Development and validation of a multiplexed Y-chromosome STR genotyping system, Y-PLEX™6, for forensic casework

Sudhir K. Sinha, Bruce Budowle, Santosh S. Arcot, Stephen L. Richey, Ranajit Chakraborty, Mary D. Jones, Patrick W. Wojtkiewicz, Dolores A. Schoenbauer, Ann Marie Gross, Siddhartha K. Sinha, Jaiprakash G. Shewale

Research output: Contribution to journalArticle

39 Scopus citations

Abstract

A Y-chromosome multiplex polymerase chain reaction (PCR) amplification kit, known as Y-PLEX™6, has been developed for use in human identification. The Y-PLEX™6 kit enables simultaneous amplification of six polymorphic short tandem repeat (STR) loci located on the non-recombinant region of the human Y-chromosome. These loci are: DYS393, DYS19, DYS389II, DYS390, DYS391, and DYS385. Our studies show that as little as 0.2 ng of template DNA can be used for analysis. The specificity of the amplification reaction enabled analysis of male DNA in a male:female DNA mixture at a ratio of 1:125. Among the six Y-STR loci, the maximum mean stutter percentage was 11.9 for allele at DYS389II locus. Attempts at amplification of DNA from various animal sources revealed that the Y-PLEX™6 primers are human specific. Details of the development of the kit, generation and description of the allelic ladders, and validation of the multiplex PCR are presented. In addition, Y-STR allele and haplotype frequencies in three populations have been investigated. The data indicate that results obtained using the Y-PLEX™6 kit are robust, sensitive, and reliable and can be used in human forensic and male lineage identification cases.

Original languageEnglish
Pages (from-to)93-103
Number of pages11
JournalJournal of Forensic Sciences
Volume48
Issue number1
DOIs
StatePublished - Jan 2003

Keywords

  • DNA typing
  • DYS19
  • DYS385
  • DYS389II
  • DYS390
  • DYS391
  • DYS393
  • Forensic science
  • Human identification
  • Multiplex
  • Polymerase chain reaction
  • Short tandem repeats
  • Y-PLEX
  • Y-STR
  • Y-chromosome

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    Sinha, S. K., Budowle, B., Arcot, S. S., Richey, S. L., Chakraborty, R., Jones, M. D., Wojtkiewicz, P. W., Schoenbauer, D. A., Gross, A. M., Sinha, S. K., & Shewale, J. G. (2003). Development and validation of a multiplexed Y-chromosome STR genotyping system, Y-PLEX™6, for forensic casework. Journal of Forensic Sciences, 48(1), 93-103. https://doi.org/10.1520/jfs2002068