Developmental validation of the EX20+4 system

Shuanglin Li, Chao Liu, Hong Liu, Jianye Ge, Bruce Budowle, Changhui Liu, Weiguo Zheng, Fayuan Li, Binwen Ge

Research output: Contribution to journalArticle

4 Scopus citations

Abstract

The EX20+4Y System is a polymerase chain reaction (PCR)-based amplification kit that enables typing of 19 autosomal short tandem repeat (STR) loci (i.e., CSF1PO, D13S317, D16S539, D18S51, D21S11, D3S1358, D5S818, D7S820, D8S1179, FGA, TH01, TPOX, vWA, Penta D, Penta E, D2S1338, D19S433, D12S391, D6S1043), four widely used Y chromosome-specific STR (Y-STR) loci (DYS458, DYS456, DYS391, DYS635), and amelogenin. In this study, this multiplex system was validated for sensitivity of detection, DNA mixtures, inhibitor tolerance, species specificity based on the Scientific Working Group on DNA Analysis methods (SWGDAM) developmental validation guidelines, and the Chinese criteria for the human fluorescent STR multiplex PCR reagent. The results show that the EX20+4 System is a robust and reliable amplification kit which can be used for human identification testing.

Original languageEnglish
Pages (from-to)207-213
Number of pages7
JournalForensic Science International: Genetics
Volume11
Issue number1
DOIs
StatePublished - Jul 2014

Keywords

  • DNA typing
  • Developmental validation
  • Forensic science
  • Multiplexing
  • Short tandem repeat (STR)

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