E.
AU - Wang, Dennis Y.
AU - Short, Marc L.
AU - Mulero, Julio J.
N1 - Funding Information:
The authors gratefully acknowledge the following laboratories for beta-testing the Huaxia™ Platinum Kit: Huaxi Medical Center, Ningxia PSB, etc. The authors also wish to extend our gratitude to Ying Wang, Yu Liang, and the members of the Human Identification R&D and Product Marketing groups at Thermo Fisher Scientific for technical assistance and valuable comments on this work.
Publisher Copyright:
© 2019 Thermo Fisher Scientific
PY - 2019/9
Y1 - 2019/9
N2 - The Huaxia™ Platinum Kit for short tandem repeat (STR) amplification was designed to meet the needs of the rapidly growing Chinese forensic database. This PCR multiplex allows simultaneous amplification of the following autosomal loci: D3S1358, vWA, D16S539, CSF1PO, TPOX, D8S1179, D21S11, D18S51, Penta E, D2S441, D19S433, TH01, FGA, D22S1045, D5S818, D13S317, D7S820, D6S1043, D10S1248, D1S1656, D12S391, D2S1338, Penta D and the gender-identification markers Yindel, and AMEL. The Huaxia™ Platinum Kit enables direct amplification from blood and buccal samples stored on treated and untreated paper, and features an optimized PCR protocol that yields time to results in less than 45 min. Developmental validation testing followed SWGDAM guidelines and demonstrated that this assay produces reproducible and accurate results. Studies on 798 individuals in 4 major Chinese ethnic groups produced highly concordant results with other commercially available STR genotyping kits. The validation results demonstrate that the Huaxia™ Platinum Kit is a robust and reliable identification system for forensic DNA databasing applications.
AB - The Huaxia™ Platinum Kit for short tandem repeat (STR) amplification was designed to meet the needs of the rapidly growing Chinese forensic database. This PCR multiplex allows simultaneous amplification of the following autosomal loci: D3S1358, vWA, D16S539, CSF1PO, TPOX, D8S1179, D21S11, D18S51, Penta E, D2S441, D19S433, TH01, FGA, D22S1045, D5S818, D13S317, D7S820, D6S1043, D10S1248, D1S1656, D12S391, D2S1338, Penta D and the gender-identification markers Yindel, and AMEL. The Huaxia™ Platinum Kit enables direct amplification from blood and buccal samples stored on treated and untreated paper, and features an optimized PCR protocol that yields time to results in less than 45 min. Developmental validation testing followed SWGDAM guidelines and demonstrated that this assay produces reproducible and accurate results. Studies on 798 individuals in 4 major Chinese ethnic groups produced highly concordant results with other commercially available STR genotyping kits. The validation results demonstrate that the Huaxia™ Platinum Kit is a robust and reliable identification system for forensic DNA databasing applications.
KW - Direct amplification
KW - Forensic validation studies
KW - Huaxia™ Platinum Kit
KW - Rapid identification
KW - STR
UR - http://www.scopus.com/inward/record.url?scp=85069918365&partnerID=8YFLogxK
U2 - 10.1016/j.fsigen.2019.07.001
DO - 10.1016/j.fsigen.2019.07.001
M3 - Article
C2 - 31374458
AN - SCOPUS:85069918365
SN - 1872-4973
VL - 42
SP - 190
EP - 197
JO - Forensic Science International: Genetics
JF - Forensic Science International: Genetics
ER -