TY - JOUR
T1 - Developmental validation of the EX20+4 system
AU - Li, Shuanglin
AU - Liu, Chao
AU - Liu, Hong
AU - Ge, Jianye
AU - Budowle, Bruce
AU - Liu, Changhui
AU - Zheng, Weiguo
AU - Li, Fayuan
AU - Ge, Binwen
N1 - Funding Information:
The authors would like to thank the donors who generously provided samples for this validation study and thank the support from the Ministry of Public Security application innovation projects (No. 2011YYCXGDST076 ) as well as Science and Technology project of Guangdong Province (No. 2010A060801001 ). We would also like to express our thanks to Dr. George Yang for his guidance on the instruments.
PY - 2014/7
Y1 - 2014/7
N2 - The EX20+4Y System is a polymerase chain reaction (PCR)-based amplification kit that enables typing of 19 autosomal short tandem repeat (STR) loci (i.e., CSF1PO, D13S317, D16S539, D18S51, D21S11, D3S1358, D5S818, D7S820, D8S1179, FGA, TH01, TPOX, vWA, Penta D, Penta E, D2S1338, D19S433, D12S391, D6S1043), four widely used Y chromosome-specific STR (Y-STR) loci (DYS458, DYS456, DYS391, DYS635), and amelogenin. In this study, this multiplex system was validated for sensitivity of detection, DNA mixtures, inhibitor tolerance, species specificity based on the Scientific Working Group on DNA Analysis methods (SWGDAM) developmental validation guidelines, and the Chinese criteria for the human fluorescent STR multiplex PCR reagent. The results show that the EX20+4 System is a robust and reliable amplification kit which can be used for human identification testing.
AB - The EX20+4Y System is a polymerase chain reaction (PCR)-based amplification kit that enables typing of 19 autosomal short tandem repeat (STR) loci (i.e., CSF1PO, D13S317, D16S539, D18S51, D21S11, D3S1358, D5S818, D7S820, D8S1179, FGA, TH01, TPOX, vWA, Penta D, Penta E, D2S1338, D19S433, D12S391, D6S1043), four widely used Y chromosome-specific STR (Y-STR) loci (DYS458, DYS456, DYS391, DYS635), and amelogenin. In this study, this multiplex system was validated for sensitivity of detection, DNA mixtures, inhibitor tolerance, species specificity based on the Scientific Working Group on DNA Analysis methods (SWGDAM) developmental validation guidelines, and the Chinese criteria for the human fluorescent STR multiplex PCR reagent. The results show that the EX20+4 System is a robust and reliable amplification kit which can be used for human identification testing.
KW - DNA typing
KW - Developmental validation
KW - Forensic science
KW - Multiplexing
KW - Short tandem repeat (STR)
UR - http://www.scopus.com/inward/record.url?scp=84899997723&partnerID=8YFLogxK
U2 - 10.1016/j.fsigen.2014.03.001
DO - 10.1016/j.fsigen.2014.03.001
M3 - Article
C2 - 24815370
AN - SCOPUS:84899997723
SN - 1872-4973
VL - 11
SP - 207
EP - 213
JO - Forensic Science International: Genetics
JF - Forensic Science International: Genetics
IS - 1
ER -