Development of capillary zone electrophoresis-electrospray ionization-mass spectrometry for the determination of lamotrigine in human plasma

Jack Zheng, Michael W. Jann, Yuen Yi Hon, Shahab A. Shamsi

Research output: Contribution to journalArticle

42 Citations (Scopus)

Abstract

A method of coupling capillary zone electrophoresis (CZE) with electrospray ionization-mass spectrometry (ESI-MS) detection has been developed for monitoring an antiepileptic drug, lamotrigine (LTG) in human plasma. The CZE-MS was developed in three stages: (i) CZE separation and ESI-MS detection of LTG and tyramine (TRM, internal standard) were simultaneously optimized by studying the influence of CZE background electrolyte (BGE) pH, BGE ionic strength, and nebulizer pressure of the MS sprayer; (ii) sheath liquid parameters, such as pH, ionic strength, organic modifier content, and flow rate of the sheath liquid, were systematically varied under optimum CZE-MS conditions developed in the first stage; (iii) MS sprayer chamber parameters (drying gas temperature and drying gas flow rate) were varied for the best MS detection of LTG. The developed assay was finally applied for the determination of LTG in plasma samples. The linear range of LTG in plasma sample assay was between 0.1-5.0 μg/mL with a limit of detection as low as 0.05 μg/mL and run time less than 6 min. Finally, the concentration-time profile of LTG in human plasma sample was found to correlate well when CZE-ESI-MS was compared to a more established method of high-performance liquid chromatography with ultraviolet detection.

Original languageEnglish
Pages (from-to)2033-2043
Number of pages11
JournalElectrophoresis
Volume25
Issue number13
DOIs
StatePublished - 1 Jul 2004

Fingerprint

Plasma (human)
Electrospray ionization
Electrospray Ionization Mass Spectrometry
Capillary Electrophoresis
Electrophoresis
Mass spectrometry
Ionic strength
Osmolar Concentration
Electrolytes
Assays
Drying
Gases
Flow rate
Plasmas
Tyramine
Nebulizers and Vaporizers
High performance liquid chromatography
Liquids
Anticonvulsants
Flow of gases

Keywords

  • Capillary zone electrophoresis
  • Electrospray ionization-mass spectrometry
  • Lamotrigine
  • Therapeutic drug monitoring

Cite this

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title = "Development of capillary zone electrophoresis-electrospray ionization-mass spectrometry for the determination of lamotrigine in human plasma",
abstract = "A method of coupling capillary zone electrophoresis (CZE) with electrospray ionization-mass spectrometry (ESI-MS) detection has been developed for monitoring an antiepileptic drug, lamotrigine (LTG) in human plasma. The CZE-MS was developed in three stages: (i) CZE separation and ESI-MS detection of LTG and tyramine (TRM, internal standard) were simultaneously optimized by studying the influence of CZE background electrolyte (BGE) pH, BGE ionic strength, and nebulizer pressure of the MS sprayer; (ii) sheath liquid parameters, such as pH, ionic strength, organic modifier content, and flow rate of the sheath liquid, were systematically varied under optimum CZE-MS conditions developed in the first stage; (iii) MS sprayer chamber parameters (drying gas temperature and drying gas flow rate) were varied for the best MS detection of LTG. The developed assay was finally applied for the determination of LTG in plasma samples. The linear range of LTG in plasma sample assay was between 0.1-5.0 μg/mL with a limit of detection as low as 0.05 μg/mL and run time less than 6 min. Finally, the concentration-time profile of LTG in human plasma sample was found to correlate well when CZE-ESI-MS was compared to a more established method of high-performance liquid chromatography with ultraviolet detection.",
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Development of capillary zone electrophoresis-electrospray ionization-mass spectrometry for the determination of lamotrigine in human plasma. / Zheng, Jack; Jann, Michael W.; Hon, Yuen Yi; Shamsi, Shahab A.

In: Electrophoresis, Vol. 25, No. 13, 01.07.2004, p. 2033-2043.

Research output: Contribution to journalArticle

TY - JOUR

T1 - Development of capillary zone electrophoresis-electrospray ionization-mass spectrometry for the determination of lamotrigine in human plasma

AU - Zheng, Jack

AU - Jann, Michael W.

AU - Hon, Yuen Yi

AU - Shamsi, Shahab A.

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