Development of highly sensitive and specific mRNA multiplex system (XCYR1) for forensic human body fluids and tissues identification

Yan Xu, Jianhui Xie, Yu Cao, Huaigu Zhou, Yuan Ping, Liankang Chen, Lihua Gu, Wei Hu, Gang Bi, Jianye Ge, Xin Chen, Ziqin Zhao

Research output: Contribution to journalArticle

33 Scopus citations

Abstract

The identification of human body fluids or tissues through mRNA-based profiling is very useful for forensic investigations. Previous studies have shown mRNA biomarkers are effective to identify the origin of biological samples. In this study, we selected 16 tissue specific biomarkers to evaluate their specificities and sensitivities for human body fluids and tissues identification, including porphobilinogen deaminase (PBGD), hemoglobin beta (HBB) and Glycophorin A (GLY) for circulatory blood, protamine 2 (PRM2) and transglutaminase 4 (TGM4) for semen, mucin 4 (MUC4) and human beta defensin 1(HBD1) for vaginal secretion, matrix metalloproteinases 7 and 11 (MMP7 and MMP11) for menstrual blood, keratin 4(KRT4) for oral mucosa, loricrin (LOR) and cystatin 6 (CST6) for skin, histatin 3(HTN3) for saliva, statherin (STATH) for nasal secretion, dermcidin (DCD) for sweat and uromodulin (UMOD) for urine. The above mentioned ten common forensic body fluids or tissues were used in the evaluation. Based on the evaluation, a reverse transcription (RT) PCR multiplex assay, XCYR1, which includes 12 biomarkers (i.e., HBB, GLY, HTN3, PRM2, KRT4, MMP11, MUC4, DCD, UMOD, MMP7, TGM4, and STATH) and 2 housekeeping genes [i.e., glyceraldehyde-3-phosphate dehydrogenase (GAPDH) and 18SrRNA], was developed. This assay was further validated with real casework samples and mock samples (with both single source and mixture) and it was approved that XCYR1 is effective to identify common body fluids or tissues (i.e., circulatory blood, saliva, semen, vaginal secretion, menstrual blood, oral mucosa, nasal secretion, sweat and urine) in forensic casework samples.

Original languageEnglish
Article numbere100123
JournalPLoS ONE
Volume9
Issue number7
DOIs
StatePublished - 3 Jul 2014

Fingerprint Dive into the research topics of 'Development of highly sensitive and specific mRNA multiplex system (XCYR1) for forensic human body fluids and tissues identification'. Together they form a unique fingerprint.

  • Cite this

    Xu, Y., Xie, J., Cao, Y., Zhou, H., Ping, Y., Chen, L., Gu, L., Hu, W., Bi, G., Ge, J., Chen, X., & Zhao, Z. (2014). Development of highly sensitive and specific mRNA multiplex system (XCYR1) for forensic human body fluids and tissues identification. PLoS ONE, 9(7), [e100123]. https://doi.org/10.1371/journal.pone.0100123