Development of biodegradable nanocarriers loaded with a monoclonal antibody

Andrew Gdowski, Amalendu Ranjan, Anindita Mukerjee, Jamboor Vishwanatha

Research output: Contribution to journalArticle

14 Citations (Scopus)

Abstract

Treatments utilizing monoclonal antibody therapeutics against intracellular protein-protein interactions in cancer cells have been hampered by several factors, including poor intracellular uptake and rapid lysosomal degradation. Our current work examines the feasibility of encapsulating monoclonal antibodies within poly(lactic-co-glycolic acid) (PLGA) nanoparticles using a water/oil/water double emulsion solvent evaporation technique. This method can be used to prepare protective polymeric nanoparticles for transporting functional antibodies to the cytoplasmic compartment of cancer cells. Nanoparticles were formulated and then characterized using a number of physical and biological parameters. The average nanoparticle size ranged from 221 to 252 nm with a low polydispersity index. Encapsulation efficiency of 16%–22% and antibody loading of 0.3%–1.12% were observed. The antibody molecules were released from the nanoparticles in a sustained manner and upon release maintained functionality. Our studies achieved successful formulation of antibody loaded polymeric nanoparticles, thus indicating that a PLGA-based antibody nanoformulation is a promising intracellular delivery vehicle for a large number of new intracellular antibody targets in cancer cells.

Original languageEnglish
Pages (from-to)3990-3995
Number of pages6
JournalInternational journal of molecular sciences
Volume16
Issue number2
DOIs
StatePublished - 12 Feb 2015

Fingerprint

Monoclonal antibodies
antibodies
Antibodies
Nanoparticles
Monoclonal Antibodies
nanoparticles
Cells
cancer
Proteins
Neoplasms
Acids
Water
Polydispersity
Emulsions
proteins
Encapsulation
acids
encapsulating
Oils
Evaporation

Keywords

  • Antibody
  • Cancer
  • Formulation
  • Nanoparticle
  • PLGA
  • Therapy

Cite this

@article{dfd1cfc314e14dbb8b2b94ffb499b285,
title = "Development of biodegradable nanocarriers loaded with a monoclonal antibody",
abstract = "Treatments utilizing monoclonal antibody therapeutics against intracellular protein-protein interactions in cancer cells have been hampered by several factors, including poor intracellular uptake and rapid lysosomal degradation. Our current work examines the feasibility of encapsulating monoclonal antibodies within poly(lactic-co-glycolic acid) (PLGA) nanoparticles using a water/oil/water double emulsion solvent evaporation technique. This method can be used to prepare protective polymeric nanoparticles for transporting functional antibodies to the cytoplasmic compartment of cancer cells. Nanoparticles were formulated and then characterized using a number of physical and biological parameters. The average nanoparticle size ranged from 221 to 252 nm with a low polydispersity index. Encapsulation efficiency of 16{\%}–22{\%} and antibody loading of 0.3{\%}–1.12{\%} were observed. The antibody molecules were released from the nanoparticles in a sustained manner and upon release maintained functionality. Our studies achieved successful formulation of antibody loaded polymeric nanoparticles, thus indicating that a PLGA-based antibody nanoformulation is a promising intracellular delivery vehicle for a large number of new intracellular antibody targets in cancer cells.",
keywords = "Antibody, Cancer, Formulation, Nanoparticle, PLGA, Therapy",
author = "Andrew Gdowski and Amalendu Ranjan and Anindita Mukerjee and Jamboor Vishwanatha",
year = "2015",
month = "2",
day = "12",
doi = "10.3390/ijms16023990",
language = "English",
volume = "16",
pages = "3990--3995",
journal = "International Journal of Molecular Sciences",
issn = "1661-6596",
publisher = "Multidisciplinary Digital Publishing Institute (MDPI)",
number = "2",

}

Development of biodegradable nanocarriers loaded with a monoclonal antibody. / Gdowski, Andrew; Ranjan, Amalendu; Mukerjee, Anindita; Vishwanatha, Jamboor.

In: International journal of molecular sciences, Vol. 16, No. 2, 12.02.2015, p. 3990-3995.

Research output: Contribution to journalArticle

TY - JOUR

T1 - Development of biodegradable nanocarriers loaded with a monoclonal antibody

AU - Gdowski, Andrew

AU - Ranjan, Amalendu

AU - Mukerjee, Anindita

AU - Vishwanatha, Jamboor

PY - 2015/2/12

Y1 - 2015/2/12

N2 - Treatments utilizing monoclonal antibody therapeutics against intracellular protein-protein interactions in cancer cells have been hampered by several factors, including poor intracellular uptake and rapid lysosomal degradation. Our current work examines the feasibility of encapsulating monoclonal antibodies within poly(lactic-co-glycolic acid) (PLGA) nanoparticles using a water/oil/water double emulsion solvent evaporation technique. This method can be used to prepare protective polymeric nanoparticles for transporting functional antibodies to the cytoplasmic compartment of cancer cells. Nanoparticles were formulated and then characterized using a number of physical and biological parameters. The average nanoparticle size ranged from 221 to 252 nm with a low polydispersity index. Encapsulation efficiency of 16%–22% and antibody loading of 0.3%–1.12% were observed. The antibody molecules were released from the nanoparticles in a sustained manner and upon release maintained functionality. Our studies achieved successful formulation of antibody loaded polymeric nanoparticles, thus indicating that a PLGA-based antibody nanoformulation is a promising intracellular delivery vehicle for a large number of new intracellular antibody targets in cancer cells.

AB - Treatments utilizing monoclonal antibody therapeutics against intracellular protein-protein interactions in cancer cells have been hampered by several factors, including poor intracellular uptake and rapid lysosomal degradation. Our current work examines the feasibility of encapsulating monoclonal antibodies within poly(lactic-co-glycolic acid) (PLGA) nanoparticles using a water/oil/water double emulsion solvent evaporation technique. This method can be used to prepare protective polymeric nanoparticles for transporting functional antibodies to the cytoplasmic compartment of cancer cells. Nanoparticles were formulated and then characterized using a number of physical and biological parameters. The average nanoparticle size ranged from 221 to 252 nm with a low polydispersity index. Encapsulation efficiency of 16%–22% and antibody loading of 0.3%–1.12% were observed. The antibody molecules were released from the nanoparticles in a sustained manner and upon release maintained functionality. Our studies achieved successful formulation of antibody loaded polymeric nanoparticles, thus indicating that a PLGA-based antibody nanoformulation is a promising intracellular delivery vehicle for a large number of new intracellular antibody targets in cancer cells.

KW - Antibody

KW - Cancer

KW - Formulation

KW - Nanoparticle

KW - PLGA

KW - Therapy

UR - http://www.scopus.com/inward/record.url?scp=84922927735&partnerID=8YFLogxK

U2 - 10.3390/ijms16023990

DO - 10.3390/ijms16023990

M3 - Article

C2 - 25690029

AN - SCOPUS:84922927735

VL - 16

SP - 3990

EP - 3995

JO - International Journal of Molecular Sciences

JF - International Journal of Molecular Sciences

SN - 1661-6596

IS - 2

ER -