Development of DNA probes for fingerprinting Aspergillus fumigatus

H. Girardin, J. P. Latge, Srikantha Thyagarajan, B. Morrow, D. R. Soll

Research output: Contribution to journalArticle

112 Citations (Scopus)

Abstract

Several different DNA fragments containing nonribosomal repetitive sequences have been isolated from the genome of Aspergillus fumigatus and tested as potential DNA fingerprinting probes. Eight of these clones generate 19 or more bands when hybridized to EcoRI-digested DNA of a reference strain in Southern blots, and they fall into four families. Individual clones from two families were tested and were found to generate complex Southern blot hybridization patterns which are stable within a single strain over many generations, which vary among unrelated strains, and which are amenable to computer-assisted analyses involving large numbers of strains in epidemiological studies. Clones from three of the families clustered a majority of test strains in a similar fashion in individual dendrograms based on similarity coefficients computed from band positions in Southern blot hybridization patterns. These clones therefore fulfill the major requisites for effective DNA fingerprinting probes.

Original languageEnglish
Pages (from-to)1547-1554
Number of pages8
JournalJournal of Clinical Microbiology
Volume31
Issue number6
StatePublished - 1 Jan 1993

Fingerprint

Aspergillus fumigatus
DNA Fingerprinting
DNA Probes
Clone Cells
Southern Blotting
Nucleic Acid Repetitive Sequences
DNA
Epidemiologic Studies
Genome

Cite this

Girardin, H., Latge, J. P., Thyagarajan, S., Morrow, B., & Soll, D. R. (1993). Development of DNA probes for fingerprinting Aspergillus fumigatus. Journal of Clinical Microbiology, 31(6), 1547-1554.
Girardin, H. ; Latge, J. P. ; Thyagarajan, Srikantha ; Morrow, B. ; Soll, D. R. / Development of DNA probes for fingerprinting Aspergillus fumigatus. In: Journal of Clinical Microbiology. 1993 ; Vol. 31, No. 6. pp. 1547-1554.
@article{6d6f60074d2c476f8252de70c3630e19,
title = "Development of DNA probes for fingerprinting Aspergillus fumigatus",
abstract = "Several different DNA fragments containing nonribosomal repetitive sequences have been isolated from the genome of Aspergillus fumigatus and tested as potential DNA fingerprinting probes. Eight of these clones generate 19 or more bands when hybridized to EcoRI-digested DNA of a reference strain in Southern blots, and they fall into four families. Individual clones from two families were tested and were found to generate complex Southern blot hybridization patterns which are stable within a single strain over many generations, which vary among unrelated strains, and which are amenable to computer-assisted analyses involving large numbers of strains in epidemiological studies. Clones from three of the families clustered a majority of test strains in a similar fashion in individual dendrograms based on similarity coefficients computed from band positions in Southern blot hybridization patterns. These clones therefore fulfill the major requisites for effective DNA fingerprinting probes.",
author = "H. Girardin and Latge, {J. P.} and Srikantha Thyagarajan and B. Morrow and Soll, {D. R.}",
year = "1993",
month = "1",
day = "1",
language = "English",
volume = "31",
pages = "1547--1554",
journal = "Journal of Clinical Microbiology",
issn = "0095-1137",
publisher = "American Society for Microbiology",
number = "6",

}

Girardin, H, Latge, JP, Thyagarajan, S, Morrow, B & Soll, DR 1993, 'Development of DNA probes for fingerprinting Aspergillus fumigatus', Journal of Clinical Microbiology, vol. 31, no. 6, pp. 1547-1554.

Development of DNA probes for fingerprinting Aspergillus fumigatus. / Girardin, H.; Latge, J. P.; Thyagarajan, Srikantha; Morrow, B.; Soll, D. R.

In: Journal of Clinical Microbiology, Vol. 31, No. 6, 01.01.1993, p. 1547-1554.

Research output: Contribution to journalArticle

TY - JOUR

T1 - Development of DNA probes for fingerprinting Aspergillus fumigatus

AU - Girardin, H.

AU - Latge, J. P.

AU - Thyagarajan, Srikantha

AU - Morrow, B.

AU - Soll, D. R.

PY - 1993/1/1

Y1 - 1993/1/1

N2 - Several different DNA fragments containing nonribosomal repetitive sequences have been isolated from the genome of Aspergillus fumigatus and tested as potential DNA fingerprinting probes. Eight of these clones generate 19 or more bands when hybridized to EcoRI-digested DNA of a reference strain in Southern blots, and they fall into four families. Individual clones from two families were tested and were found to generate complex Southern blot hybridization patterns which are stable within a single strain over many generations, which vary among unrelated strains, and which are amenable to computer-assisted analyses involving large numbers of strains in epidemiological studies. Clones from three of the families clustered a majority of test strains in a similar fashion in individual dendrograms based on similarity coefficients computed from band positions in Southern blot hybridization patterns. These clones therefore fulfill the major requisites for effective DNA fingerprinting probes.

AB - Several different DNA fragments containing nonribosomal repetitive sequences have been isolated from the genome of Aspergillus fumigatus and tested as potential DNA fingerprinting probes. Eight of these clones generate 19 or more bands when hybridized to EcoRI-digested DNA of a reference strain in Southern blots, and they fall into four families. Individual clones from two families were tested and were found to generate complex Southern blot hybridization patterns which are stable within a single strain over many generations, which vary among unrelated strains, and which are amenable to computer-assisted analyses involving large numbers of strains in epidemiological studies. Clones from three of the families clustered a majority of test strains in a similar fashion in individual dendrograms based on similarity coefficients computed from band positions in Southern blot hybridization patterns. These clones therefore fulfill the major requisites for effective DNA fingerprinting probes.

UR - http://www.scopus.com/inward/record.url?scp=0027167682&partnerID=8YFLogxK

M3 - Article

C2 - 8100242

AN - SCOPUS:0027167682

VL - 31

SP - 1547

EP - 1554

JO - Journal of Clinical Microbiology

JF - Journal of Clinical Microbiology

SN - 0095-1137

IS - 6

ER -