Developmental validation of the EX20+4 system

Shuanglin Li, Chao Liu, Hong Liu, Jianye Ge, Bruce Budowle, Changhui Liu, Weiguo Zheng, Fayuan Li, Binwen Ge

Research output: Contribution to journalArticle

4 Citations (Scopus)

Abstract

The EX20+4Y System is a polymerase chain reaction (PCR)-based amplification kit that enables typing of 19 autosomal short tandem repeat (STR) loci (i.e., CSF1PO, D13S317, D16S539, D18S51, D21S11, D3S1358, D5S818, D7S820, D8S1179, FGA, TH01, TPOX, vWA, Penta D, Penta E, D2S1338, D19S433, D12S391, D6S1043), four widely used Y chromosome-specific STR (Y-STR) loci (DYS458, DYS456, DYS391, DYS635), and amelogenin. In this study, this multiplex system was validated for sensitivity of detection, DNA mixtures, inhibitor tolerance, species specificity based on the Scientific Working Group on DNA Analysis methods (SWGDAM) developmental validation guidelines, and the Chinese criteria for the human fluorescent STR multiplex PCR reagent. The results show that the EX20+4 System is a robust and reliable amplification kit which can be used for human identification testing.

Original languageEnglish
Pages (from-to)207-213
Number of pages7
JournalForensic Science International: Genetics
Volume11
Issue number1
DOIs
StatePublished - Jul 2014

Fingerprint

Microsatellite Repeats
Rubiaceae
Amelogenin
Species Specificity
Forensic Anthropology
Y Chromosome
Multiplex Polymerase Chain Reaction
DNA
Guidelines
Polymerase Chain Reaction

Keywords

  • DNA typing
  • Developmental validation
  • Forensic science
  • Multiplexing
  • Short tandem repeat (STR)

Cite this

Li, Shuanglin ; Liu, Chao ; Liu, Hong ; Ge, Jianye ; Budowle, Bruce ; Liu, Changhui ; Zheng, Weiguo ; Li, Fayuan ; Ge, Binwen. / Developmental validation of the EX20+4 system. In: Forensic Science International: Genetics. 2014 ; Vol. 11, No. 1. pp. 207-213.
@article{2a68bd6c4c934ebeaf4c2a2685b2274a,
title = "Developmental validation of the EX20+4 system",
abstract = "The EX20+4Y System is a polymerase chain reaction (PCR)-based amplification kit that enables typing of 19 autosomal short tandem repeat (STR) loci (i.e., CSF1PO, D13S317, D16S539, D18S51, D21S11, D3S1358, D5S818, D7S820, D8S1179, FGA, TH01, TPOX, vWA, Penta D, Penta E, D2S1338, D19S433, D12S391, D6S1043), four widely used Y chromosome-specific STR (Y-STR) loci (DYS458, DYS456, DYS391, DYS635), and amelogenin. In this study, this multiplex system was validated for sensitivity of detection, DNA mixtures, inhibitor tolerance, species specificity based on the Scientific Working Group on DNA Analysis methods (SWGDAM) developmental validation guidelines, and the Chinese criteria for the human fluorescent STR multiplex PCR reagent. The results show that the EX20+4 System is a robust and reliable amplification kit which can be used for human identification testing.",
keywords = "DNA typing, Developmental validation, Forensic science, Multiplexing, Short tandem repeat (STR)",
author = "Shuanglin Li and Chao Liu and Hong Liu and Jianye Ge and Bruce Budowle and Changhui Liu and Weiguo Zheng and Fayuan Li and Binwen Ge",
year = "2014",
month = "7",
doi = "10.1016/j.fsigen.2014.03.001",
language = "English",
volume = "11",
pages = "207--213",
journal = "Forensic Science International: Genetics",
issn = "1872-4973",
publisher = "Elsevier Ireland Ltd",
number = "1",

}

Li, S, Liu, C, Liu, H, Ge, J, Budowle, B, Liu, C, Zheng, W, Li, F & Ge, B 2014, 'Developmental validation of the EX20+4 system', Forensic Science International: Genetics, vol. 11, no. 1, pp. 207-213. https://doi.org/10.1016/j.fsigen.2014.03.001

Developmental validation of the EX20+4 system. / Li, Shuanglin; Liu, Chao; Liu, Hong; Ge, Jianye; Budowle, Bruce; Liu, Changhui; Zheng, Weiguo; Li, Fayuan; Ge, Binwen.

In: Forensic Science International: Genetics, Vol. 11, No. 1, 07.2014, p. 207-213.

Research output: Contribution to journalArticle

TY - JOUR

T1 - Developmental validation of the EX20+4 system

AU - Li, Shuanglin

AU - Liu, Chao

AU - Liu, Hong

AU - Ge, Jianye

AU - Budowle, Bruce

AU - Liu, Changhui

AU - Zheng, Weiguo

AU - Li, Fayuan

AU - Ge, Binwen

PY - 2014/7

Y1 - 2014/7

N2 - The EX20+4Y System is a polymerase chain reaction (PCR)-based amplification kit that enables typing of 19 autosomal short tandem repeat (STR) loci (i.e., CSF1PO, D13S317, D16S539, D18S51, D21S11, D3S1358, D5S818, D7S820, D8S1179, FGA, TH01, TPOX, vWA, Penta D, Penta E, D2S1338, D19S433, D12S391, D6S1043), four widely used Y chromosome-specific STR (Y-STR) loci (DYS458, DYS456, DYS391, DYS635), and amelogenin. In this study, this multiplex system was validated for sensitivity of detection, DNA mixtures, inhibitor tolerance, species specificity based on the Scientific Working Group on DNA Analysis methods (SWGDAM) developmental validation guidelines, and the Chinese criteria for the human fluorescent STR multiplex PCR reagent. The results show that the EX20+4 System is a robust and reliable amplification kit which can be used for human identification testing.

AB - The EX20+4Y System is a polymerase chain reaction (PCR)-based amplification kit that enables typing of 19 autosomal short tandem repeat (STR) loci (i.e., CSF1PO, D13S317, D16S539, D18S51, D21S11, D3S1358, D5S818, D7S820, D8S1179, FGA, TH01, TPOX, vWA, Penta D, Penta E, D2S1338, D19S433, D12S391, D6S1043), four widely used Y chromosome-specific STR (Y-STR) loci (DYS458, DYS456, DYS391, DYS635), and amelogenin. In this study, this multiplex system was validated for sensitivity of detection, DNA mixtures, inhibitor tolerance, species specificity based on the Scientific Working Group on DNA Analysis methods (SWGDAM) developmental validation guidelines, and the Chinese criteria for the human fluorescent STR multiplex PCR reagent. The results show that the EX20+4 System is a robust and reliable amplification kit which can be used for human identification testing.

KW - DNA typing

KW - Developmental validation

KW - Forensic science

KW - Multiplexing

KW - Short tandem repeat (STR)

UR - http://www.scopus.com/inward/record.url?scp=84899997723&partnerID=8YFLogxK

U2 - 10.1016/j.fsigen.2014.03.001

DO - 10.1016/j.fsigen.2014.03.001

M3 - Article

C2 - 24815370

AN - SCOPUS:84899997723

VL - 11

SP - 207

EP - 213

JO - Forensic Science International: Genetics

JF - Forensic Science International: Genetics

SN - 1872-4973

IS - 1

ER -